Binding inhibition of type 1 fimbriae to human granulocytes: a flow cytometric inhibition assay using trivalent cluster mannosides.
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Binding inhibition of type 1 fimbriae to human granulocytes: a flow cytometric inhibition assay using trivalent cluster mannosides. / Horst, Andrea; Kötter, S; Lindhorst, T K; Ludwig, A; Brandt, E; Wagener, C.
In: MED MICROBIOL IMMUN, Vol. 190, No. 3, 3, 2001, p. 145-149.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Binding inhibition of type 1 fimbriae to human granulocytes: a flow cytometric inhibition assay using trivalent cluster mannosides.
AU - Horst, Andrea
AU - Kötter, S
AU - Lindhorst, T K
AU - Ludwig, A
AU - Brandt, E
AU - Wagener, C
PY - 2001
Y1 - 2001
N2 - The binding of type 1 fimbriae from Escherichia coli to vital human neutrophilic granulocytes was inhibited by synthetic trivalent cluster mannosides. Binding of type 1 fimbriae was measured in a flow cytometric assay. Based on the molarity of mannosyl residues, the clusters exceed the inhibitory potency of methyl alpha-D-mannoside by a factor of more than 1,000 and the inhibitory potency of p-nitrophenyl alpha-D-mannoside by a factor of more than 10. The inhibition studies indicate that the trivalent cluster mannosides are very potent inhibitors of the binding of type 1 fimbriae to human neutrophilic granulocytes. Based on their defined structure, cluster mannosides are well suited for characterizing the molecular interactions of mannose-sensitive fimbriae with their cell membrane receptors.
AB - The binding of type 1 fimbriae from Escherichia coli to vital human neutrophilic granulocytes was inhibited by synthetic trivalent cluster mannosides. Binding of type 1 fimbriae was measured in a flow cytometric assay. Based on the molarity of mannosyl residues, the clusters exceed the inhibitory potency of methyl alpha-D-mannoside by a factor of more than 1,000 and the inhibitory potency of p-nitrophenyl alpha-D-mannoside by a factor of more than 10. The inhibition studies indicate that the trivalent cluster mannosides are very potent inhibitors of the binding of type 1 fimbriae to human neutrophilic granulocytes. Based on their defined structure, cluster mannosides are well suited for characterizing the molecular interactions of mannose-sensitive fimbriae with their cell membrane receptors.
M3 - SCORING: Zeitschriftenaufsatz
VL - 190
SP - 145
EP - 149
JO - MED MICROBIOL IMMUN
JF - MED MICROBIOL IMMUN
SN - 0300-8584
IS - 3
M1 - 3
ER -
