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Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein

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Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein. / Velagapudi, Srividya; Rohrer, Lucia; Poti, Francesco; Feuerborn, Renate; Perisa, Damir; Wang, Dongdong; Panteloglou, Grigorios; Potapenko, Anton; Yalcinkaya, Mustafa; Hülsmeier, Andreas J; Hesse, Bettina; Lukasz, Alexander; Liu, Mingxia; Parks, John S; Christoffersen, Christina; Stoffel, Markus; Simoni, Manuela; Nofer, Jerzy-Roch; von Eckardstein, Arnold.

In: ARTERIOSCL THROM VAS, Vol. 41, No. 10, 10.2021, p. e468-e479.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Velagapudi, S, Rohrer, L, Poti, F, Feuerborn, R, Perisa, D, Wang, D, Panteloglou, G, Potapenko, A, Yalcinkaya, M, Hülsmeier, AJ, Hesse, B, Lukasz, A, Liu, M, Parks, JS, Christoffersen, C, Stoffel, M, Simoni, M, Nofer, J-R & von Eckardstein, A 2021, 'Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein', ARTERIOSCL THROM VAS, vol. 41, no. 10, pp. e468-e479. https://doi.org/10.1161/ATVBAHA.121.316725

APA

Velagapudi, S., Rohrer, L., Poti, F., Feuerborn, R., Perisa, D., Wang, D., Panteloglou, G., Potapenko, A., Yalcinkaya, M., Hülsmeier, A. J., Hesse, B., Lukasz, A., Liu, M., Parks, J. S., Christoffersen, C., Stoffel, M., Simoni, M., Nofer, J-R., & von Eckardstein, A. (2021). Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein. ARTERIOSCL THROM VAS, 41(10), e468-e479. https://doi.org/10.1161/ATVBAHA.121.316725

Vancouver

Velagapudi S, Rohrer L, Poti F, Feuerborn R, Perisa D, Wang D et al. Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein. ARTERIOSCL THROM VAS. 2021 Oct;41(10):e468-e479. https://doi.org/10.1161/ATVBAHA.121.316725

Bibtex

@article{3ec6c0731cfe4559854c8e3a04f7d368,
title = "Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein",
abstract = "Objective:ApoM enriches S1P (sphingosine-1-phosphate) within HDL (high-density lipoproteins) and facilitates the activation of the S1P1 (S1P receptor type 1) by S1P, thereby preserving endothelial barrier function. Many protective functions exerted by HDL in extravascular tissues raise the question of how S1P regulates transendothelial HDL transport.Approach and Results:HDL were isolated from plasma of wild-type mice, Apom knockout mice, human apoM transgenic mice or humans and radioiodinated to trace its binding, association, and transport by bovine or human aortic endothelial cells. We also compared the transport of fluorescently-labeled HDL or Evans Blue, which labels albumin, from the tail vein into the peritoneal cavity of apoE-haploinsufficient mice with (apoE-haploinsufficient mice with endothelium-specific knockin of S1P1) or without (control mice, ie, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1) endothelium-specific knockin of S1P1. The binding, association, and transport of HDL from Apom knockout mice and human apoM-depleted HDL by bovine aortic endothelial cells was significantly lower than that of HDL from wild-type mice and human apoM-containing HDL, respectively. The binding, uptake, and transport of 125I-HDL by human aortic endothelial cells was increased by an S1P1 agonist but decreased by an S1P1 inhibitor. Silencing of SR-BI (scavenger receptor BI) abrogated the stimulation of 125I-HDL transport by the S1P1 agonist. Compared with control mice, that is, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1, apoE-haploinsufficient mice with endothelium-specific knockin of S1P1 showed decreased transport of Evans Blue but increased transport of HDL from blood into the peritoneal cavity and SR-BI expression in the aortal endothelium.Conclusions:ApoM and S1P1 promote transendothelial HDL transport. Their opposite effect on transendothelial transport of albumin and HDL indicates that HDL passes endothelial barriers by specific mechanisms rather than passive filtration.",
author = "Srividya Velagapudi and Lucia Rohrer and Francesco Poti and Renate Feuerborn and Damir Perisa and Dongdong Wang and Grigorios Panteloglou and Anton Potapenko and Mustafa Yalcinkaya and H{\"u}lsmeier, {Andreas J} and Bettina Hesse and Alexander Lukasz and Mingxia Liu and Parks, {John S} and Christina Christoffersen and Markus Stoffel and Manuela Simoni and Jerzy-Roch Nofer and {von Eckardstein}, Arnold",
year = "2021",
month = oct,
doi = "10.1161/ATVBAHA.121.316725",
language = "English",
volume = "41",
pages = "e468--e479",
journal = "ARTERIOSCL THROM VAS",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

RIS

TY - JOUR

T1 - Apolipoprotein M and Sphingosine-1-Phosphate Receptor 1 Promote the Transendothelial Transport of High-Density Lipoprotein

