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BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice.

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BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice. / Spiro, Alexander Simon; Beil, Frank Timo; Baranowsky, Anke; Barvencik, Florian; Schilling, Arndt; Nguyen, Khoa; Khadem, Shahram; Seitz, Sebastian; Rueger, Johannes Maria; Schinke, Thorsten; Amling, Michael.

In: J ORTHOP RES, Vol. 28, No. 6, 6, 2010, p. 785-791.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Spiro, AS, Beil, FT, Baranowsky, A, Barvencik, F, Schilling, A, Nguyen, K, Khadem, S, Seitz, S, Rueger, JM, Schinke, T & Amling, M 2010, 'BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice.', J ORTHOP RES, vol. 28, no. 6, 6, pp. 785-791.

APA

Spiro, A. S., Beil, F. T., Baranowsky, A., Barvencik, F., Schilling, A., Nguyen, K., Khadem, S., Seitz, S., Rueger, J. M., Schinke, T., & Amling, M. (2010). BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice. J ORTHOP RES, 28(6), 785-791. [6].

Vancouver

Spiro AS, Beil FT, Baranowsky A, Barvencik F, Schilling A, Nguyen K et al. BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice. J ORTHOP RES. 2010;28(6):785-791. 6.

Bibtex

@article{1cf48ab4a1f34c669665c736e2bbaf40,
title = "BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice.",
abstract = "Nonsteroidal antiinflammatory drugs (NSAIDs) are known to potentially impair the fracture healing process. The aim of the present study was to determine if the impairment of bone healing by systemic NSAID application is, at least in part, due to an interaction of NSAIDs with the bone anabolic BMP-7 pathway. Therefore, we first analyzed fracture healing in control and diclofenac-treated mice, where we not only found a significant impairment of fracture healing due to diclofenac treatment as assessed by biomechanical testing and microCT imaging, but also found high coexpression of bone morphogenetic protein-7 (BMP-7) and cyclooxygenase-2 (COX-2) within the fracture callus of both groups. To experimentally address the possible interaction between BMP-7 and COX-2, we then induced ectopic bone formation in control (n = 10) and diclofenac-treated mice (n = 10) by application of BMP-7 (recombinant human OP-1, rhOP-1) into the hamstring muscles. After 20 days of treatment, each ectopic bone nodule was analyzed by contact-radiography, microCT, histology, and histomorphometry. Diclofenac application decreased the trabecular number and bone mass in the ectopic bone nodules significantly due to reduced osteoblast number and activity. These data demonstrate that the bone anabolic effect of BMP-7 and fracture healing is impaired by diclofenac application, and suggest that the potential negative impact of NSAIDs on fracture healing is, at least in part, due to interference with BMP-7 signaling. (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.",
author = "Spiro, {Alexander Simon} and Beil, {Frank Timo} and Anke Baranowsky and Florian Barvencik and Arndt Schilling and Khoa Nguyen and Shahram Khadem and Sebastian Seitz and Rueger, {Johannes Maria} and Thorsten Schinke and Michael Amling",
year = "2010",
language = "Deutsch",
volume = "28",
pages = "785--791",
journal = "J ORTHOP RES",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - BMP-7-induced ectopic bone formation and fracture healing is impaired by systemic NSAID application in C57BL/6-mice.

AU - Spiro, Alexander Simon

AU - Beil, Frank Timo

AU - Baranowsky, Anke

AU - Barvencik, Florian

AU - Schilling, Arndt

AU - Nguyen, Khoa

AU - Khadem, Shahram

AU - Seitz, Sebastian

AU - Rueger, Johannes Maria

AU - Schinke, Thorsten

AU - Amling, Michael

PY - 2010

Y1 - 2010

N2 - Nonsteroidal antiinflammatory drugs (NSAIDs) are known to potentially impair the fracture healing process. The aim of the present study was to determine if the impairment of bone healing by systemic NSAID application is, at least in part, due to an interaction of NSAIDs with the bone anabolic BMP-7 pathway. Therefore, we first analyzed fracture healing in control and diclofenac-treated mice, where we not only found a significant impairment of fracture healing due to diclofenac treatment as assessed by biomechanical testing and microCT imaging, but also found high coexpression of bone morphogenetic protein-7 (BMP-7) and cyclooxygenase-2 (COX-2) within the fracture callus of both groups. To experimentally address the possible interaction between BMP-7 and COX-2, we then induced ectopic bone formation in control (n = 10) and diclofenac-treated mice (n = 10) by application of BMP-7 (recombinant human OP-1, rhOP-1) into the hamstring muscles. After 20 days of treatment, each ectopic bone nodule was analyzed by contact-radiography, microCT, histology, and histomorphometry. Diclofenac application decreased the trabecular number and bone mass in the ectopic bone nodules significantly due to reduced osteoblast number and activity. These data demonstrate that the bone anabolic effect of BMP-7 and fracture healing is impaired by diclofenac application, and suggest that the potential negative impact of NSAIDs on fracture healing is, at least in part, due to interference with BMP-7 signaling. (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

AB - Nonsteroidal antiinflammatory drugs (NSAIDs) are known to potentially impair the fracture healing process. The aim of the present study was to determine if the impairment of bone healing by systemic NSAID application is, at least in part, due to an interaction of NSAIDs with the bone anabolic BMP-7 pathway. Therefore, we first analyzed fracture healing in control and diclofenac-treated mice, where we not only found a significant impairment of fracture healing due to diclofenac treatment as assessed by biomechanical testing and microCT imaging, but also found high coexpression of bone morphogenetic protein-7 (BMP-7) and cyclooxygenase-2 (COX-2) within the fracture callus of both groups. To experimentally address the possible interaction between BMP-7 and COX-2, we then induced ectopic bone formation in control (n = 10) and diclofenac-treated mice (n = 10) by application of BMP-7 (recombinant human OP-1, rhOP-1) into the hamstring muscles. After 20 days of treatment, each ectopic bone nodule was analyzed by contact-radiography, microCT, histology, and histomorphometry. Diclofenac application decreased the trabecular number and bone mass in the ectopic bone nodules significantly due to reduced osteoblast number and activity. These data demonstrate that the bone anabolic effect of BMP-7 and fracture healing is impaired by diclofenac application, and suggest that the potential negative impact of NSAIDs on fracture healing is, at least in part, due to interference with BMP-7 signaling. (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

M3 - SCORING: Zeitschriftenaufsatz

VL - 28

SP - 785

EP - 791

JO - J ORTHOP RES

JF - J ORTHOP RES

SN - 0736-0266

IS - 6

M1 - 6

ER -