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Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis

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Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis. / Lopens, Steffi; Schierack, Peter; Krause, Jenny; Piaszczyński, Michał; Król, Robert; Staroń, Robert; Krupa, Łukasz; Gutkowski, Krzysztof; Kruk, Beata; Grąt, Michał; Krawczyk, Marek; Patkowski, Waldemar; Glaser, Fabian; Rödiger, Stefan; Grossmann, Kai; Pająk, Jacek; Milkiewicz, Piotr; Lammert, Frank; Zieniewicz, Krzysztof; Schramm, Christoph; Roggenbuck, Dirk; Krawczyk, Marcin.

In: CLIN CHIM ACTA, Vol. 562, 15.08.2024, p. 119841.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lopens, S, Schierack, P, Krause, J, Piaszczyński, M, Król, R, Staroń, R, Krupa, Ł, Gutkowski, K, Kruk, B, Grąt, M, Krawczyk, M, Patkowski, W, Glaser, F, Rödiger, S, Grossmann, K, Pająk, J, Milkiewicz, P, Lammert, F, Zieniewicz, K, Schramm, C, Roggenbuck, D & Krawczyk, M 2024, 'Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis', CLIN CHIM ACTA, vol. 562, pp. 119841. https://doi.org/10.1016/j.cca.2024.119841

APA

Lopens, S., Schierack, P., Krause, J., Piaszczyński, M., Król, R., Staroń, R., Krupa, Ł., Gutkowski, K., Kruk, B., Grąt, M., Krawczyk, M., Patkowski, W., Glaser, F., Rödiger, S., Grossmann, K., Pająk, J., Milkiewicz, P., Lammert, F., Zieniewicz, K., ... Krawczyk, M. (2024). Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis. CLIN CHIM ACTA, 562, 119841. https://doi.org/10.1016/j.cca.2024.119841

Vancouver

Lopens S, Schierack P, Krause J, Piaszczyński M, Król R, Staroń R et al. Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis. CLIN CHIM ACTA. 2024 Aug 15;562:119841. https://doi.org/10.1016/j.cca.2024.119841

Bibtex

@article{de05c6ab16884504b7f157a420a39168,
title = "Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis",
abstract = "BACKGROUND: Glycoprotein-2 (GP2) IgA is a predictor of disease severity in primary sclerosing cholangitis (PSC). We examined GP2's occurrence in the biliary tract, the site of inflammation.METHODS: GP2 was analyzed using ELISA, immunoblotting, mass spectrometry, and immunohistochemistry. The samples included: 20 bile and 30 serum samples from PSC patients, 23 bile and 11 serum samples from patients with gallstone disease (GD), 15 bile samples from healthy individuals undergoing liver-donation surgery (HILD), 20 extracts of gallstones (GE) obtained during cholecystectomy, and 101 blood-donor sera.RESULTS: Biliary GP2 concentrations were significantly higher in patients with PSC and GD than in HILD (p < 0.0001). Serum GP2 levels were similar in PSC and GD patients, and controls, but lower than in bile (p < 0.0001). GP2 was detected in all 20 GEs. Mass spectrometry identified GP2 in the bile of 2 randomly selected GD and 2 PSC patients, and in none of 2 HILD samples. GP2 was found in peribiliary glands in 8 out of 12 PSC patients, showing morphological changes in acinar cells, but not in GD-gallbladders.CONCLUSIONS: GP2 is present in bile of PSC and GD patients. It is synthesized in the peribiliary glands of PSC patients, supporting a pathogenic role for biliary GP2 in PSC.",
author = "Steffi Lopens and Peter Schierack and Jenny Krause and Micha{\l} Piaszczy{\'n}ski and Robert Kr{\'o}l and Robert Staro{\'n} and {\L}ukasz Krupa and Krzysztof Gutkowski and Beata Kruk and Micha{\l} Gr{\c a}t and Marek Krawczyk and Waldemar Patkowski and Fabian Glaser and Stefan R{\"o}diger and Kai Grossmann and Jacek Paj{\c a}k and Piotr Milkiewicz and Frank Lammert and Krzysztof Zieniewicz and Christoph Schramm and Dirk Roggenbuck and Marcin Krawczyk",
note = "Copyright {\textcopyright} 2024 The Author(s). Published by Elsevier B.V. All rights reserved.",
year = "2024",
month = aug,
day = "15",
doi = "10.1016/j.cca.2024.119841",
language = "English",
volume = "562",
pages = "119841",
journal = "CLIN CHIM ACTA",
issn = "0009-8981",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis

