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Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice

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Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice. / Baumeier, Christian; Kaiser, Daniel; Heeren, Jörg; Scheja, Ludger; John, Clara; Weise, Christoph; Eravci, Murat; Lagerpusch, Merit; Schulze, Gunnar; Joost, Hans-Georg; Schwenk, Robert Wolfgang; Schürmann, Annette.

In: Biochim Biophys Acta, Vol. 1851, No. 5, 05.2015, p. 566-76.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Baumeier, C, Kaiser, D, Heeren, J, Scheja, L, John, C, Weise, C, Eravci, M, Lagerpusch, M, Schulze, G, Joost, H-G, Schwenk, RW & Schürmann, A 2015, 'Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice', Biochim Biophys Acta, vol. 1851, no. 5, pp. 566-76. https://doi.org/10.1016/j.bbalip.2015.01.013

APA

Baumeier, C., Kaiser, D., Heeren, J., Scheja, L., John, C., Weise, C., Eravci, M., Lagerpusch, M., Schulze, G., Joost, H-G., Schwenk, R. W., & Schürmann, A. (2015). Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice. Biochim Biophys Acta, 1851(5), 566-76. https://doi.org/10.1016/j.bbalip.2015.01.013

Vancouver

Baumeier C, Kaiser D, Heeren J, Scheja L, John C, Weise C et al. Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice. Biochim Biophys Acta. 2015 May;1851(5):566-76. https://doi.org/10.1016/j.bbalip.2015.01.013

Bibtex

@article{0b63619dd54646e78732836a42ab670e,
title = "Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice",
abstract = "Caloric restriction and intermittent fasting are known to improve glucose homeostasis and insulin resistance in several species including humans. The aim of this study was to unravel potential mechanisms by which these interventions improve insulin sensitivity and protect from type 2 diabetes. Diabetes-susceptible New Zealand Obese mice were either 10% calorie restricted (CR) or fasted every other day (IF), and compared to ad libitum (AL) fed control mice. AL mice showed a diabetes prevalence of 43%, whereas mice under CR and IF were completely protected against hyperglycemia. Proteomic analysis of hepatic lipid droplets revealed significantly higher levels of PSMD9 (co-activator Bridge-1), MIF (macrophage migration inhibitor factor), TCEB2 (transcription elongation factor B (SIII), polypeptide 2), ACY1 (aminoacylase 1) and FABP5 (fatty acid binding protein 5), and a marked reduction of GSTA3 (glutathione S-transferase alpha 3) in samples of CR and IF mice. In addition, accumulation of diacylglycerols (DAGs) was significantly reduced in livers of IF mice (P=0.045) while CR mice showed a similar tendency (P=0.062). In particular, 9 DAG species were significantly reduced in response to IF, of which DAG-40:4 and DAG-40:7 also showed significant effects after CR. This was associated with a decreased PKCε activation and might explain the improved insulin sensitivity. In conclusion, our data indicate that protection against diabetes upon caloric restriction and intermittent fasting associates with a modulation of lipid droplet protein composition and reduction of intracellular DAG species.",
keywords = "Animals, Blood Glucose, Caloric Restriction, Diabetes Mellitus, Type 2, Diglycerides, Disease Models, Animal, Fasting, Fatty Acids, Food Deprivation, Insulin, Insulin Resistance, Lipid Droplets, Liver, Male, Mice, Obese, Muscle, Skeletal, Obesity, Oxidation-Reduction, Protein Kinase C-epsilon, Proteome, Time Factors",
author = "Christian Baumeier and Daniel Kaiser and J{\"o}rg Heeren and Ludger Scheja and Clara John and Christoph Weise and Murat Eravci and Merit Lagerpusch and Gunnar Schulze and Hans-Georg Joost and Schwenk, {Robert Wolfgang} and Annette Sch{\"u}rmann",
note = "Copyright {\textcopyright} 2015. Published by Elsevier B.V.",
year = "2015",
month = may,
doi = "10.1016/j.bbalip.2015.01.013",
language = "English",
volume = "1851",
pages = "566--76",
journal = "Biochim Biophys Acta",
issn = "0006-3002",
number = "5",

}

RIS

TY - JOUR

T1 - Caloric restriction and intermittent fasting alter hepatic lipid droplet proteome and diacylglycerol species and prevent diabetes in NZO mice

AU - Baumeier, Christian

AU - Kaiser, Daniel

AU - Heeren, Jörg

AU - Scheja, Ludger

AU - John, Clara

AU - Weise, Christoph

AU - Eravci, Murat

AU - Lagerpusch, Merit

AU - Schulze, Gunnar

AU - Joost, Hans-Georg

AU - Schwenk, Robert Wolfgang

AU - Schürmann, Annette

N1 - Copyright © 2015. Published by Elsevier B.V.

