BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons
Standard
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons. / Vierock, Johannes; Rodriguez-Rozada, Silvia; Dieter, Alexander; Pieper, Florian; Sims, Ruth; Tenedini, Federico; Bergs, Amelie C F; Bendifallah, Imane; Zhou, Fangmin; Zeitzschel, Nadja; Ahlbeck, Joachim; Augustin, Sandra; Sauter, Kathrin; Papagiakoumou, Eirini; Gottschalk, Alexander; Soba, Peter; Emiliani, Valentina; Engel, Andreas K; Hegemann, Peter; Wiegert, J Simon.
In: NAT COMMUN, Vol. 12, No. 1, 26.07.2021, p. 4527.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons
AU - Vierock, Johannes
AU - Rodriguez-Rozada, Silvia
AU - Dieter, Alexander
AU - Pieper, Florian
AU - Sims, Ruth
AU - Tenedini, Federico
AU - Bergs, Amelie C F
AU - Bendifallah, Imane
AU - Zhou, Fangmin
AU - Zeitzschel, Nadja
AU - Ahlbeck, Joachim
AU - Augustin, Sandra
AU - Sauter, Kathrin
AU - Papagiakoumou, Eirini
AU - Gottschalk, Alexander
AU - Soba, Peter
AU - Emiliani, Valentina
AU - Engel, Andreas K
AU - Hegemann, Peter
AU - Wiegert, J Simon
N1 - © 2021. The Author(s).
PY - 2021/7/26
Y1 - 2021/7/26
N2 - Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.
AB - Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.
U2 - 10.1038/s41467-021-24759-5
DO - 10.1038/s41467-021-24759-5
M3 - SCORING: Journal article
C2 - 34312384
VL - 12
SP - 4527
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
ER -
