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Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene.

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Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene. / Miletic, Hrvoje; Fischer, Yvonne; Litwak, Sara; Giroglou, Tsanan; Waerzeggers, Yannic; Winkeler, Alexandra; Li, Huongfeng; Himmelreich, Uwe; Lange, Claudia; Stenzel, Werner; Deckert, Martina; Neumann, Harald; Jacobs, Andreas H; von Laer, Dorothee.

In: MOL THER, Vol. 15, No. 7, 7, 2007, p. 1373-1381.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Miletic, H, Fischer, Y, Litwak, S, Giroglou, T, Waerzeggers, Y, Winkeler, A, Li, H, Himmelreich, U, Lange, C, Stenzel, W, Deckert, M, Neumann, H, Jacobs, AH & von Laer, D 2007, 'Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene.', MOL THER, vol. 15, no. 7, 7, pp. 1373-1381. <http://www.ncbi.nlm.nih.gov/pubmed/17457322?dopt=Citation>

APA

Miletic, H., Fischer, Y., Litwak, S., Giroglou, T., Waerzeggers, Y., Winkeler, A., Li, H., Himmelreich, U., Lange, C., Stenzel, W., Deckert, M., Neumann, H., Jacobs, A. H., & von Laer, D. (2007). Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene. MOL THER, 15(7), 1373-1381. [7]. http://www.ncbi.nlm.nih.gov/pubmed/17457322?dopt=Citation

Vancouver

Miletic H, Fischer Y, Litwak S, Giroglou T, Waerzeggers Y, Winkeler A et al. Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene. MOL THER. 2007;15(7):1373-1381. 7.

Bibtex

@article{2dbd291360a44fba86f675180b6b3e55,
title = "Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene.",
abstract = "Adult stem cells are promising cellular vehicles for therapy of malignant gliomas as they have the ability to migrate into these tumors and even track infiltrating tumor cells. However, their clinical use is limited by a low passaging capacity that impedes large-scale production. In the present study, a bone marrow-derived, highly proliferative subpopulation of mesenchymal stem cells (MSCs)-here termed bone marrow-derived tumor-infiltrating cells (BM-TICs)-was genetically modified for the treatment of malignant glioma. Upon injection into the tumor or the vicinity of the tumor, BM-TICs infiltrated solid parts as well as the border of rat 9L glioma. After intra-tumoral injection, BM-TICs expressing the thymidine kinase of herpes simplex virus (HSV-tk) and enhanced green fluorescent protein (BM-TIC-tk-GFP) were detected by non-invasive positron emission tomography (PET) using the tracer 9-[4-[(18)F]fluoro-3-hydroxymethyl)butyl]guanine ([(18)F]FHBG). A therapeutic effect was demonstrated in vitro and in vivo by BM-TICs expressing HSV-tk through bystander-mediated glioma cell killing. Therapeutic efficacy was monitored by PET as well as by magnetic resonance imaging (MRI) and strongly correlated with histological analysis. In conclusion, BM-TICs expressing a suicide gene were highly effective in the treatment of malignant glioma in a rat model and therefore hold great potential for the therapy of malignant brain tumors in humans.",
author = "Hrvoje Miletic and Yvonne Fischer and Sara Litwak and Tsanan Giroglou and Yannic Waerzeggers and Alexandra Winkeler and Huongfeng Li and Uwe Himmelreich and Claudia Lange and Werner Stenzel and Martina Deckert and Harald Neumann and Jacobs, {Andreas H} and {von Laer}, Dorothee",
year = "2007",
language = "Deutsch",
volume = "15",
pages = "1373--1381",
journal = "MOL THER",
issn = "1525-0016",
publisher = "NATURE PUBLISHING GROUP",
number = "7",

}

RIS

TY - JOUR

T1 - Bystander killing of malignant glioma by bone marrow-derived tumor-infiltrating progenitor cells expressing a suicide gene.

AU - Miletic, Hrvoje

AU - Fischer, Yvonne

AU - Litwak, Sara

AU - Giroglou, Tsanan

AU - Waerzeggers, Yannic

AU - Winkeler, Alexandra

AU - Li, Huongfeng

AU - Himmelreich, Uwe

AU - Lange, Claudia

AU - Stenzel, Werner

AU - Deckert, Martina

AU - Neumann, Harald

AU - Jacobs, Andreas H

AU - von Laer, Dorothee

PY - 2007

Y1 - 2007

N2 - Adult stem cells are promising cellular vehicles for therapy of malignant gliomas as they have the ability to migrate into these tumors and even track infiltrating tumor cells. However, their clinical use is limited by a low passaging capacity that impedes large-scale production. In the present study, a bone marrow-derived, highly proliferative subpopulation of mesenchymal stem cells (MSCs)-here termed bone marrow-derived tumor-infiltrating cells (BM-TICs)-was genetically modified for the treatment of malignant glioma. Upon injection into the tumor or the vicinity of the tumor, BM-TICs infiltrated solid parts as well as the border of rat 9L glioma. After intra-tumoral injection, BM-TICs expressing the thymidine kinase of herpes simplex virus (HSV-tk) and enhanced green fluorescent protein (BM-TIC-tk-GFP) were detected by non-invasive positron emission tomography (PET) using the tracer 9-[4-[(18)F]fluoro-3-hydroxymethyl)butyl]guanine ([(18)F]FHBG). A therapeutic effect was demonstrated in vitro and in vivo by BM-TICs expressing HSV-tk through bystander-mediated glioma cell killing. Therapeutic efficacy was monitored by PET as well as by magnetic resonance imaging (MRI) and strongly correlated with histological analysis. In conclusion, BM-TICs expressing a suicide gene were highly effective in the treatment of malignant glioma in a rat model and therefore hold great potential for the therapy of malignant brain tumors in humans.

AB - Adult stem cells are promising cellular vehicles for therapy of malignant gliomas as they have the ability to migrate into these tumors and even track infiltrating tumor cells. However, their clinical use is limited by a low passaging capacity that impedes large-scale production. In the present study, a bone marrow-derived, highly proliferative subpopulation of mesenchymal stem cells (MSCs)-here termed bone marrow-derived tumor-infiltrating cells (BM-TICs)-was genetically modified for the treatment of malignant glioma. Upon injection into the tumor or the vicinity of the tumor, BM-TICs infiltrated solid parts as well as the border of rat 9L glioma. After intra-tumoral injection, BM-TICs expressing the thymidine kinase of herpes simplex virus (HSV-tk) and enhanced green fluorescent protein (BM-TIC-tk-GFP) were detected by non-invasive positron emission tomography (PET) using the tracer 9-[4-[(18)F]fluoro-3-hydroxymethyl)butyl]guanine ([(18)F]FHBG). A therapeutic effect was demonstrated in vitro and in vivo by BM-TICs expressing HSV-tk through bystander-mediated glioma cell killing. Therapeutic efficacy was monitored by PET as well as by magnetic resonance imaging (MRI) and strongly correlated with histological analysis. In conclusion, BM-TICs expressing a suicide gene were highly effective in the treatment of malignant glioma in a rat model and therefore hold great potential for the therapy of malignant brain tumors in humans.

M3 - SCORING: Zeitschriftenaufsatz

VL - 15

SP - 1373

EP - 1381

JO - MOL THER

JF - MOL THER

SN - 1525-0016

IS - 7

M1 - 7

ER -