Cancer cell motility is affected through 3D cell culturing and SCF/c-Kit pathway but not by X-irradiation
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Cancer cell motility is affected through 3D cell culturing and SCF/c-Kit pathway but not by X-irradiation. / Eberle, Fabian; Saulich, Miriam F; Leinberger, Florian H; Seeger, Werner; Engenhart-Cabillic, Rita; Dikomey, Ekkehard; Hänze, Jörg; Hattar, Katja; Subtil, Florentine S B.
In: RADIOTHER ONCOL, Vol. 119, No. 3, 06.2016, p. 537-43.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cancer cell motility is affected through 3D cell culturing and SCF/c-Kit pathway but not by X-irradiation
AU - Eberle, Fabian
AU - Saulich, Miriam F
AU - Leinberger, Florian H
AU - Seeger, Werner
AU - Engenhart-Cabillic, Rita
AU - Dikomey, Ekkehard
AU - Hänze, Jörg
AU - Hattar, Katja
AU - Subtil, Florentine S B
N1 - Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - BACKGROUND AND PURPOSE: Success of radiotherapy is often limited by therapy resistance and metastasis resulting from cancer cell motility. It was tested in vitro whether this cancer cell motility is affected by growth condition, active SCF/c-Kit pathway or X-irradiation.MATERIALS AND METHODS: Cell motility was measured with BioCoat™ Matrigel™ invasion chamber using four different cancer cell lines (NSCLC: H23, H520, H226 and PrCa: DU145). Cells were grown in 2D or 3D, SCF was knocked down by siRNA and cells were irradiated with 2 or 6Gy.RESULTS: All cell lines except H520 showed a 2-3-fold increase in cell motility when grown in 3D. This effect was considered to result from the EMT-like change seen when cells were grown in 3D as indicated by the enhanced expression of vimentin and N-cadherin and reduction of E-cadherin. Just the opposite trends were found for H520 cells. Knockdown of SCF was found to result in reduced cell motility for both 2D and 3D. In contrast, X-irradiation did not modulate cell motility neither under 2D nor 3D. In line with this, X-irradiation did neither induce the expression of EMT-associated genes nor SCF.CONCLUSION: X-irradiation affects neither the expression of important EMT genes such as vimentin, E-cadherin and N-cadherin nor SCF/c-Kit signaling and, as a consequence, does not alter cell motility.
AB - BACKGROUND AND PURPOSE: Success of radiotherapy is often limited by therapy resistance and metastasis resulting from cancer cell motility. It was tested in vitro whether this cancer cell motility is affected by growth condition, active SCF/c-Kit pathway or X-irradiation.MATERIALS AND METHODS: Cell motility was measured with BioCoat™ Matrigel™ invasion chamber using four different cancer cell lines (NSCLC: H23, H520, H226 and PrCa: DU145). Cells were grown in 2D or 3D, SCF was knocked down by siRNA and cells were irradiated with 2 or 6Gy.RESULTS: All cell lines except H520 showed a 2-3-fold increase in cell motility when grown in 3D. This effect was considered to result from the EMT-like change seen when cells were grown in 3D as indicated by the enhanced expression of vimentin and N-cadherin and reduction of E-cadherin. Just the opposite trends were found for H520 cells. Knockdown of SCF was found to result in reduced cell motility for both 2D and 3D. In contrast, X-irradiation did not modulate cell motility neither under 2D nor 3D. In line with this, X-irradiation did neither induce the expression of EMT-associated genes nor SCF.CONCLUSION: X-irradiation affects neither the expression of important EMT genes such as vimentin, E-cadherin and N-cadherin nor SCF/c-Kit signaling and, as a consequence, does not alter cell motility.
U2 - 10.1016/j.radonc.2016.04.036
DO - 10.1016/j.radonc.2016.04.036
M3 - SCORING: Journal article
C2 - 27178146
VL - 119
SP - 537
EP - 543
JO - RADIOTHER ONCOL
JF - RADIOTHER ONCOL
SN - 0167-8140
IS - 3
ER -
