Carbohydrate moieties can induce mediator release: a detailed characterization of two major timothy grass pollen allergens.
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Carbohydrate moieties can induce mediator release: a detailed characterization of two major timothy grass pollen allergens. / Wicklein, Daniel; Lindner, Buko; Moll, Hermann; Kolarich, Daniel; Altmann, Friedrich; Becker, Wolf-Meinhard; Petersen, Arnd.
In: BIOL CHEM, Vol. 385, No. 5, 5, 2004, p. 397-407.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Carbohydrate moieties can induce mediator release: a detailed characterization of two major timothy grass pollen allergens.
AU - Wicklein, Daniel
AU - Lindner, Buko
AU - Moll, Hermann
AU - Kolarich, Daniel
AU - Altmann, Friedrich
AU - Becker, Wolf-Meinhard
AU - Petersen, Arnd
PY - 2004
Y1 - 2004
N2 - Specific IgE binding to carbohydrate moieties of glycosylated allergens has been known for years, but the importance of these structures for the elicitation of allergic reactions is still a matter of debate. Because of their conserved carbohydrate structures, especially N-glycans have always been prime candidates for IgE cross-reactivity between allergens from unrelated species. The aim of our study was to determine whether carbohydrate structures on glycoproteins can by themselves elucidate allergic reactions. We characterized in detail the carbohydrate moieties of the major allergens Phl p 1 and Phl p 13 of timothy grass pollen (Phleum pratense L.) by performing tryptic digests followed by HPLC, N-terminal sequencing, sugar analysis, MALDI-TOF- and ESI-ICRFT-MS. Phl p 1 contains one N-glycan with one of the two glycoforms MMXF3 and M0XF3 and a single furanosidic arabinose, which is bound to a hydroxyproline residue in direct vicinity to the N-glycan. This O-glycosylation is probably due to an arabinosylation consensus sequence found in the N-terminal part of Phl p 1 and other group 1 allergens, but displayed no IgE-reactivity. Thus, Phl p 1 is monovalent with respect to its IgE-binding carbohydrate epitopes and showed no mediator release. In contrast, the carbohydrate moiety of Phl p 13, which carries four of the same N-glycans (like Phl p 1), can cross-link IgE-receptors via carbohydrate chains and elicits IL-4 release from basophils.
AB - Specific IgE binding to carbohydrate moieties of glycosylated allergens has been known for years, but the importance of these structures for the elicitation of allergic reactions is still a matter of debate. Because of their conserved carbohydrate structures, especially N-glycans have always been prime candidates for IgE cross-reactivity between allergens from unrelated species. The aim of our study was to determine whether carbohydrate structures on glycoproteins can by themselves elucidate allergic reactions. We characterized in detail the carbohydrate moieties of the major allergens Phl p 1 and Phl p 13 of timothy grass pollen (Phleum pratense L.) by performing tryptic digests followed by HPLC, N-terminal sequencing, sugar analysis, MALDI-TOF- and ESI-ICRFT-MS. Phl p 1 contains one N-glycan with one of the two glycoforms MMXF3 and M0XF3 and a single furanosidic arabinose, which is bound to a hydroxyproline residue in direct vicinity to the N-glycan. This O-glycosylation is probably due to an arabinosylation consensus sequence found in the N-terminal part of Phl p 1 and other group 1 allergens, but displayed no IgE-reactivity. Thus, Phl p 1 is monovalent with respect to its IgE-binding carbohydrate epitopes and showed no mediator release. In contrast, the carbohydrate moiety of Phl p 13, which carries four of the same N-glycans (like Phl p 1), can cross-link IgE-receptors via carbohydrate chains and elicits IL-4 release from basophils.
KW - Humans
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Fucose/chemistry/metabolism
KW - Immunoglobulin E/immunology
KW - Allergens/chemistry/immunology
KW - Basophils/drug effects/immunology
KW - Bromelains/pharmacology
KW - Hypersensitivity/immunology
KW - Interleukin-4/metabolism
KW - Plant Proteins/chemistry/immunology
KW - Polysaccharides/chemistry/immunology/metabolism
KW - Humans
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Fucose/chemistry/metabolism
KW - Immunoglobulin E/immunology
KW - Allergens/chemistry/immunology
KW - Basophils/drug effects/immunology
KW - Bromelains/pharmacology
KW - Hypersensitivity/immunology
KW - Interleukin-4/metabolism
KW - Plant Proteins/chemistry/immunology
KW - Polysaccharides/chemistry/immunology/metabolism
M3 - SCORING: Journal article
VL - 385
SP - 397
EP - 407
JO - BIOL CHEM
JF - BIOL CHEM
SN - 1431-6730
IS - 5
M1 - 5
ER -
