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BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire

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BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire. / Najafova, Zeynab; Tirado-Magallanes, Roberto; Subramaniam, Malayannan; Hossan, Tareq; Schmidt, Geske; Nagarajan, Sankari; Baumgart, Simon J; Mishra, Vivek Kumar; Bedi, Upasana; Hesse, Eric; Knapp, Stefan; Hawse, John R; Johnsen, Steven A.

In: NUCLEIC ACIDS RES, Vol. 45, No. 1, 09.01.2017, p. 127-141.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Najafova, Z, Tirado-Magallanes, R, Subramaniam, M, Hossan, T, Schmidt, G, Nagarajan, S, Baumgart, SJ, Mishra, VK, Bedi, U, Hesse, E, Knapp, S, Hawse, JR & Johnsen, SA 2017, 'BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire', NUCLEIC ACIDS RES, vol. 45, no. 1, pp. 127-141. https://doi.org/10.1093/nar/gkw826

APA

Najafova, Z., Tirado-Magallanes, R., Subramaniam, M., Hossan, T., Schmidt, G., Nagarajan, S., Baumgart, S. J., Mishra, V. K., Bedi, U., Hesse, E., Knapp, S., Hawse, J. R., & Johnsen, S. A. (2017). BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire. NUCLEIC ACIDS RES, 45(1), 127-141. https://doi.org/10.1093/nar/gkw826

Vancouver

Najafova Z, Tirado-Magallanes R, Subramaniam M, Hossan T, Schmidt G, Nagarajan S et al. BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire. NUCLEIC ACIDS RES. 2017 Jan 9;45(1):127-141. https://doi.org/10.1093/nar/gkw826

Bibtex

@article{9d47d4d48d054c4894824d6ca0f12cd2,
title = "BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire",
abstract = "Proper temporal epigenetic regulation of gene expression is essential for cell fate determination and tissue development. The Bromodomain-containing Protein-4 (BRD4) was previously shown to control the transcription of defined subsets of genes in various cell systems. In this study we examined the role of BRD4 in promoting lineage-specific gene expression and show that BRD4 is essential for osteoblast differentiation. Genome-wide analyses demonstrate that BRD4 is recruited to the transcriptional start site of differentiation-induced genes. Unexpectedly, while promoter-proximal BRD4 occupancy correlated with gene expression, genes which displayed moderate expression and promoter-proximal BRD4 occupancy were most highly regulated and sensitive to BRD4 inhibition. Therefore, we examined distal BRD4 occupancy and uncovered a specific co-localization of BRD4 with the transcription factors C/EBPb, TEAD1, FOSL2 and JUND at putative osteoblast-specific enhancers. These findings reveal the intricacies of lineage specification and provide new insight into the context-dependent functions of BRD4.",
author = "Zeynab Najafova and Roberto Tirado-Magallanes and Malayannan Subramaniam and Tareq Hossan and Geske Schmidt and Sankari Nagarajan and Baumgart, {Simon J} and Mishra, {Vivek Kumar} and Upasana Bedi and Eric Hesse and Stefan Knapp and Hawse, {John R} and Johnsen, {Steven A}",
note = "{\textcopyright} The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2017",
month = jan,
day = "9",
doi = "10.1093/nar/gkw826",
language = "English",
volume = "45",
pages = "127--141",
journal = "NUCLEIC ACIDS RES",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire

AU - Najafova, Zeynab

AU - Tirado-Magallanes, Roberto

AU - Subramaniam, Malayannan

AU - Hossan, Tareq

AU - Schmidt, Geske

AU - Nagarajan, Sankari

AU - Baumgart, Simon J

AU - Mishra, Vivek Kumar

AU - Bedi, Upasana

AU - Hesse, Eric

AU - Knapp, Stefan

AU - Hawse, John R

AU - Johnsen, Steven A

N1 - © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2017/1/9

Y1 - 2017/1/9

N2 - Proper temporal epigenetic regulation of gene expression is essential for cell fate determination and tissue development. The Bromodomain-containing Protein-4 (BRD4) was previously shown to control the transcription of defined subsets of genes in various cell systems. In this study we examined the role of BRD4 in promoting lineage-specific gene expression and show that BRD4 is essential for osteoblast differentiation. Genome-wide analyses demonstrate that BRD4 is recruited to the transcriptional start site of differentiation-induced genes. Unexpectedly, while promoter-proximal BRD4 occupancy correlated with gene expression, genes which displayed moderate expression and promoter-proximal BRD4 occupancy were most highly regulated and sensitive to BRD4 inhibition. Therefore, we examined distal BRD4 occupancy and uncovered a specific co-localization of BRD4 with the transcription factors C/EBPb, TEAD1, FOSL2 and JUND at putative osteoblast-specific enhancers. These findings reveal the intricacies of lineage specification and provide new insight into the context-dependent functions of BRD4.

AB - Proper temporal epigenetic regulation of gene expression is essential for cell fate determination and tissue development. The Bromodomain-containing Protein-4 (BRD4) was previously shown to control the transcription of defined subsets of genes in various cell systems. In this study we examined the role of BRD4 in promoting lineage-specific gene expression and show that BRD4 is essential for osteoblast differentiation. Genome-wide analyses demonstrate that BRD4 is recruited to the transcriptional start site of differentiation-induced genes. Unexpectedly, while promoter-proximal BRD4 occupancy correlated with gene expression, genes which displayed moderate expression and promoter-proximal BRD4 occupancy were most highly regulated and sensitive to BRD4 inhibition. Therefore, we examined distal BRD4 occupancy and uncovered a specific co-localization of BRD4 with the transcription factors C/EBPb, TEAD1, FOSL2 and JUND at putative osteoblast-specific enhancers. These findings reveal the intricacies of lineage specification and provide new insight into the context-dependent functions of BRD4.

U2 - 10.1093/nar/gkw826

DO - 10.1093/nar/gkw826

M3 - SCORING: Journal article

C2 - 27651452

VL - 45

SP - 127

EP - 141

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 1

ER -