Balance between neurogenesis and gliogenesis in the adult hippocampus: role for reelin.
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Balance between neurogenesis and gliogenesis in the adult hippocampus: role for reelin. / Zhao, Shanting; Chai, Xuejun; Frotscher, Michael.
In: DEV NEUROSCI-BASEL, Vol. 29, No. 1-2, 1-2, 2007, p. 84-90.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Balance between neurogenesis and gliogenesis in the adult hippocampus: role for reelin.
AU - Zhao, Shanting
AU - Chai, Xuejun
AU - Frotscher, Michael
PY - 2007
Y1 - 2007
N2 - The extracellular matrix protein reelin is essential for the proper radial migration of cortical neurons. In reeler mice lacking reelin, there is a malformation of the radial glial scaffold required for granule cell migration. Immunostaining for glial fibrillary acidic protein (GFAP) reveals abundant radial glial cells with long fibers traversing the granular layer in the wild type, but almost exclusively astrocytes in the reeler mutant. With the concept that radial glial cells are precursors of neurons, we hypothesized that the balance between neurogenesis and gliogenesis is altered in the reeler mutant. To this end, adult reeler mutants and their wild-type littermates were injected with bromodeoxyuridine (BrdU), a marker of newly generated cells. When compared to wild-type animals, we found a reduction in the number of BrdU-labeled cells in the adult reeler dentate gyrus. Moreover, whereas there was a dramatic decrease in the number of newly generated granule cells identified by double labeling for BrdU and NeuN, the number of BrdU-labeled, GFAP-positive astrocytes had increased. Decreased neurogenesis in the adult reeler dentate gyrus was confirmed by immunostaining for doublecortin, a marker of newly generated neurons. These results indicate that adult neurogenesis is altered in the reeler dentate gyrus and that newly generated cells preferentially differentiate into astrocytes.
AB - The extracellular matrix protein reelin is essential for the proper radial migration of cortical neurons. In reeler mice lacking reelin, there is a malformation of the radial glial scaffold required for granule cell migration. Immunostaining for glial fibrillary acidic protein (GFAP) reveals abundant radial glial cells with long fibers traversing the granular layer in the wild type, but almost exclusively astrocytes in the reeler mutant. With the concept that radial glial cells are precursors of neurons, we hypothesized that the balance between neurogenesis and gliogenesis is altered in the reeler mutant. To this end, adult reeler mutants and their wild-type littermates were injected with bromodeoxyuridine (BrdU), a marker of newly generated cells. When compared to wild-type animals, we found a reduction in the number of BrdU-labeled cells in the adult reeler dentate gyrus. Moreover, whereas there was a dramatic decrease in the number of newly generated granule cells identified by double labeling for BrdU and NeuN, the number of BrdU-labeled, GFAP-positive astrocytes had increased. Decreased neurogenesis in the adult reeler dentate gyrus was confirmed by immunostaining for doublecortin, a marker of newly generated neurons. These results indicate that adult neurogenesis is altered in the reeler dentate gyrus and that newly generated cells preferentially differentiate into astrocytes.
KW - Animals
KW - Male
KW - Immunohistochemistry
KW - Mice
KW - Cell Differentiation genetics
KW - Hippocampus metabolism
KW - Aging physiology
KW - Astrocytes metabolism
KW - Biological Markers
KW - Bromodeoxyuridine
KW - Cell Proliferation
KW - Glial Fibrillary Acidic Protein metabolism
KW - Mice, Neurologic Mutants
KW - Microtubule-Associated Proteins metabolism
KW - Neuronal Plasticity genetics
KW - Neurons metabolism
KW - Neuropeptides metabolism
KW - Stem Cells metabolism
KW - Animals
KW - Male
KW - Immunohistochemistry
KW - Mice
KW - Cell Differentiation genetics
KW - Hippocampus metabolism
KW - Aging physiology
KW - Astrocytes metabolism
KW - Biological Markers
KW - Bromodeoxyuridine
KW - Cell Proliferation
KW - Glial Fibrillary Acidic Protein metabolism
KW - Mice, Neurologic Mutants
KW - Microtubule-Associated Proteins metabolism
KW - Neuronal Plasticity genetics
KW - Neurons metabolism
KW - Neuropeptides metabolism
KW - Stem Cells metabolism
M3 - SCORING: Zeitschriftenaufsatz
VL - 29
SP - 84
EP - 90
JO - DEV NEUROSCI-BASEL
JF - DEV NEUROSCI-BASEL
SN - 0378-5866
IS - 1-2
M1 - 1-2
ER -
