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Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium

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Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium. / Lemme, Marta; Ulmer, Bärbel M; Lemoine, Marc D; Zech, Antonia T L; Flenner, Frederik; Ravens, Ursula; Reichenspurner, Hermann; Rol-Garcia, Miriam; Smith, Godfrey; Hansen, Arne; Christ, Torsten; Eschenhagen, Thomas.

In: STEM CELL REP, Vol. 11, No. 6, 11.12.2018, p. 1378-1390.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lemme, M, Ulmer, BM, Lemoine, MD, Zech, ATL, Flenner, F, Ravens, U, Reichenspurner, H, Rol-Garcia, M, Smith, G, Hansen, A, Christ, T & Eschenhagen, T 2018, 'Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium', STEM CELL REP, vol. 11, no. 6, pp. 1378-1390. https://doi.org/10.1016/j.stemcr.2018.10.008

APA

Lemme, M., Ulmer, B. M., Lemoine, M. D., Zech, A. T. L., Flenner, F., Ravens, U., Reichenspurner, H., Rol-Garcia, M., Smith, G., Hansen, A., Christ, T., & Eschenhagen, T. (2018). Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium. STEM CELL REP, 11(6), 1378-1390. https://doi.org/10.1016/j.stemcr.2018.10.008

Vancouver

Lemme M, Ulmer BM, Lemoine MD, Zech ATL, Flenner F, Ravens U et al. Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium. STEM CELL REP. 2018 Dec 11;11(6):1378-1390. https://doi.org/10.1016/j.stemcr.2018.10.008

Bibtex

@article{f44d492664294a779433b3970990f93e,
title = "Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium",
abstract = "Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs' behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening.",
keywords = "Journal Article",
author = "Marta Lemme and Ulmer, {B{\"a}rbel M} and Lemoine, {Marc D} and Zech, {Antonia T L} and Frederik Flenner and Ursula Ravens and Hermann Reichenspurner and Miriam Rol-Garcia and Godfrey Smith and Arne Hansen and Torsten Christ and Thomas Eschenhagen",
note = "Copyright {\textcopyright} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = dec,
day = "11",
doi = "10.1016/j.stemcr.2018.10.008",
language = "English",
volume = "11",
pages = "1378--1390",
journal = "STEM CELL REP",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium

AU - Lemme, Marta

AU - Ulmer, Bärbel M

AU - Lemoine, Marc D

AU - Zech, Antonia T L

AU - Flenner, Frederik

AU - Ravens, Ursula

AU - Reichenspurner, Hermann

AU - Rol-Garcia, Miriam

AU - Smith, Godfrey

AU - Hansen, Arne

AU - Christ, Torsten

AU - Eschenhagen, Thomas

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/12/11

Y1 - 2018/12/11

N2 - Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs' behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening.

AB - Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs' behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening.

KW - Journal Article

U2 - 10.1016/j.stemcr.2018.10.008

DO - 10.1016/j.stemcr.2018.10.008

M3 - SCORING: Journal article

C2 - 30416051

VL - 11

SP - 1378

EP - 1390

JO - STEM CELL REP

JF - STEM CELL REP

SN - 2213-6711

IS - 6

ER -