AU - Velagapudi, Srividya

AU - Rohrer, Lucia

AU - Poti, Francesco

AU - Feuerborn, Renate

AU - Perisa, Damir

AU - Wang, Dongdong

AU - Panteloglou, Grigorios

AU - Potapenko, Anton

AU - Yalcinkaya, Mustafa

AU - Hülsmeier, Andreas J

AU - Hesse, Bettina

AU - Lukasz, Alexander

AU - Liu, Mingxia

AU - Parks, John S

AU - Christoffersen, Christina

AU - Stoffel, Markus

AU - Simoni, Manuela

AU - Nofer, Jerzy-Roch

AU - von Eckardstein, Arnold

PY - 2021/10

Y1 - 2021/10

N2 - Objective:ApoM enriches S1P (sphingosine-1-phosphate) within HDL (high-density lipoproteins) and facilitates the activation of the S1P1 (S1P receptor type 1) by S1P, thereby preserving endothelial barrier function. Many protective functions exerted by HDL in extravascular tissues raise the question of how S1P regulates transendothelial HDL transport.Approach and Results:HDL were isolated from plasma of wild-type mice, Apom knockout mice, human apoM transgenic mice or humans and radioiodinated to trace its binding, association, and transport by bovine or human aortic endothelial cells. We also compared the transport of fluorescently-labeled HDL or Evans Blue, which labels albumin, from the tail vein into the peritoneal cavity of apoE-haploinsufficient mice with (apoE-haploinsufficient mice with endothelium-specific knockin of S1P1) or without (control mice, ie, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1) endothelium-specific knockin of S1P1. The binding, association, and transport of HDL from Apom knockout mice and human apoM-depleted HDL by bovine aortic endothelial cells was significantly lower than that of HDL from wild-type mice and human apoM-containing HDL, respectively. The binding, uptake, and transport of 125I-HDL by human aortic endothelial cells was increased by an S1P1 agonist but decreased by an S1P1 inhibitor. Silencing of SR-BI (scavenger receptor BI) abrogated the stimulation of 125I-HDL transport by the S1P1 agonist. Compared with control mice, that is, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1, apoE-haploinsufficient mice with endothelium-specific knockin of S1P1 showed decreased transport of Evans Blue but increased transport of HDL from blood into the peritoneal cavity and SR-BI expression in the aortal endothelium.Conclusions:ApoM and S1P1 promote transendothelial HDL transport. Their opposite effect on transendothelial transport of albumin and HDL indicates that HDL passes endothelial barriers by specific mechanisms rather than passive filtration.

AB - Objective:ApoM enriches S1P (sphingosine-1-phosphate) within HDL (high-density lipoproteins) and facilitates the activation of the S1P1 (S1P receptor type 1) by S1P, thereby preserving endothelial barrier function. Many protective functions exerted by HDL in extravascular tissues raise the question of how S1P regulates transendothelial HDL transport.Approach and Results:HDL were isolated from plasma of wild-type mice, Apom knockout mice, human apoM transgenic mice or humans and radioiodinated to trace its binding, association, and transport by bovine or human aortic endothelial cells. We also compared the transport of fluorescently-labeled HDL or Evans Blue, which labels albumin, from the tail vein into the peritoneal cavity of apoE-haploinsufficient mice with (apoE-haploinsufficient mice with endothelium-specific knockin of S1P1) or without (control mice, ie, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1) endothelium-specific knockin of S1P1. The binding, association, and transport of HDL from Apom knockout mice and human apoM-depleted HDL by bovine aortic endothelial cells was significantly lower than that of HDL from wild-type mice and human apoM-containing HDL, respectively. The binding, uptake, and transport of 125I-HDL by human aortic endothelial cells was increased by an S1P1 agonist but decreased by an S1P1 inhibitor. Silencing of SR-BI (scavenger receptor BI) abrogated the stimulation of 125I-HDL transport by the S1P1 agonist. Compared with control mice, that is, apoE-haploinsufficient mice without endothelium-specific knockin of S1P1, apoE-haploinsufficient mice with endothelium-specific knockin of S1P1 showed decreased transport of Evans Blue but increased transport of HDL from blood into the peritoneal cavity and SR-BI expression in the aortal endothelium.Conclusions:ApoM and S1P1 promote transendothelial HDL transport. Their opposite effect on transendothelial transport of albumin and HDL indicates that HDL passes endothelial barriers by specific mechanisms rather than passive filtration.

U2 - 10.1161/ATVBAHA.121.316725

DO - 10.1161/ATVBAHA.121.316725

M3 - SCORING: Journal article

C2 - 34407633

VL - 41

SP - e468-e479

JO - ARTERIOSCL THROM VAS

JF - ARTERIOSCL THROM VAS

SN - 1079-5642

IS - 10

ER -