Research ExplorerPublicationsAnimal serum-free culture conditions for isolation and expan...

Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM

Standard

Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. / Müller, I; Kordowich, S; Holzwarth, C; Spano, C; Isensee, G; Staiber, A; Viebahn, S; Gieseke, F; Langer, H; Gawaz, M P; Horwitz, E M; Conte, P; Handgretinger, R; Dominici, M.

In: CYTOTHERAPY, Vol. 8, No. 5, 01.01.2006, p. 437-44.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Transfer › peer-review

Harvard

Müller, I, Kordowich, S, Holzwarth, C, Spano, C, Isensee, G, Staiber, A, Viebahn, S, Gieseke, F, Langer, H, Gawaz, MP, Horwitz, EM, Conte, P, Handgretinger, R & Dominici, M 2006, 'Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM', CYTOTHERAPY, vol. 8, no. 5, pp. 437-44. https://doi.org/10.1080/14653240600920782

APA

Müller, I., Kordowich, S., Holzwarth, C., Spano, C., Isensee, G., Staiber, A., Viebahn, S., Gieseke, F., Langer, H., Gawaz, M. P., Horwitz, E. M., Conte, P., Handgretinger, R., & Dominici, M. (2006). Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. CYTOTHERAPY, 8(5), 437-44. https://doi.org/10.1080/14653240600920782

Vancouver

Müller I, Kordowich S, Holzwarth C, Spano C, Isensee G, Staiber A et al. Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. CYTOTHERAPY. 2006 Jan 1;8(5):437-44. https://doi.org/10.1080/14653240600920782

Bibtex

@article{92e5f5140a864923afaf3db07680fcef,
title = "Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM",
abstract = "BACKGROUND: Multipotent mesenchymal stromal cells (MSC) have become important tools in regenerative and transplantation medicine. Rapidly increasing numbers of patients are receiving in vitro-expanded MSC. Culture conditions typically include FSC because human serum does not fully support growth of human MSC in vitro (MSC(FCS)). Concerns regarding BSE, other infectious complications and host immune reactions have fueled investigation of alternative culture supplements.METHODS: As PDGF has long been identified as a growth factor for MSC, we tested media supplementation with platelet lysate for support of MSC proliferation.RESULTS: We found that primary cultures of BM-derived MSC can be established with animal serum-free media containing fresh frozen plasma and platelets (MSC(FFPP)). Moreover, MSC(FFPP) showed vigorous proliferation that was superior to classical culture conditions containing FCS. MSC(FFPP) morphology was equivalent to MSC(FCS), and MSC(FFPP) expressed CD73, CD90, CD105, CD106, CD146 and HLA-ABC while being negative for CD34, CD45 and surface HLA-DR, as expected. In addition to being phenotypically identical, MSC(FFPP) could efficiently differentiate into adipocytes and osteoblasts. In terms of immune regulatory properties, MSC(FFPP) were indistinguishable from MSC(FCS). Proliferation of PBMC induced by IL-2 in combination with OKT-3 or by PHA was inhibited in the presence of MSC(FFPP).DISCUSSION: Taken together, FCS can be replaced safely by FFPP in cultures of MSC for clinical purposes.",
keywords = "Antigens, Differentiation, Bone Marrow Cells, Cell Differentiation, Cell Proliferation, Cell Separation, Cells, Cultured, Culture Media, Serum-Free, Humans, Mesoderm, Multipotent Stem Cells, Platelet-Derived Growth Factor, Stromal Cells",
author = "I M{\"u}ller and S Kordowich and C Holzwarth and C Spano and G Isensee and A Staiber and S Viebahn and F Gieseke and H Langer and Gawaz, {M P} and Horwitz, {E M} and P Conte and R Handgretinger and M Dominici",
year = "2006",
month = jan,
day = "1",
doi = "10.1080/14653240600920782",
language = "English",
volume = "8",
pages = "437--44",
journal = "CYTOTHERAPY",
issn = "1465-3249",
publisher = "informa healthcare",
number = "5",

