Assessment of Immunological Biomarkers in the First Year after Heart Transplantation
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Assessment of Immunological Biomarkers in the First Year after Heart Transplantation. / Dieterlen, Maja-Theresa; John, Katja; Bittner, Hartmuth B; Mende, Meinhard; Tarnok, Attila; Mohr, Friedrich W; Barten, Markus J.
In: DIS MARKERS, Vol. 2015, 2015, p. 678061.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Assessment of Immunological Biomarkers in the First Year after Heart Transplantation
AU - Dieterlen, Maja-Theresa
AU - John, Katja
AU - Bittner, Hartmuth B
AU - Mende, Meinhard
AU - Tarnok, Attila
AU - Mohr, Friedrich W
AU - Barten, Markus J
PY - 2015
Y1 - 2015
N2 - BACKGROUND: Pharmacodynamic biomarkers that detect changes of immunological functions have been recognized as a helpful tool to increase the efficacy of immunosuppressive drug therapies. However, physiological changes of immunological biomarkers following transplantation are not investigated. Therefore, we assessed frequently used immunological biomarkers of the circulating blood in the first year following heart transplantation (HTx).METHODS: Activation markers CD25 and CD95, intracellular cytokines IL-2 and IFNγ, chemokines IP10 and MIG, and subsets of dendritic cells as well as antibodies against human leukocyte antigens (HLA) and major histocompatibility complex class I-related chain A (MICA) antigens were analyzed at different time points using flow cytometry and Luminex xMAP technology.RESULTS: Expression of IL-2, IFNγ, and plasmacytoid dendritic cells (pDCs) significantly increased (p < 0.01) during the first year. Anti-HLA antibodies decreased continuously, while anti-MICA antibodies showed minor increase within the first year. An association between percentage of pDCs and anti-MICA antibody positivity was proven. pDCs, IFNγ-producing T cells, and IP10 concentration were associated in a stronger way with age and gender of HTx recipients than with antibodies against HLA or MICA.CONCLUSIONS: We conclude that certain immunological biomarkers of the circulating blood change during the first year after HTx. These changes should be considered for interpretation of biomarkers after transplantation.
AB - BACKGROUND: Pharmacodynamic biomarkers that detect changes of immunological functions have been recognized as a helpful tool to increase the efficacy of immunosuppressive drug therapies. However, physiological changes of immunological biomarkers following transplantation are not investigated. Therefore, we assessed frequently used immunological biomarkers of the circulating blood in the first year following heart transplantation (HTx).METHODS: Activation markers CD25 and CD95, intracellular cytokines IL-2 and IFNγ, chemokines IP10 and MIG, and subsets of dendritic cells as well as antibodies against human leukocyte antigens (HLA) and major histocompatibility complex class I-related chain A (MICA) antigens were analyzed at different time points using flow cytometry and Luminex xMAP technology.RESULTS: Expression of IL-2, IFNγ, and plasmacytoid dendritic cells (pDCs) significantly increased (p < 0.01) during the first year. Anti-HLA antibodies decreased continuously, while anti-MICA antibodies showed minor increase within the first year. An association between percentage of pDCs and anti-MICA antibody positivity was proven. pDCs, IFNγ-producing T cells, and IP10 concentration were associated in a stronger way with age and gender of HTx recipients than with antibodies against HLA or MICA.CONCLUSIONS: We conclude that certain immunological biomarkers of the circulating blood change during the first year after HTx. These changes should be considered for interpretation of biomarkers after transplantation.
KW - Adult
KW - Biomarkers/blood
KW - Chemokines/blood
KW - Female
KW - Graft Rejection/blood
KW - Heart Transplantation/adverse effects
KW - Histocompatibility Antigens Class I/blood
KW - Humans
KW - Interleukin-2 Receptor alpha Subunit/blood
KW - Male
KW - Middle Aged
KW - fas Receptor/blood
U2 - 10.1155/2015/678061
DO - 10.1155/2015/678061
M3 - SCORING: Journal article
C2 - 26491215
VL - 2015
SP - 678061
JO - DIS MARKERS
JF - DIS MARKERS
SN - 0278-0240
ER -