AU - Lopens, Steffi

AU - Schierack, Peter

AU - Krause, Jenny

AU - Piaszczyński, Michał

AU - Król, Robert

AU - Staroń, Robert

AU - Krupa, Łukasz

AU - Gutkowski, Krzysztof

AU - Kruk, Beata

AU - Grąt, Michał

AU - Krawczyk, Marek

AU - Patkowski, Waldemar

AU - Glaser, Fabian

AU - Rödiger, Stefan

AU - Grossmann, Kai

AU - Pająk, Jacek

AU - Milkiewicz, Piotr

AU - Lammert, Frank

AU - Zieniewicz, Krzysztof

AU - Schramm, Christoph

AU - Roggenbuck, Dirk

AU - Krawczyk, Marcin

N1 - Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.

PY - 2024/8/15

Y1 - 2024/8/15

N2 - BACKGROUND: Glycoprotein-2 (GP2) IgA is a predictor of disease severity in primary sclerosing cholangitis (PSC). We examined GP2's occurrence in the biliary tract, the site of inflammation.METHODS: GP2 was analyzed using ELISA, immunoblotting, mass spectrometry, and immunohistochemistry. The samples included: 20 bile and 30 serum samples from PSC patients, 23 bile and 11 serum samples from patients with gallstone disease (GD), 15 bile samples from healthy individuals undergoing liver-donation surgery (HILD), 20 extracts of gallstones (GE) obtained during cholecystectomy, and 101 blood-donor sera.RESULTS: Biliary GP2 concentrations were significantly higher in patients with PSC and GD than in HILD (p < 0.0001). Serum GP2 levels were similar in PSC and GD patients, and controls, but lower than in bile (p < 0.0001). GP2 was detected in all 20 GEs. Mass spectrometry identified GP2 in the bile of 2 randomly selected GD and 2 PSC patients, and in none of 2 HILD samples. GP2 was found in peribiliary glands in 8 out of 12 PSC patients, showing morphological changes in acinar cells, but not in GD-gallbladders.CONCLUSIONS: GP2 is present in bile of PSC and GD patients. It is synthesized in the peribiliary glands of PSC patients, supporting a pathogenic role for biliary GP2 in PSC.

AB - BACKGROUND: Glycoprotein-2 (GP2) IgA is a predictor of disease severity in primary sclerosing cholangitis (PSC). We examined GP2's occurrence in the biliary tract, the site of inflammation.METHODS: GP2 was analyzed using ELISA, immunoblotting, mass spectrometry, and immunohistochemistry. The samples included: 20 bile and 30 serum samples from PSC patients, 23 bile and 11 serum samples from patients with gallstone disease (GD), 15 bile samples from healthy individuals undergoing liver-donation surgery (HILD), 20 extracts of gallstones (GE) obtained during cholecystectomy, and 101 blood-donor sera.RESULTS: Biliary GP2 concentrations were significantly higher in patients with PSC and GD than in HILD (p < 0.0001). Serum GP2 levels were similar in PSC and GD patients, and controls, but lower than in bile (p < 0.0001). GP2 was detected in all 20 GEs. Mass spectrometry identified GP2 in the bile of 2 randomly selected GD and 2 PSC patients, and in none of 2 HILD samples. GP2 was found in peribiliary glands in 8 out of 12 PSC patients, showing morphological changes in acinar cells, but not in GD-gallbladders.CONCLUSIONS: GP2 is present in bile of PSC and GD patients. It is synthesized in the peribiliary glands of PSC patients, supporting a pathogenic role for biliary GP2 in PSC.

U2 - 10.1016/j.cca.2024.119841

DO - 10.1016/j.cca.2024.119841

M3 - SCORING: Journal article

C2 - 38964568

VL - 562

SP - 119841

JO - CLIN CHIM ACTA

JF - CLIN CHIM ACTA

SN - 0009-8981

ER -