Carbonic anhydrase II is an AP-1 target gene in osteoclasts

J P David; M Rincon; L Neff; W C Horne; R Baron

doi:10.1002/jcp.1099

Carbonic anhydrase II is an AP-1 target gene in osteoclasts

Standard

Carbonic anhydrase II is an AP-1 target gene in osteoclasts. / David, J P; Rincon, M; Neff, L; Horne, W C; Baron, R.

In: J CELL PHYSIOL, Vol. 188, No. 1, 01.07.2001, p. 89-97.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

David, JP, Rincon, M, Neff, L, Horne, WC & Baron, R 2001, 'Carbonic anhydrase II is an AP-1 target gene in osteoclasts', J CELL PHYSIOL, vol. 188, no. 1, pp. 89-97. https://doi.org/10.1002/jcp.1099

APA

David, J. P., Rincon, M., Neff, L., Horne, W. C., & Baron, R. (2001). Carbonic anhydrase II is an AP-1 target gene in osteoclasts. J CELL PHYSIOL, 188(1), 89-97. https://doi.org/10.1002/jcp.1099

Vancouver

David JP, Rincon M, Neff L, Horne WC, Baron R. Carbonic anhydrase II is an AP-1 target gene in osteoclasts. J CELL PHYSIOL. 2001 Jul 1;188(1):89-97. https://doi.org/10.1002/jcp.1099

Bibtex

@article{99f2b948ddac4e679043509eb2327bf1,

title = "Carbonic anhydrase II is an AP-1 target gene in osteoclasts",

abstract = "c-Fos, a member of the AP-1 family of transcription factors, is necessary for osteoclast differentiation but to date, none of the osteoclast-phenotypic markers have been identified as AP-1 target genes. Here, we demonstrate that carbonic anhydrase II (CA II), an enzyme necessary for osteoclast activity, is transcriptionally upregulated by c-Fos/AP-1. A functional AP-1 binding site is present in the CA II promoter and is necessary for this regulation. Furthermore, we show that AP-1 binding activity, mainly composed of Fra-2 and JunD, is induced by treatment of bone marrow cultures with the osteoclastogenic hormone 1,25 dihydroxyvitamin D(3). Fra-2 and JunD are found in mature osteoclasts as well. Thus, our data demonstrate that cFos/AP-1 can directly regulate the expression of this osteoclast marker and that AP-1 activity is upregulated in osteoclast progenitors in response to osteoclastogenic signals.",

keywords = "Animals, Binding Sites, Bone Marrow Cells, Calcitriol, Carbonic Anhydrases, Cells, Cultured, Chickens, DNA-Binding Proteins, Fos-Related Antigen-2, Humans, Immunohistochemistry, Nuclear Proteins, Osteoclasts, Promoter Regions, Genetic, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, Rats, Tetradecanoylphorbol Acetate, Transcription Factor AP-1, Transcription Factors, Transfection",

author = "David, {J P} and M Rincon and L Neff and Horne, {W C} and R Baron",

year = "2001",

month = jul,

day = "1",

doi = "10.1002/jcp.1099",

language = "English",

volume = "188",

pages = "89--97",

journal = "J CELL PHYSIOL",

issn = "0021-9541",

publisher = "Wiley-Liss Inc.",

number = "1",

}

RIS

TY - JOUR

T1 - Carbonic anhydrase II is an AP-1 target gene in osteoclasts

AU - David, J P

AU - Rincon, M

AU - Neff, L

AU - Horne, W C

AU - Baron, R

PY - 2001/7/1

Y1 - 2001/7/1

N2 - c-Fos, a member of the AP-1 family of transcription factors, is necessary for osteoclast differentiation but to date, none of the osteoclast-phenotypic markers have been identified as AP-1 target genes. Here, we demonstrate that carbonic anhydrase II (CA II), an enzyme necessary for osteoclast activity, is transcriptionally upregulated by c-Fos/AP-1. A functional AP-1 binding site is present in the CA II promoter and is necessary for this regulation. Furthermore, we show that AP-1 binding activity, mainly composed of Fra-2 and JunD, is induced by treatment of bone marrow cultures with the osteoclastogenic hormone 1,25 dihydroxyvitamin D(3). Fra-2 and JunD are found in mature osteoclasts as well. Thus, our data demonstrate that cFos/AP-1 can directly regulate the expression of this osteoclast marker and that AP-1 activity is upregulated in osteoclast progenitors in response to osteoclastogenic signals.

AB - c-Fos, a member of the AP-1 family of transcription factors, is necessary for osteoclast differentiation but to date, none of the osteoclast-phenotypic markers have been identified as AP-1 target genes. Here, we demonstrate that carbonic anhydrase II (CA II), an enzyme necessary for osteoclast activity, is transcriptionally upregulated by c-Fos/AP-1. A functional AP-1 binding site is present in the CA II promoter and is necessary for this regulation. Furthermore, we show that AP-1 binding activity, mainly composed of Fra-2 and JunD, is induced by treatment of bone marrow cultures with the osteoclastogenic hormone 1,25 dihydroxyvitamin D(3). Fra-2 and JunD are found in mature osteoclasts as well. Thus, our data demonstrate that cFos/AP-1 can directly regulate the expression of this osteoclast marker and that AP-1 activity is upregulated in osteoclast progenitors in response to osteoclastogenic signals.

KW - Animals

KW - Binding Sites

KW - Bone Marrow Cells

KW - Calcitriol

KW - Carbonic Anhydrases

KW - Cells, Cultured

KW - Chickens

KW - DNA-Binding Proteins

KW - Fos-Related Antigen-2

KW - Humans

KW - Immunohistochemistry

KW - Nuclear Proteins

KW - Osteoclasts

KW - Promoter Regions, Genetic

KW - Proto-Oncogene Proteins c-fos

KW - Proto-Oncogene Proteins c-jun

KW - Rats

KW - Tetradecanoylphorbol Acetate

KW - Transcription Factor AP-1

KW - Transcription Factors

KW - Transfection

U2 - 10.1002/jcp.1099

DO - 10.1002/jcp.1099

M3 - SCORING: Journal article

C2 - 11382925

VL - 188

SP - 89

EP - 97

JO - J CELL PHYSIOL

JF - J CELL PHYSIOL

SN - 0021-9541

IS - 1

ER -