}

RIS

TY - JOUR

T1 - Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM

AU - Müller, I

AU - Kordowich, S

AU - Holzwarth, C

AU - Spano, C

AU - Isensee, G

AU - Staiber, A

AU - Viebahn, S

AU - Gieseke, F

AU - Langer, H

AU - Gawaz, M P

AU - Horwitz, E M

AU - Conte, P

AU - Handgretinger, R

AU - Dominici, M

PY - 2006/1/1

Y1 - 2006/1/1

N2 - BACKGROUND: Multipotent mesenchymal stromal cells (MSC) have become important tools in regenerative and transplantation medicine. Rapidly increasing numbers of patients are receiving in vitro-expanded MSC. Culture conditions typically include FSC because human serum does not fully support growth of human MSC in vitro (MSC(FCS)). Concerns regarding BSE, other infectious complications and host immune reactions have fueled investigation of alternative culture supplements.METHODS: As PDGF has long been identified as a growth factor for MSC, we tested media supplementation with platelet lysate for support of MSC proliferation.RESULTS: We found that primary cultures of BM-derived MSC can be established with animal serum-free media containing fresh frozen plasma and platelets (MSC(FFPP)). Moreover, MSC(FFPP) showed vigorous proliferation that was superior to classical culture conditions containing FCS. MSC(FFPP) morphology was equivalent to MSC(FCS), and MSC(FFPP) expressed CD73, CD90, CD105, CD106, CD146 and HLA-ABC while being negative for CD34, CD45 and surface HLA-DR, as expected. In addition to being phenotypically identical, MSC(FFPP) could efficiently differentiate into adipocytes and osteoblasts. In terms of immune regulatory properties, MSC(FFPP) were indistinguishable from MSC(FCS). Proliferation of PBMC induced by IL-2 in combination with OKT-3 or by PHA was inhibited in the presence of MSC(FFPP).DISCUSSION: Taken together, FCS can be replaced safely by FFPP in cultures of MSC for clinical purposes.

AB - BACKGROUND: Multipotent mesenchymal stromal cells (MSC) have become important tools in regenerative and transplantation medicine. Rapidly increasing numbers of patients are receiving in vitro-expanded MSC. Culture conditions typically include FSC because human serum does not fully support growth of human MSC in vitro (MSC(FCS)). Concerns regarding BSE, other infectious complications and host immune reactions have fueled investigation of alternative culture supplements.METHODS: As PDGF has long been identified as a growth factor for MSC, we tested media supplementation with platelet lysate for support of MSC proliferation.RESULTS: We found that primary cultures of BM-derived MSC can be established with animal serum-free media containing fresh frozen plasma and platelets (MSC(FFPP)). Moreover, MSC(FFPP) showed vigorous proliferation that was superior to classical culture conditions containing FCS. MSC(FFPP) morphology was equivalent to MSC(FCS), and MSC(FFPP) expressed CD73, CD90, CD105, CD106, CD146 and HLA-ABC while being negative for CD34, CD45 and surface HLA-DR, as expected. In addition to being phenotypically identical, MSC(FFPP) could efficiently differentiate into adipocytes and osteoblasts. In terms of immune regulatory properties, MSC(FFPP) were indistinguishable from MSC(FCS). Proliferation of PBMC induced by IL-2 in combination with OKT-3 or by PHA was inhibited in the presence of MSC(FFPP).DISCUSSION: Taken together, FCS can be replaced safely by FFPP in cultures of MSC for clinical purposes.

KW - Antigens, Differentiation

KW - Bone Marrow Cells

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cell Separation

KW - Cells, Cultured

KW - Culture Media, Serum-Free

KW - Humans

KW - Mesoderm

KW - Multipotent Stem Cells

KW - Platelet-Derived Growth Factor

KW - Stromal Cells

U2 - 10.1080/14653240600920782

DO - 10.1080/14653240600920782

M3 - SCORING: Journal article

C2 - 17050248

VL - 8

SP - 437

EP - 444

JO - CYTOTHERAPY

JF - CYTOTHERAPY

SN - 1465-3249

IS - 5

ER -