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Bioreactor cultivation of three-dimensional cartilage-carrier-constructs.

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Bioreactor cultivation of three-dimensional cartilage-carrier-constructs. / Nagel-Heyer, Stephanie; Goepfert, Christiane; Feyerabend, Frank; Petersen, Jan Philipp; Adamietz, Peter; Meenen, Norbert; Pörtner, Ralf.

In: BIOPROC BIOSYST ENG, Vol. 27, No. 4, 4, 2005, p. 273-280.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Nagel-Heyer, S, Goepfert, C, Feyerabend, F, Petersen, JP, Adamietz, P, Meenen, N & Pörtner, R 2005, 'Bioreactor cultivation of three-dimensional cartilage-carrier-constructs.', BIOPROC BIOSYST ENG, vol. 27, no. 4, 4, pp. 273-280. <http://www.ncbi.nlm.nih.gov/pubmed/15928929?dopt=Citation>

APA

Nagel-Heyer, S., Goepfert, C., Feyerabend, F., Petersen, J. P., Adamietz, P., Meenen, N., & Pörtner, R. (2005). Bioreactor cultivation of three-dimensional cartilage-carrier-constructs. BIOPROC BIOSYST ENG, 27(4), 273-280. [4]. http://www.ncbi.nlm.nih.gov/pubmed/15928929?dopt=Citation

Vancouver

Nagel-Heyer S, Goepfert C, Feyerabend F, Petersen JP, Adamietz P, Meenen N et al. Bioreactor cultivation of three-dimensional cartilage-carrier-constructs. BIOPROC BIOSYST ENG. 2005;27(4):273-280. 4.

Bibtex

@article{405c09498ec04098b24be1c6f98a2787,
title = "Bioreactor cultivation of three-dimensional cartilage-carrier-constructs.",
abstract = "A flow-chamber bioreactor was designed for generation of three-dimensional cartilage-carrier-constructs. A specific attribute of the flow-chamber is a very thin medium layer for improved oxygen supply and a counter current flow of medium and gas. Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. The final step of this protocol was performed either in the bioreactor or in 12-well plates. The bioreactor experiments showed a significantly higher matrix thickness but a lower ratio of glycosaminoglycan to DNA. For both culture methods the constructs contained a high amount of collagen II. Appearance of the cartilage obtained in the bioreactor seemed to be closer to native cartilage with respect to distribution of the cells within the matrix, smoothness of the surface etc. All results considered the flow-chamber bioreactor is a very useful tool for generation of three dimensional cartilage-carrier constructs.",
keywords = "Cells, Cultured, *Bioreactors, *Tissue Engineering/methods, *Cartilage/physiology, Chondrocytes/cytology/*physiology, Cells, Cultured, *Bioreactors, *Tissue Engineering/methods, *Cartilage/physiology, Chondrocytes/cytology/*physiology",
author = "Stephanie Nagel-Heyer and Christiane Goepfert and Frank Feyerabend and Petersen, {Jan Philipp} and Peter Adamietz and Norbert Meenen and Ralf P{\"o}rtner",
year = "2005",
language = "English",
volume = "27",
pages = "273--280",
journal = "BIOPROC BIOSYST ENG",
issn = "1615-7591",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Bioreactor cultivation of three-dimensional cartilage-carrier-constructs.

AU - Nagel-Heyer, Stephanie

AU - Goepfert, Christiane

AU - Feyerabend, Frank

AU - Petersen, Jan Philipp

AU - Adamietz, Peter

AU - Meenen, Norbert

AU - Pörtner, Ralf

PY - 2005

Y1 - 2005

N2 - A flow-chamber bioreactor was designed for generation of three-dimensional cartilage-carrier-constructs. A specific attribute of the flow-chamber is a very thin medium layer for improved oxygen supply and a counter current flow of medium and gas. Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. The final step of this protocol was performed either in the bioreactor or in 12-well plates. The bioreactor experiments showed a significantly higher matrix thickness but a lower ratio of glycosaminoglycan to DNA. For both culture methods the constructs contained a high amount of collagen II. Appearance of the cartilage obtained in the bioreactor seemed to be closer to native cartilage with respect to distribution of the cells within the matrix, smoothness of the surface etc. All results considered the flow-chamber bioreactor is a very useful tool for generation of three dimensional cartilage-carrier constructs.

AB - A flow-chamber bioreactor was designed for generation of three-dimensional cartilage-carrier-constructs. A specific attribute of the flow-chamber is a very thin medium layer for improved oxygen supply and a counter current flow of medium and gas. Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. The final step of this protocol was performed either in the bioreactor or in 12-well plates. The bioreactor experiments showed a significantly higher matrix thickness but a lower ratio of glycosaminoglycan to DNA. For both culture methods the constructs contained a high amount of collagen II. Appearance of the cartilage obtained in the bioreactor seemed to be closer to native cartilage with respect to distribution of the cells within the matrix, smoothness of the surface etc. All results considered the flow-chamber bioreactor is a very useful tool for generation of three dimensional cartilage-carrier constructs.

KW - Cells, Cultured

KW - Bioreactors

KW - Tissue Engineering/methods

KW - Cartilage/physiology

KW - Chondrocytes/cytology/physiology

KW - Cells, Cultured

KW - Bioreactors

KW - Tissue Engineering/methods

KW - Cartilage/physiology

KW - Chondrocytes/cytology/physiology

M3 - SCORING: Journal article

VL - 27

SP - 273

EP - 280

JO - BIOPROC BIOSYST ENG

JF - BIOPROC BIOSYST ENG

SN - 1615-7591

IS - 4

M1 - 4

ER -