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Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach

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Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach. / Dunay, Gábor Artúr; Solomatina, Anastasia; Kummer, Silke; Hüfner, Anja; Bialek, Julia Katharina; Eberhard, Johanna Maria; Tolosa, Eva; Hauber, Joachim; Wiesch, Julian Schulze Zur.

In: VIRUS RES, Vol. 240, 15.08.2017, p. 107-111.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dunay, GA, Solomatina, A, Kummer, S, Hüfner, A, Bialek, JK, Eberhard, JM, Tolosa, E, Hauber, J & Wiesch, JSZ 2017, 'Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach', VIRUS RES, vol. 240, pp. 107-111. https://doi.org/10.1016/j.virusres.2017.07.008

APA

Dunay, G. A., Solomatina, A., Kummer, S., Hüfner, A., Bialek, J. K., Eberhard, J. M., Tolosa, E., Hauber, J., & Wiesch, J. S. Z. (2017). Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach. VIRUS RES, 240, 107-111. https://doi.org/10.1016/j.virusres.2017.07.008

Vancouver

Dunay GA, Solomatina A, Kummer S, Hüfner A, Bialek JK, Eberhard JM et al. Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach. VIRUS RES. 2017 Aug 15;240:107-111. https://doi.org/10.1016/j.virusres.2017.07.008

Bibtex

@article{9fbf0954eda149d5b54a4a73cf05b0f0,
title = "Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach",
abstract = "The relative contribution of regulatory T cells (Treg) as reservoir of HIV-1 in patients on chronic antiretroviral therapy is unclear to date. The aim of the current study was to assess the total HIV DNA burden and replication competent viral reservoir in Treg in comparison to central and effector memory cells (Tcm and Tem). Peripheral blood mononuclear cells were obtained from 10 HIV patients treated with antiretroviral therapy and one HIV elite controller. Droplet Digital PCR (ddPCR) was used to quantify total HIV DNA loads in FACS sorted CD4+ Treg (CD25+CD127lo) as compared with Tcm (CD45RO+CCR7+) and Tem (CD45RO+CCR7-). In contrast to earlier reports, no significant difference was found in total HIV DNA burden associated with Treg when compared to Tem and Tcm cells. In a subset of patients, quantitative viral outgrowth assays were also performed, using novel ddPCR based readout to quantify frequencies of Treg harboring replication competent virus.",
keywords = "Journal Article",
author = "Dunay, {G{\'a}bor Art{\'u}r} and Anastasia Solomatina and Silke Kummer and Anja H{\"u}fner and Bialek, {Julia Katharina} and Eberhard, {Johanna Maria} and Eva Tolosa and Joachim Hauber and Wiesch, {Julian Schulze Zur}",
note = "Copyright {\textcopyright} 2017. Published by Elsevier B.V.",
year = "2017",
month = aug,
day = "15",
doi = "10.1016/j.virusres.2017.07.008",
language = "English",
volume = "240",
pages = "107--111",
journal = "VIRUS RES",
issn = "0168-1702",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Assessment of the HIV-1 reservoir in CD4+ regulatory T cells by a Droplet Digital PCR based approach

AU - Dunay, Gábor Artúr

AU - Solomatina, Anastasia

AU - Kummer, Silke

AU - Hüfner, Anja

AU - Bialek, Julia Katharina

AU - Eberhard, Johanna Maria

AU - Tolosa, Eva

AU - Hauber, Joachim

AU - Wiesch, Julian Schulze Zur

N1 - Copyright © 2017. Published by Elsevier B.V.

PY - 2017/8/15

Y1 - 2017/8/15

N2 - The relative contribution of regulatory T cells (Treg) as reservoir of HIV-1 in patients on chronic antiretroviral therapy is unclear to date. The aim of the current study was to assess the total HIV DNA burden and replication competent viral reservoir in Treg in comparison to central and effector memory cells (Tcm and Tem). Peripheral blood mononuclear cells were obtained from 10 HIV patients treated with antiretroviral therapy and one HIV elite controller. Droplet Digital PCR (ddPCR) was used to quantify total HIV DNA loads in FACS sorted CD4+ Treg (CD25+CD127lo) as compared with Tcm (CD45RO+CCR7+) and Tem (CD45RO+CCR7-). In contrast to earlier reports, no significant difference was found in total HIV DNA burden associated with Treg when compared to Tem and Tcm cells. In a subset of patients, quantitative viral outgrowth assays were also performed, using novel ddPCR based readout to quantify frequencies of Treg harboring replication competent virus.

AB - The relative contribution of regulatory T cells (Treg) as reservoir of HIV-1 in patients on chronic antiretroviral therapy is unclear to date. The aim of the current study was to assess the total HIV DNA burden and replication competent viral reservoir in Treg in comparison to central and effector memory cells (Tcm and Tem). Peripheral blood mononuclear cells were obtained from 10 HIV patients treated with antiretroviral therapy and one HIV elite controller. Droplet Digital PCR (ddPCR) was used to quantify total HIV DNA loads in FACS sorted CD4+ Treg (CD25+CD127lo) as compared with Tcm (CD45RO+CCR7+) and Tem (CD45RO+CCR7-). In contrast to earlier reports, no significant difference was found in total HIV DNA burden associated with Treg when compared to Tem and Tcm cells. In a subset of patients, quantitative viral outgrowth assays were also performed, using novel ddPCR based readout to quantify frequencies of Treg harboring replication competent virus.

KW - Journal Article

U2 - 10.1016/j.virusres.2017.07.008

DO - 10.1016/j.virusres.2017.07.008

M3 - SCORING: Journal article

C2 - 28720421

VL - 240

SP - 107

EP - 111

JO - VIRUS RES

JF - VIRUS RES

SN - 0168-1702

ER -