B cell analysis in SARS‐CoV‐2 versus malaria: Increased frequencies of plasmablasts and atypical memory B cells in COVID‐19
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B cell analysis in SARS‐CoV‐2 versus malaria: Increased frequencies of plasmablasts and atypical memory B cells in COVID‐19. / Wildner, Nils H; Ahmadi, Parimah; Schulte, Sophia; Brauneck, Franziska; Kohsar, Matin; Lütgehetmann, Marc; Beisel, Claudia; Addo, Marylyn Martina; Haag, Friedrich; Schulze zur Wiesch, Julian.
In: J LEUKOCYTE BIOL, Vol. 109, No. 1, 01.2021, p. 77-90.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - B cell analysis in SARS‐CoV‐2 versus malaria: Increased frequencies of plasmablasts and atypical memory B cells in COVID‐19
AU - Wildner, Nils H
AU - Ahmadi, Parimah
AU - Schulte, Sophia
AU - Brauneck, Franziska
AU - Kohsar, Matin
AU - Lütgehetmann, Marc
AU - Beisel, Claudia
AU - Addo, Marylyn Martina
AU - Haag, Friedrich
AU - Schulze zur Wiesch, Julian
PY - 2021/1
Y1 - 2021/1
N2 - B cells play a central role in antiviral and antiparasitic immunity, not only as producers of antibod-ies, but also as APCs and mediators of inflammation. In this study, we used 16-color flow cytom-etry analysis to investigate the frequency, differentiation, and activation status of peripheral Bcells of patientswith SARS-CoV-2 infection or acute Plasmodium falciparum malaria compared withthe healthy individuals. As a main result, we observed an increase of the frequency of (CD27–,CD21–) atypical memoryB cells and (CD19+,CD27+,CD38+) plasmablasts inmalaria and COVID-19 patients. Additionally, CD86, PD-1, CXCR3, and CD39 expression was up-regulated, whereasCD73 was down-regulated on plasmablasts of COVID-19 and malaria patients compared withthe bulk B cell population. In particular, there was a more pronounced loss of CD73+B cells inmalaria. The frequency of plasmablasts positively correlated with serum levels of CRP, IL-6, andLDH of COVID-19 patients. In the longitudinal course of COVID-19, a rapid normalization of thefrequency of atypical memory B cells was observed. The role and function of plasmablasts andatypical memory B cells in COVID-19 and other acute infections remain to be further investigated.The role of B cells as either “driver or passenger” of hyperinflammation during COVID-19 needsto be clarified
AB - B cells play a central role in antiviral and antiparasitic immunity, not only as producers of antibod-ies, but also as APCs and mediators of inflammation. In this study, we used 16-color flow cytom-etry analysis to investigate the frequency, differentiation, and activation status of peripheral Bcells of patientswith SARS-CoV-2 infection or acute Plasmodium falciparum malaria compared withthe healthy individuals. As a main result, we observed an increase of the frequency of (CD27–,CD21–) atypical memoryB cells and (CD19+,CD27+,CD38+) plasmablasts inmalaria and COVID-19 patients. Additionally, CD86, PD-1, CXCR3, and CD39 expression was up-regulated, whereasCD73 was down-regulated on plasmablasts of COVID-19 and malaria patients compared withthe bulk B cell population. In particular, there was a more pronounced loss of CD73+B cells inmalaria. The frequency of plasmablasts positively correlated with serum levels of CRP, IL-6, andLDH of COVID-19 patients. In the longitudinal course of COVID-19, a rapid normalization of thefrequency of atypical memory B cells was observed. The role and function of plasmablasts andatypical memory B cells in COVID-19 and other acute infections remain to be further investigated.The role of B cells as either “driver or passenger” of hyperinflammation during COVID-19 needsto be clarified
UR - http://dx.doi.org/10.1002/jlb.5cova0620-370rr
U2 - 10.1002/jlb.5cova0620-370rr
DO - 10.1002/jlb.5cova0620-370rr
M3 - SCORING: Journal article
VL - 109
SP - 77
EP - 90
JO - J LEUKOCYTE BIOL
JF - J LEUKOCYTE BIOL
SN - 0741-5400
IS - 1
ER -
