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Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes

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Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes. / Paulsen, Friedrich; Pufe, Thomas; Conradi, Lenard; Varoga, Deike; Tsokos, Michael; Papendieck, Jann; Petersen, Wolf.

In: J PATHOL, Vol. 198, No. 3, 11.2002, p. 369-77.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Paulsen, F, Pufe, T, Conradi, L, Varoga, D, Tsokos, M, Papendieck, J & Petersen, W 2002, 'Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes', J PATHOL, vol. 198, no. 3, pp. 369-77. https://doi.org/10.1002/path.1224

APA

Paulsen, F., Pufe, T., Conradi, L., Varoga, D., Tsokos, M., Papendieck, J., & Petersen, W. (2002). Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes. J PATHOL, 198(3), 369-77. https://doi.org/10.1002/path.1224

Vancouver

Paulsen F, Pufe T, Conradi L, Varoga D, Tsokos M, Papendieck J et al. Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes. J PATHOL. 2002 Nov;198(3):369-77. https://doi.org/10.1002/path.1224

Bibtex

@article{88f8481ad5da4cba9eeb888640e04dc1,
title = "Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes",
abstract = "The objective of this study was to determine the expression and production of antimicrobial peptides by healthy and inflamed human synovial membranes. Deposition of the antimicrobial peptides lysozyme, lactoferrin, secretory phospholipase A(2) (sPA(2)), matrilysin (MMP7), human neutrophil alpha-defensins 1-3 (HNP 1-3), human beta-defensin 1 (HBD-1), and human beta-defensin 2 (HBD-2) was determined by immunohistochemistry. Expression of mRNA for the antimicrobial peptides bactericidal permeability-increasing protein (BPI), heparin binding protein (CAP37), human cationic antimicrobial protein (LL37), human alpha-defensin 5 (HD5), human alpha-defensin 6 (HD6), HBD-1, HBD-2, and human beta-defensin 3 (HBD-3) was analysed by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR revealed CAP37 and HBD-1 mRNA in samples of healthy synovial membrane. Additionally, HBD-3 and/or LL37 mRNA was detected in synovial membrane samples from patients with pyogenic arthritis (PA), osteoarthritis (OA) or rheumatoid arthritis (RA). BPI, HD5, HD6, and HBD-2 mRNAs were absent from all samples investigated. Immunohistochemistry identified lysozyme, lactoferrin, sPA(2), and MMP7 in type A synoviocytes of all samples. HBD-1 was only present in type B synoviocytes of some of the samples. Immunoreactive HBD-2 peptide was only visible in some inflamed samples. HNP1-3 was detected in both healthy and inflamed synovial membranes. The data suggest that human synovial membranes produce a broad spectrum of antimicrobial peptides. Under inflammatory conditions, the expression pattern changes, with induction of HBD-3 in PA (LL37 in RA; HBD-3 and LL37 in OA) as well as down-regulation of HBD-1. HBD-3 holds therapeutic potential in PA as it has a broad spectrum of antimicrobial activity and accelerates epithelial healing. However, caution is appropriate since defensins also promote fibrin formation and cell proliferation - key elements in joint infection. Clarification of the role of antimicrobial peptides in OA and RA will require further investigation.",
keywords = "Anti-Bacterial Agents/metabolism, Arthritis/metabolism, Arthritis, Infectious/metabolism, Arthritis, Rheumatoid/metabolism, Cell Culture Techniques, Defensins/metabolism, Humans, Immunoenzyme Techniques, Knee Joint/metabolism, Osteoarthritis, Knee/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Synovial Membrane/metabolism",
author = "Friedrich Paulsen and Thomas Pufe and Lenard Conradi and Deike Varoga and Michael Tsokos and Jann Papendieck and Wolf Petersen",
note = "Copyright 2002 John Wiley & Sons, Ltd.",
year = "2002",
month = nov,
doi = "10.1002/path.1224",
language = "English",
volume = "198",
pages = "369--77",
journal = "J PATHOL",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes

AU - Paulsen, Friedrich

AU - Pufe, Thomas

AU - Conradi, Lenard

AU - Varoga, Deike

AU - Tsokos, Michael

AU - Papendieck, Jann

AU - Petersen, Wolf

N1 - Copyright 2002 John Wiley & Sons, Ltd.

