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Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples

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Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples. / Harris, Llinos G; Murray, Susan; Pascoe, Ben; Bray, James; Meric, Guillaume; Magerios, Leonardos; Wilkinson, Thomas S; Jeeves, Rose; Rohde, Holger; Schwarz, Stefan; de Lencastre, Herminia; Miragaia, Maria; Rolo, Joana; Bowden, Rory; Jolley, Keith A; Maiden, Martin C J; Mack, Dietrich; Sheppard, Samuel K.

In: PLOS ONE, Vol. 11, No. 3, 2016, p. e0151240.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Harris, LG, Murray, S, Pascoe, B, Bray, J, Meric, G, Magerios, L, Wilkinson, TS, Jeeves, R, Rohde, H, Schwarz, S, de Lencastre, H, Miragaia, M, Rolo, J, Bowden, R, Jolley, KA, Maiden, MCJ, Mack, D & Sheppard, SK 2016, 'Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples', PLOS ONE, vol. 11, no. 3, pp. e0151240. https://doi.org/10.1371/journal.pone.0151240

APA

Harris, L. G., Murray, S., Pascoe, B., Bray, J., Meric, G., Magerios, L., Wilkinson, T. S., Jeeves, R., Rohde, H., Schwarz, S., de Lencastre, H., Miragaia, M., Rolo, J., Bowden, R., Jolley, K. A., Maiden, M. C. J., Mack, D., & Sheppard, S. K. (2016). Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples. PLOS ONE, 11(3), e0151240. https://doi.org/10.1371/journal.pone.0151240

Vancouver

Harris LG, Murray S, Pascoe B, Bray J, Meric G, Magerios L et al. Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples. PLOS ONE. 2016;11(3):e0151240. https://doi.org/10.1371/journal.pone.0151240

Bibtex

@article{d827ada01f244df59324a1d353ff1b0b,
title = "Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples",
abstract = "Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.",
author = "Harris, {Llinos G} and Susan Murray and Ben Pascoe and James Bray and Guillaume Meric and Leonardos Magerios and Wilkinson, {Thomas S} and Rose Jeeves and Holger Rohde and Stefan Schwarz and {de Lencastre}, Herminia and Maria Miragaia and Joana Rolo and Rory Bowden and Jolley, {Keith A} and Maiden, {Martin C J} and Dietrich Mack and Sheppard, {Samuel K}",
year = "2016",
doi = "10.1371/journal.pone.0151240",
language = "English",
volume = "11",
pages = "e0151240",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Biofilm Morphotypes and Population Structure among Staphylococcus epidermidis from Commensal and Clinical Samples

AU - Harris, Llinos G

AU - Murray, Susan

AU - Pascoe, Ben

AU - Bray, James

AU - Meric, Guillaume

AU - Magerios, Leonardos

AU - Wilkinson, Thomas S

AU - Jeeves, Rose

AU - Rohde, Holger

AU - Schwarz, Stefan

AU - de Lencastre, Herminia

AU - Miragaia, Maria

AU - Rolo, Joana

AU - Bowden, Rory

AU - Jolley, Keith A

AU - Maiden, Martin C J

AU - Mack, Dietrich

AU - Sheppard, Samuel K

PY - 2016

Y1 - 2016

N2 - Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.

AB - Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.

U2 - 10.1371/journal.pone.0151240

DO - 10.1371/journal.pone.0151240

M3 - SCORING: Journal article

C2 - 26978068

VL - 11

SP - e0151240

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 3

ER -