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Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells

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Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells. / Weinberger, Florian; Breckwoldt, Kaja; Pecha, Simon; Kelly, Allen; Geertz, Birgit; Starbatty, Jutta; Yorgan, Timur; Cheng, Kai-Hung; Lessmann, Katrin; Stolen, Tomas; Scherrer-Crosbie, Marielle; Smith, Godfrey; Reichenspurner, Hermann; Hansen, Arne; Eschenhagen, Thomas.

In: SCI TRANSL MED, Vol. 8, No. 363, 02.11.2016, p. 363ra148.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Weinberger, F, Breckwoldt, K, Pecha, S, Kelly, A, Geertz, B, Starbatty, J, Yorgan, T, Cheng, K-H, Lessmann, K, Stolen, T, Scherrer-Crosbie, M, Smith, G, Reichenspurner, H, Hansen, A & Eschenhagen, T 2016, 'Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells', SCI TRANSL MED, vol. 8, no. 363, pp. 363ra148. https://doi.org/10.1126/scitranslmed.aaf8781

APA

Weinberger, F., Breckwoldt, K., Pecha, S., Kelly, A., Geertz, B., Starbatty, J., Yorgan, T., Cheng, K-H., Lessmann, K., Stolen, T., Scherrer-Crosbie, M., Smith, G., Reichenspurner, H., Hansen, A., & Eschenhagen, T. (2016). Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells. SCI TRANSL MED, 8(363), 363ra148. https://doi.org/10.1126/scitranslmed.aaf8781

Vancouver

Weinberger F, Breckwoldt K, Pecha S, Kelly A, Geertz B, Starbatty J et al. Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells. SCI TRANSL MED. 2016 Nov 2;8(363):363ra148. https://doi.org/10.1126/scitranslmed.aaf8781

Bibtex

@article{bd56a0d7703e4666945e4abf2542c9db,
title = "Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells",
abstract = "Myocardial injury results in a loss of contractile tissue mass that, in the absence of efficient regeneration, is essentially irreversible. Transplantation of human pluripotent stem cell-derived cardiomyocytes has beneficial but variable effects. We created human engineered heart tissue (hEHT) strips from human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hiPSC-derived endothelial cells. The hEHTs were transplanted onto large defects (22% of the left ventricular wall, 35% decline in left ventricular function) of guinea pig hearts 7 days after cryoinjury, and the results were compared with those obtained with human endothelial cell patches (hEETs) or cell-free patches. Twenty-eight days after transplantation, the hearts repaired with hEHT strips exhibited, within the scar, human heart muscle grafts, which had remuscularized 12% of the infarct area. These grafts showed cardiomyocyte proliferation, vascularization, and evidence for electrical coupling to the intact heart tissue in a subset of engrafted hearts. hEHT strips improved left ventricular function by 31% compared to that before implantation, whereas the hEET or cell-free patches had no effect. Together, our study demonstrates that three-dimensional human heart muscle constructs can repair the injured heart.POM-Newsletter",
author = "Florian Weinberger and Kaja Breckwoldt and Simon Pecha and Allen Kelly and Birgit Geertz and Jutta Starbatty and Timur Yorgan and Kai-Hung Cheng and Katrin Lessmann and Tomas Stolen and Marielle Scherrer-Crosbie and Godfrey Smith and Hermann Reichenspurner and Arne Hansen and Thomas Eschenhagen",
note = "Copyright {\textcopyright} 2016, American Association for the Advancement of Science.",
year = "2016",
month = nov,
day = "2",
doi = "10.1126/scitranslmed.aaf8781",
language = "English",
volume = "8",
pages = "363ra148",
journal = "SCI TRANSL MED",
issn = "1946-6234",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "363",

}

RIS

TY - JOUR

T1 - Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells

AU - Weinberger, Florian

AU - Breckwoldt, Kaja

AU - Pecha, Simon

AU - Kelly, Allen

AU - Geertz, Birgit

AU - Starbatty, Jutta

AU - Yorgan, Timur

AU - Cheng, Kai-Hung

AU - Lessmann, Katrin

AU - Stolen, Tomas

AU - Scherrer-Crosbie, Marielle

AU - Smith, Godfrey

AU - Reichenspurner, Hermann

AU - Hansen, Arne

AU - Eschenhagen, Thomas

N1 - Copyright © 2016, American Association for the Advancement of Science.

PY - 2016/11/2

Y1 - 2016/11/2

N2 - Myocardial injury results in a loss of contractile tissue mass that, in the absence of efficient regeneration, is essentially irreversible. Transplantation of human pluripotent stem cell-derived cardiomyocytes has beneficial but variable effects. We created human engineered heart tissue (hEHT) strips from human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hiPSC-derived endothelial cells. The hEHTs were transplanted onto large defects (22% of the left ventricular wall, 35% decline in left ventricular function) of guinea pig hearts 7 days after cryoinjury, and the results were compared with those obtained with human endothelial cell patches (hEETs) or cell-free patches. Twenty-eight days after transplantation, the hearts repaired with hEHT strips exhibited, within the scar, human heart muscle grafts, which had remuscularized 12% of the infarct area. These grafts showed cardiomyocyte proliferation, vascularization, and evidence for electrical coupling to the intact heart tissue in a subset of engrafted hearts. hEHT strips improved left ventricular function by 31% compared to that before implantation, whereas the hEET or cell-free patches had no effect. Together, our study demonstrates that three-dimensional human heart muscle constructs can repair the injured heart.POM-Newsletter

AB - Myocardial injury results in a loss of contractile tissue mass that, in the absence of efficient regeneration, is essentially irreversible. Transplantation of human pluripotent stem cell-derived cardiomyocytes has beneficial but variable effects. We created human engineered heart tissue (hEHT) strips from human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hiPSC-derived endothelial cells. The hEHTs were transplanted onto large defects (22% of the left ventricular wall, 35% decline in left ventricular function) of guinea pig hearts 7 days after cryoinjury, and the results were compared with those obtained with human endothelial cell patches (hEETs) or cell-free patches. Twenty-eight days after transplantation, the hearts repaired with hEHT strips exhibited, within the scar, human heart muscle grafts, which had remuscularized 12% of the infarct area. These grafts showed cardiomyocyte proliferation, vascularization, and evidence for electrical coupling to the intact heart tissue in a subset of engrafted hearts. hEHT strips improved left ventricular function by 31% compared to that before implantation, whereas the hEET or cell-free patches had no effect. Together, our study demonstrates that three-dimensional human heart muscle constructs can repair the injured heart.POM-Newsletter

U2 - 10.1126/scitranslmed.aaf8781

DO - 10.1126/scitranslmed.aaf8781

M3 - SCORING: Journal article

C2 - 27807283

VL - 8

SP - 363ra148

JO - SCI TRANSL MED

JF - SCI TRANSL MED

SN - 1946-6234

IS - 363

ER -