PY - 2002/11

Y1 - 2002/11

N2 - The objective of this study was to determine the expression and production of antimicrobial peptides by healthy and inflamed human synovial membranes. Deposition of the antimicrobial peptides lysozyme, lactoferrin, secretory phospholipase A(2) (sPA(2)), matrilysin (MMP7), human neutrophil alpha-defensins 1-3 (HNP 1-3), human beta-defensin 1 (HBD-1), and human beta-defensin 2 (HBD-2) was determined by immunohistochemistry. Expression of mRNA for the antimicrobial peptides bactericidal permeability-increasing protein (BPI), heparin binding protein (CAP37), human cationic antimicrobial protein (LL37), human alpha-defensin 5 (HD5), human alpha-defensin 6 (HD6), HBD-1, HBD-2, and human beta-defensin 3 (HBD-3) was analysed by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR revealed CAP37 and HBD-1 mRNA in samples of healthy synovial membrane. Additionally, HBD-3 and/or LL37 mRNA was detected in synovial membrane samples from patients with pyogenic arthritis (PA), osteoarthritis (OA) or rheumatoid arthritis (RA). BPI, HD5, HD6, and HBD-2 mRNAs were absent from all samples investigated. Immunohistochemistry identified lysozyme, lactoferrin, sPA(2), and MMP7 in type A synoviocytes of all samples. HBD-1 was only present in type B synoviocytes of some of the samples. Immunoreactive HBD-2 peptide was only visible in some inflamed samples. HNP1-3 was detected in both healthy and inflamed synovial membranes. The data suggest that human synovial membranes produce a broad spectrum of antimicrobial peptides. Under inflammatory conditions, the expression pattern changes, with induction of HBD-3 in PA (LL37 in RA; HBD-3 and LL37 in OA) as well as down-regulation of HBD-1. HBD-3 holds therapeutic potential in PA as it has a broad spectrum of antimicrobial activity and accelerates epithelial healing. However, caution is appropriate since defensins also promote fibrin formation and cell proliferation - key elements in joint infection. Clarification of the role of antimicrobial peptides in OA and RA will require further investigation.

AB - The objective of this study was to determine the expression and production of antimicrobial peptides by healthy and inflamed human synovial membranes. Deposition of the antimicrobial peptides lysozyme, lactoferrin, secretory phospholipase A(2) (sPA(2)), matrilysin (MMP7), human neutrophil alpha-defensins 1-3 (HNP 1-3), human beta-defensin 1 (HBD-1), and human beta-defensin 2 (HBD-2) was determined by immunohistochemistry. Expression of mRNA for the antimicrobial peptides bactericidal permeability-increasing protein (BPI), heparin binding protein (CAP37), human cationic antimicrobial protein (LL37), human alpha-defensin 5 (HD5), human alpha-defensin 6 (HD6), HBD-1, HBD-2, and human beta-defensin 3 (HBD-3) was analysed by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR revealed CAP37 and HBD-1 mRNA in samples of healthy synovial membrane. Additionally, HBD-3 and/or LL37 mRNA was detected in synovial membrane samples from patients with pyogenic arthritis (PA), osteoarthritis (OA) or rheumatoid arthritis (RA). BPI, HD5, HD6, and HBD-2 mRNAs were absent from all samples investigated. Immunohistochemistry identified lysozyme, lactoferrin, sPA(2), and MMP7 in type A synoviocytes of all samples. HBD-1 was only present in type B synoviocytes of some of the samples. Immunoreactive HBD-2 peptide was only visible in some inflamed samples. HNP1-3 was detected in both healthy and inflamed synovial membranes. The data suggest that human synovial membranes produce a broad spectrum of antimicrobial peptides. Under inflammatory conditions, the expression pattern changes, with induction of HBD-3 in PA (LL37 in RA; HBD-3 and LL37 in OA) as well as down-regulation of HBD-1. HBD-3 holds therapeutic potential in PA as it has a broad spectrum of antimicrobial activity and accelerates epithelial healing. However, caution is appropriate since defensins also promote fibrin formation and cell proliferation - key elements in joint infection. Clarification of the role of antimicrobial peptides in OA and RA will require further investigation.

KW - Anti-Bacterial Agents/metabolism

KW - Arthritis/metabolism

KW - Arthritis, Infectious/metabolism

KW - Arthritis, Rheumatoid/metabolism

KW - Cell Culture Techniques

KW - Defensins/metabolism

KW - Humans

KW - Immunoenzyme Techniques

KW - Knee Joint/metabolism

KW - Osteoarthritis, Knee/metabolism

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Synovial Membrane/metabolism

U2 - 10.1002/path.1224

DO - 10.1002/path.1224

M3 - SCORING: Journal article

C2 - 12375270

VL - 198

SP - 369

EP - 377

JO - J PATHOL

JF - J PATHOL

SN - 0022-3417

IS - 3

ER -