Ca(2+)-signalling in human T-lymphocytes. Potential roles for cyclic ADP-ribose and 2'-phospho-cyclic ADP-ribose.
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Ca(2+)-signalling in human T-lymphocytes. Potential roles for cyclic ADP-ribose and 2'-phospho-cyclic ADP-ribose. / Guse, A H; Da Silva, C P; Potter, B V; Mayr, Georg W.
In: ADV EXP MED BIOL, Vol. 419, 1997, p. 431-436.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Ca(2+)-signalling in human T-lymphocytes. Potential roles for cyclic ADP-ribose and 2'-phospho-cyclic ADP-ribose.
AU - Guse, A H
AU - Da Silva, C P
AU - Potter, B V
AU - Mayr, Georg W.
PY - 1997
Y1 - 1997
N2 - Intracellular Ca(2+)-signals belong to the major events transducing extracellular signals into living cells. The discovery of (i) a caffeine-sensitive intracellular Ca(2+)-pool in Jurkat T-lymphocytes [1] and (ii) cyclic adenosine diphosphoribose (cADPR) as an agent that mobilizes Ca2+ from a caffeine- and ryanodine sensitive Ca(2+)-store in sea urchin egg homogenates [2] prompted us to investigate the potential role of this compound in T-lymphocyte Ca(2+)-signalling. cADPR, as well as its 2'-phosphorylated derivative, 2'-phospho-cADPR (2'-cADPR), released Ca2+ in a dose-dependent, specific manner from intracellular, non-endoplasmic reticular stores of permeabilized Jurkat and HPB.ALL T cells. In addition, attempts were made to prove the presence of endogenous cADPR and 2'-P-cADPR by HPLC. Several HPLC protocols, including microbore-HPLC were tested resulting in the detection of endogenous cADPR by sequential separation on strong-anion exchange HPLC and reverse-phase ion-pair HPLC.
AB - Intracellular Ca(2+)-signals belong to the major events transducing extracellular signals into living cells. The discovery of (i) a caffeine-sensitive intracellular Ca(2+)-pool in Jurkat T-lymphocytes [1] and (ii) cyclic adenosine diphosphoribose (cADPR) as an agent that mobilizes Ca2+ from a caffeine- and ryanodine sensitive Ca(2+)-store in sea urchin egg homogenates [2] prompted us to investigate the potential role of this compound in T-lymphocyte Ca(2+)-signalling. cADPR, as well as its 2'-phosphorylated derivative, 2'-phospho-cADPR (2'-cADPR), released Ca2+ in a dose-dependent, specific manner from intracellular, non-endoplasmic reticular stores of permeabilized Jurkat and HPB.ALL T cells. In addition, attempts were made to prove the presence of endogenous cADPR and 2'-P-cADPR by HPLC. Several HPLC protocols, including microbore-HPLC were tested resulting in the detection of endogenous cADPR by sequential separation on strong-anion exchange HPLC and reverse-phase ion-pair HPLC.
M3 - SCORING: Zeitschriftenaufsatz
VL - 419
SP - 431
EP - 436
JO - ADV EXP MED BIOL
JF - ADV EXP MED BIOL
SN - 0065-2598
ER -
