Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon

TY - JOUR

T1 - Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon

AU - Lenz, Dominic

AU - Kremsner, Peter G

AU - Lell, Bertrand

AU - Biallas, Barbara

AU - Boettcher, Michael

AU - Mordmüller, Benjamin

AU - Adegnika, Ayola A

N1 - © 2011 Lenz et al; licensee BioMed Central Ltd.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - BACKGROUND: Rapid and accurate diagnosis of malaria is central to clinical management and the prevention of drug-overuse, which may lead to resistance development, toxicity and economic losses. So far, light microscopy (LM) of Giemsa-stained thick blood smears is the gold standard. Under optimal conditions the procedure is fast and reliable; nevertheless a gain in speed would be a great advantage. Rapid diagnosis tests are an alternative, although they cost more and give qualitative instead of quantitative results. Light-emitting diode (LED) fluorescence microscopy (ledFM 400 ×, 1000 ×) may offer a reliable and cheap alternative, which can be used at the point of care.METHODS: LedFM and conventional fluorescence microscopy (uvFM) were compared to LM in 210 samples from patients with history of fever in the last 24 hours admitted to the Albert Schweitzer Hospital in Lambaréné, Gabon.RESULTS: Sensitivities were 99.1% for ledFM and 97.0% for uvFM, specificities 90.7% for ledFM 400 × and 92.6% for ledFM 1000 × and uvFM. High agreement was found in Bland-Altman-plot and Kappa coefficient (ledFM 1000 ×: 0.914, ledFM 400 × and uvFM: 0.895). The time to diagnosis for both FM methods was shorter compared to LM (LM: 43 min, uvFM: 16 min, ledFM 1000 ×: 14 min, ledFM 400 ×: 10 min).CONCLUSION: ledFM is a reliable, accurate, fast and inexpensive tool for daily routine malaria diagnosis and may be used as a point of care diagnostic tool.

AB - BACKGROUND: Rapid and accurate diagnosis of malaria is central to clinical management and the prevention of drug-overuse, which may lead to resistance development, toxicity and economic losses. So far, light microscopy (LM) of Giemsa-stained thick blood smears is the gold standard. Under optimal conditions the procedure is fast and reliable; nevertheless a gain in speed would be a great advantage. Rapid diagnosis tests are an alternative, although they cost more and give qualitative instead of quantitative results. Light-emitting diode (LED) fluorescence microscopy (ledFM 400 ×, 1000 ×) may offer a reliable and cheap alternative, which can be used at the point of care.METHODS: LedFM and conventional fluorescence microscopy (uvFM) were compared to LM in 210 samples from patients with history of fever in the last 24 hours admitted to the Albert Schweitzer Hospital in Lambaréné, Gabon.RESULTS: Sensitivities were 99.1% for ledFM and 97.0% for uvFM, specificities 90.7% for ledFM 400 × and 92.6% for ledFM 1000 × and uvFM. High agreement was found in Bland-Altman-plot and Kappa coefficient (ledFM 1000 ×: 0.914, ledFM 400 × and uvFM: 0.895). The time to diagnosis for both FM methods was shorter compared to LM (LM: 43 min, uvFM: 16 min, ledFM 1000 ×: 14 min, ledFM 400 ×: 10 min).CONCLUSION: ledFM is a reliable, accurate, fast and inexpensive tool for daily routine malaria diagnosis and may be used as a point of care diagnostic tool.

KW - Gabon

KW - Humans

KW - Malaria, Falciparum

KW - Microscopy, Fluorescence

KW - Parasitology

KW - Plasmodium falciparum

KW - Point-of-Care Systems

KW - Sensitivity and Specificity

KW - Time Factors

U2 - 10.1186/1475-2875-10-194

DO - 10.1186/1475-2875-10-194

M3 - SCORING: Journal article

C2 - 21767366

VL - 10

SP - 194

JO - MALARIA J

JF - MALARIA J

SN - 1475-2875

ER -