Bedside assessment of multiphoton tomography
Related Research units
Abstract
The optical biopsies generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes indicating an altered cellular metabolism. Morphometric algorithms reliably showed a perinuclear accumulation in lesional skin in contrast to an even distribution in healthy skin. MPT-FLIM confirms these observations showing a metabolic shift in lesions. Moreover, detection of the onset and progression of inflammatory processes could be achieved.
In conclusion, the change in the distribution of mitochondria and the verification of an altered cellular metabolism facilitate a better understanding of the processes of wound healing and inflammatory skin diseases envisioning future clinical areas of MPT-FLIM application.
Bibliographical data
Original language | English |
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Title of host publication | Multiphoton Microscopy and Fluorescence Lifetime Imaging : Applications in Biology and Medicine |
Editors | Karsten König |
REQUIRED books only: Number of pages | 20 |
Publisher | De Gruyter |
Publication date | 2018 |
Edition | 1 |
Pages | 425-444 |
Article number | 22 |
ISBN (Print) | 978-3110438987 |
ISBN (Electronic) | 9783110429985, 9783110430073 |
Publication status | Published - 2018 |