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Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany

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Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany. / Xanthopoulou, Kyriaki; Carattoli, Alessandra; Wille, Julia; Biehl, Lena M; Rohde, Holger; Farowski, Fedja; Krut, Oleg; Villa, Laura; Feudi, Claudia; Seifert, Harald; Higgins, Paul G.

In: ANTIBIOTICS-BASEL, Vol. 9, No. 10, 05.10.2020.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Xanthopoulou, K, Carattoli, A, Wille, J, Biehl, LM, Rohde, H, Farowski, F, Krut, O, Villa, L, Feudi, C, Seifert, H & Higgins, PG 2020, 'Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany', ANTIBIOTICS-BASEL, vol. 9, no. 10. https://doi.org/10.3390/antibiotics9100675

APA

Xanthopoulou, K., Carattoli, A., Wille, J., Biehl, L. M., Rohde, H., Farowski, F., Krut, O., Villa, L., Feudi, C., Seifert, H., & Higgins, P. G. (2020). Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany. ANTIBIOTICS-BASEL, 9(10). https://doi.org/10.3390/antibiotics9100675

Vancouver

Xanthopoulou K, Carattoli A, Wille J, Biehl LM, Rohde H, Farowski F et al. Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany. ANTIBIOTICS-BASEL. 2020 Oct 5;9(10). https://doi.org/10.3390/antibiotics9100675

Bibtex

@article{47dd1382d3594781b0b7e988e6b607bd,
title = "Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany",
abstract = "Mobile genetic elements (MGEs), especially multidrug-resistance plasmids, are major vehicles for the dissemination of antimicrobial resistance determinants. Herein, we analyse the MGEs in three extensively drug-resistant (XDR) Klebsiella pneumoniae isolates from Germany. Whole genome sequencing (WGS) is performed using Illumina and MinION platforms followed by core-genome multi-locus sequence typing (MLST). The plasmid content is analysed by conjugation, S1-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot experiments. The K. pneumoniae isolates belong to the international high-risk clone ST147 and form a cluster of closely related isolates. They harbour the blaOXA-181 carbapenemase on a ColKP3 plasmid, and 12 antibiotic resistance determinants on an multidrug-resistant (MDR) IncR plasmid with a recombinogenic nature and encoding a large number of insertion elements. The IncR plasmids within the three isolates share a high degree of homology, but present also genetic variations, such as inversion or deletion of genetic regions in close proximity to MGEs. In addition, six plasmids not harbouring any antibiotic resistance determinants are present in each isolate. Our study indicates that genetic variations can be observed within a cluster of closely related isolates, due to the dynamic nature of MGEs. The mobilome of the K. pneumoniae isolates combined with the emergence of the XDR ST147 high-risk clone have the potential to become a major challenge for global healthcare.",
author = "Kyriaki Xanthopoulou and Alessandra Carattoli and Julia Wille and Biehl, {Lena M} and Holger Rohde and Fedja Farowski and Oleg Krut and Laura Villa and Claudia Feudi and Harald Seifert and Higgins, {Paul G}",
year = "2020",
month = oct,
day = "5",
doi = "10.3390/antibiotics9100675",
language = "English",
volume = "9",
journal = "ANTIBIOTICS-BASEL",
issn = "2079-6382",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
number = "10",

}

RIS

TY - JOUR

T1 - Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany

AU - Xanthopoulou, Kyriaki

AU - Carattoli, Alessandra

AU - Wille, Julia

AU - Biehl, Lena M

AU - Rohde, Holger

AU - Farowski, Fedja

AU - Krut, Oleg

AU - Villa, Laura

AU - Feudi, Claudia

AU - Seifert, Harald

AU - Higgins, Paul G

PY - 2020/10/5

Y1 - 2020/10/5

N2 - Mobile genetic elements (MGEs), especially multidrug-resistance plasmids, are major vehicles for the dissemination of antimicrobial resistance determinants. Herein, we analyse the MGEs in three extensively drug-resistant (XDR) Klebsiella pneumoniae isolates from Germany. Whole genome sequencing (WGS) is performed using Illumina and MinION platforms followed by core-genome multi-locus sequence typing (MLST). The plasmid content is analysed by conjugation, S1-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot experiments. The K. pneumoniae isolates belong to the international high-risk clone ST147 and form a cluster of closely related isolates. They harbour the blaOXA-181 carbapenemase on a ColKP3 plasmid, and 12 antibiotic resistance determinants on an multidrug-resistant (MDR) IncR plasmid with a recombinogenic nature and encoding a large number of insertion elements. The IncR plasmids within the three isolates share a high degree of homology, but present also genetic variations, such as inversion or deletion of genetic regions in close proximity to MGEs. In addition, six plasmids not harbouring any antibiotic resistance determinants are present in each isolate. Our study indicates that genetic variations can be observed within a cluster of closely related isolates, due to the dynamic nature of MGEs. The mobilome of the K. pneumoniae isolates combined with the emergence of the XDR ST147 high-risk clone have the potential to become a major challenge for global healthcare.

AB - Mobile genetic elements (MGEs), especially multidrug-resistance plasmids, are major vehicles for the dissemination of antimicrobial resistance determinants. Herein, we analyse the MGEs in three extensively drug-resistant (XDR) Klebsiella pneumoniae isolates from Germany. Whole genome sequencing (WGS) is performed using Illumina and MinION platforms followed by core-genome multi-locus sequence typing (MLST). The plasmid content is analysed by conjugation, S1-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot experiments. The K. pneumoniae isolates belong to the international high-risk clone ST147 and form a cluster of closely related isolates. They harbour the blaOXA-181 carbapenemase on a ColKP3 plasmid, and 12 antibiotic resistance determinants on an multidrug-resistant (MDR) IncR plasmid with a recombinogenic nature and encoding a large number of insertion elements. The IncR plasmids within the three isolates share a high degree of homology, but present also genetic variations, such as inversion or deletion of genetic regions in close proximity to MGEs. In addition, six plasmids not harbouring any antibiotic resistance determinants are present in each isolate. Our study indicates that genetic variations can be observed within a cluster of closely related isolates, due to the dynamic nature of MGEs. The mobilome of the K. pneumoniae isolates combined with the emergence of the XDR ST147 high-risk clone have the potential to become a major challenge for global healthcare.

U2 - 10.3390/antibiotics9100675

DO - 10.3390/antibiotics9100675

M3 - SCORING: Journal article

C2 - 33028048

VL - 9

JO - ANTIBIOTICS-BASEL

JF - ANTIBIOTICS-BASEL

SN - 2079-6382

IS - 10

ER -