PY - 2015/5

Y1 - 2015/5

N2 - Caloric restriction and intermittent fasting are known to improve glucose homeostasis and insulin resistance in several species including humans. The aim of this study was to unravel potential mechanisms by which these interventions improve insulin sensitivity and protect from type 2 diabetes. Diabetes-susceptible New Zealand Obese mice were either 10% calorie restricted (CR) or fasted every other day (IF), and compared to ad libitum (AL) fed control mice. AL mice showed a diabetes prevalence of 43%, whereas mice under CR and IF were completely protected against hyperglycemia. Proteomic analysis of hepatic lipid droplets revealed significantly higher levels of PSMD9 (co-activator Bridge-1), MIF (macrophage migration inhibitor factor), TCEB2 (transcription elongation factor B (SIII), polypeptide 2), ACY1 (aminoacylase 1) and FABP5 (fatty acid binding protein 5), and a marked reduction of GSTA3 (glutathione S-transferase alpha 3) in samples of CR and IF mice. In addition, accumulation of diacylglycerols (DAGs) was significantly reduced in livers of IF mice (P=0.045) while CR mice showed a similar tendency (P=0.062). In particular, 9 DAG species were significantly reduced in response to IF, of which DAG-40:4 and DAG-40:7 also showed significant effects after CR. This was associated with a decreased PKCε activation and might explain the improved insulin sensitivity. In conclusion, our data indicate that protection against diabetes upon caloric restriction and intermittent fasting associates with a modulation of lipid droplet protein composition and reduction of intracellular DAG species.

AB - Caloric restriction and intermittent fasting are known to improve glucose homeostasis and insulin resistance in several species including humans. The aim of this study was to unravel potential mechanisms by which these interventions improve insulin sensitivity and protect from type 2 diabetes. Diabetes-susceptible New Zealand Obese mice were either 10% calorie restricted (CR) or fasted every other day (IF), and compared to ad libitum (AL) fed control mice. AL mice showed a diabetes prevalence of 43%, whereas mice under CR and IF were completely protected against hyperglycemia. Proteomic analysis of hepatic lipid droplets revealed significantly higher levels of PSMD9 (co-activator Bridge-1), MIF (macrophage migration inhibitor factor), TCEB2 (transcription elongation factor B (SIII), polypeptide 2), ACY1 (aminoacylase 1) and FABP5 (fatty acid binding protein 5), and a marked reduction of GSTA3 (glutathione S-transferase alpha 3) in samples of CR and IF mice. In addition, accumulation of diacylglycerols (DAGs) was significantly reduced in livers of IF mice (P=0.045) while CR mice showed a similar tendency (P=0.062). In particular, 9 DAG species were significantly reduced in response to IF, of which DAG-40:4 and DAG-40:7 also showed significant effects after CR. This was associated with a decreased PKCε activation and might explain the improved insulin sensitivity. In conclusion, our data indicate that protection against diabetes upon caloric restriction and intermittent fasting associates with a modulation of lipid droplet protein composition and reduction of intracellular DAG species.

KW - Animals

KW - Blood Glucose

KW - Caloric Restriction

KW - Diabetes Mellitus, Type 2

KW - Diglycerides

KW - Disease Models, Animal

KW - Fasting

KW - Fatty Acids

KW - Food Deprivation

KW - Insulin

KW - Insulin Resistance

KW - Lipid Droplets

KW - Liver

KW - Male

KW - Mice, Obese

KW - Muscle, Skeletal

KW - Obesity

KW - Oxidation-Reduction

KW - Protein Kinase C-epsilon

KW - Proteome

KW - Time Factors

U2 - 10.1016/j.bbalip.2015.01.013

DO - 10.1016/j.bbalip.2015.01.013

M3 - SCORING: Journal article

C2 - 25645620

VL - 1851

SP - 566

EP - 576

JO - Biochim Biophys Acta

JF - Biochim Biophys Acta

SN - 0006-3002

IS - 5

ER -