Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling.
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Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling. / Aicher, Alexandra; Brenner, Winfried; Zuhayra, Maaz; Badorff, Cornel; Schirin, Massoudi; Assmus, Birgit; Eckey, Thomas; Henze, Eberhard; Zeiher, Andreas M; Dimmeler, Stefanie.
In: CIRCULATION, Vol. 107, No. 16, 16, 2003, p. 2134-2139.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling.
AU - Aicher, Alexandra
AU - Brenner, Winfried
AU - Zuhayra, Maaz
AU - Badorff, Cornel
AU - Schirin, Massoudi
AU - Assmus, Birgit
AU - Eckey, Thomas
AU - Henze, Eberhard
AU - Zeiher, Andreas M
AU - Dimmeler, Stefanie
PY - 2003
Y1 - 2003
N2 - BACKGROUND: Transplantation of endothelial progenitor cells (EPCs) improves vascularization and left ventricular function after experimental myocardial ischemia. However, tissue distribution of transplanted EPCs has not yet been monitored in living animals. Therefore, we tested whether radioactive labeling allows us to detect injected EPCs. METHODS AND RESULTS: Human EPCs were isolated from peripheral blood, characterized by expression of endothelial marker proteins, and radioactively labeled with [111In]indium oxine. EPCs (106) were injected in athymic nude rats 24 hours after myocardial infarction (n=8) or sham operation (n=8). Scintigraphic images were acquired after 1, 24, 48, and 96 hours after EPC injection. Animals were then killed, and specific radioactivity was measured in different tissues. At 24 to 96 hours after intravenous injection of EPCs, approximately 70% of the radioactivity was localized in the spleen and liver, with only approximately 1% of the radioactivity identified in the heart of sham-operated animals. After myocardial infarction, the heart-to-muscle radioactivity ratio increased significantly, from 1.02+/-0.19 in sham-operated animals to 2.03+/-0.37 after intravenous administration of EPCs. Injection of EPCs into the left ventricular cavity increased this ratio profoundly, from 2.69+/-1.54 in sham-operated animals to 4.70+/-1.55 (P
AB - BACKGROUND: Transplantation of endothelial progenitor cells (EPCs) improves vascularization and left ventricular function after experimental myocardial ischemia. However, tissue distribution of transplanted EPCs has not yet been monitored in living animals. Therefore, we tested whether radioactive labeling allows us to detect injected EPCs. METHODS AND RESULTS: Human EPCs were isolated from peripheral blood, characterized by expression of endothelial marker proteins, and radioactively labeled with [111In]indium oxine. EPCs (106) were injected in athymic nude rats 24 hours after myocardial infarction (n=8) or sham operation (n=8). Scintigraphic images were acquired after 1, 24, 48, and 96 hours after EPC injection. Animals were then killed, and specific radioactivity was measured in different tissues. At 24 to 96 hours after intravenous injection of EPCs, approximately 70% of the radioactivity was localized in the spleen and liver, with only approximately 1% of the radioactivity identified in the heart of sham-operated animals. After myocardial infarction, the heart-to-muscle radioactivity ratio increased significantly, from 1.02+/-0.19 in sham-operated animals to 2.03+/-0.37 after intravenous administration of EPCs. Injection of EPCs into the left ventricular cavity increased this ratio profoundly, from 2.69+/-1.54 in sham-operated animals to 4.70+/-1.55 (P
M3 - SCORING: Zeitschriftenaufsatz
VL - 107
SP - 2134
EP - 2139
JO - CIRCULATION
JF - CIRCULATION
SN - 0009-7322
IS - 16
M1 - 16
ER -
