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Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model

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Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model. / Pautke, Christoph; Kreutzer, Kilian; Weitz, Jochen; Knödler, Martina; Münzel, Daniela; Wexel, Gabriele; Otto, Sven; Hapfelmeier, Alexander; Stürzenbaum, Stephen; Tischer, Thomas.

In: BONE, Vol. 51, No. 3, 09.2012, p. 592-9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Pautke, C, Kreutzer, K, Weitz, J, Knödler, M, Münzel, D, Wexel, G, Otto, S, Hapfelmeier, A, Stürzenbaum, S & Tischer, T 2012, 'Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model', BONE, vol. 51, no. 3, pp. 592-9. https://doi.org/10.1016/j.bone.2012.04.020

APA

Pautke, C., Kreutzer, K., Weitz, J., Knödler, M., Münzel, D., Wexel, G., Otto, S., Hapfelmeier, A., Stürzenbaum, S., & Tischer, T. (2012). Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model. BONE, 51(3), 592-9. https://doi.org/10.1016/j.bone.2012.04.020

Vancouver

Pautke C, Kreutzer K, Weitz J, Knödler M, Münzel D, Wexel G et al. Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model. BONE. 2012 Sep;51(3):592-9. https://doi.org/10.1016/j.bone.2012.04.020

Bibtex

@article{51441c2fe4464263a5c991736af46f38,
title = "Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model",
abstract = "Bisphosphonate related osteonecrosis of the jaw (BRONJ) is rare but potentially severe, and the etiopathology and risk factors are poorly defined. To date, it has not been possible to induce BRONJ in a large animal model, a shortfall this study aims to redress. Ten two-year-old adult G{\"o}ttingen minipigs were split into two groups. Five pigs (group 1) were administered intravenously a weekly dose of a bisphosphonate (zoledonate 0.05mg/kg body weight, approximating the oncologic dose in humans) and five pigs (group 2) served as controls. After 6 weeks, tooth extractions were performed in the upper and lower jaw (both groups) and the bisphosphonate administration continued for a further 10 weeks (group 1 only). Clinical and blood parameters were monitored throughout the entire experiment; thereafter, the jaw-bones were subjected to macroscopic, radiological (CT) and histological investigations. Whilst the extraction sites in the control group healed within two weeks, all animals in the bisphosphonate group exhibited exposed bone and impaired wound healing, indicators that are synonymous of macroscopically advanced osteonecrosis. Radiological and in particular histological investigations confirmed the presence of BRONJ in the animals from group 1. This paper demonstrates that the administration of bisphosphonates, in combination with tooth extractions, induces BRONJ in a minipig model. The ability to study BRONJ in miniature pigs, animals with a bone structure not dissimilar to humans, may improve our knowledgebase regarding the etiopathology, the prophylaxis and potentially uncover new therapies of BRONJ.",
keywords = "Animals, Bisphosphonate-Associated Osteonecrosis of the Jaw, Disease Models, Animal, Microscopy, Fluorescence, Paraffin Embedding, Swine, Swine, Miniature, Tomography, X-Ray Computed, Tooth Extraction",
author = "Christoph Pautke and Kilian Kreutzer and Jochen Weitz and Martina Kn{\"o}dler and Daniela M{\"u}nzel and Gabriele Wexel and Sven Otto and Alexander Hapfelmeier and Stephen St{\"u}rzenbaum and Thomas Tischer",
note = "Crown Copyright {\textcopyright} 2012. Published by Elsevier Inc. All rights reserved.",
year = "2012",
month = sep,
doi = "10.1016/j.bone.2012.04.020",
language = "English",
volume = "51",
pages = "592--9",
journal = "BONE",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Bisphosphonate related osteonecrosis of the jaw: A minipig large animal model

AU - Pautke, Christoph

AU - Kreutzer, Kilian

AU - Weitz, Jochen

AU - Knödler, Martina

AU - Münzel, Daniela

AU - Wexel, Gabriele

AU - Otto, Sven

AU - Hapfelmeier, Alexander

AU - Stürzenbaum, Stephen

AU - Tischer, Thomas

N1 - Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

PY - 2012/9

Y1 - 2012/9

N2 - Bisphosphonate related osteonecrosis of the jaw (BRONJ) is rare but potentially severe, and the etiopathology and risk factors are poorly defined. To date, it has not been possible to induce BRONJ in a large animal model, a shortfall this study aims to redress. Ten two-year-old adult Göttingen minipigs were split into two groups. Five pigs (group 1) were administered intravenously a weekly dose of a bisphosphonate (zoledonate 0.05mg/kg body weight, approximating the oncologic dose in humans) and five pigs (group 2) served as controls. After 6 weeks, tooth extractions were performed in the upper and lower jaw (both groups) and the bisphosphonate administration continued for a further 10 weeks (group 1 only). Clinical and blood parameters were monitored throughout the entire experiment; thereafter, the jaw-bones were subjected to macroscopic, radiological (CT) and histological investigations. Whilst the extraction sites in the control group healed within two weeks, all animals in the bisphosphonate group exhibited exposed bone and impaired wound healing, indicators that are synonymous of macroscopically advanced osteonecrosis. Radiological and in particular histological investigations confirmed the presence of BRONJ in the animals from group 1. This paper demonstrates that the administration of bisphosphonates, in combination with tooth extractions, induces BRONJ in a minipig model. The ability to study BRONJ in miniature pigs, animals with a bone structure not dissimilar to humans, may improve our knowledgebase regarding the etiopathology, the prophylaxis and potentially uncover new therapies of BRONJ.

AB - Bisphosphonate related osteonecrosis of the jaw (BRONJ) is rare but potentially severe, and the etiopathology and risk factors are poorly defined. To date, it has not been possible to induce BRONJ in a large animal model, a shortfall this study aims to redress. Ten two-year-old adult Göttingen minipigs were split into two groups. Five pigs (group 1) were administered intravenously a weekly dose of a bisphosphonate (zoledonate 0.05mg/kg body weight, approximating the oncologic dose in humans) and five pigs (group 2) served as controls. After 6 weeks, tooth extractions were performed in the upper and lower jaw (both groups) and the bisphosphonate administration continued for a further 10 weeks (group 1 only). Clinical and blood parameters were monitored throughout the entire experiment; thereafter, the jaw-bones were subjected to macroscopic, radiological (CT) and histological investigations. Whilst the extraction sites in the control group healed within two weeks, all animals in the bisphosphonate group exhibited exposed bone and impaired wound healing, indicators that are synonymous of macroscopically advanced osteonecrosis. Radiological and in particular histological investigations confirmed the presence of BRONJ in the animals from group 1. This paper demonstrates that the administration of bisphosphonates, in combination with tooth extractions, induces BRONJ in a minipig model. The ability to study BRONJ in miniature pigs, animals with a bone structure not dissimilar to humans, may improve our knowledgebase regarding the etiopathology, the prophylaxis and potentially uncover new therapies of BRONJ.

KW - Animals

KW - Bisphosphonate-Associated Osteonecrosis of the Jaw

KW - Disease Models, Animal

KW - Microscopy, Fluorescence

KW - Paraffin Embedding

KW - Swine

KW - Swine, Miniature

KW - Tomography, X-Ray Computed

KW - Tooth Extraction

U2 - 10.1016/j.bone.2012.04.020

DO - 10.1016/j.bone.2012.04.020

M3 - SCORING: Journal article

C2 - 22575441

VL - 51

SP - 592

EP - 599

JO - BONE

JF - BONE

SN - 8756-3282

IS - 3

ER -