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Angiogenic activation of valvular endothelial cells in aortic valve stenosis

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Angiogenic activation of valvular endothelial cells in aortic valve stenosis. / Chalajour, Fariba; Treede, Hendrik; Ebrahimnejad, Alireza; Lauke, Heidrun; Reichenspurner, Hermann; Ergun, Suleyman.

In: EXP CELL RES, Vol. 298, No. 2, 15.08.2004, p. 455-64.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Chalajour, F, Treede, H, Ebrahimnejad, A, Lauke, H, Reichenspurner, H & Ergun, S 2004, 'Angiogenic activation of valvular endothelial cells in aortic valve stenosis', EXP CELL RES, vol. 298, no. 2, pp. 455-64. https://doi.org/10.1016/j.yexcr.2004.04.034

APA

Chalajour, F., Treede, H., Ebrahimnejad, A., Lauke, H., Reichenspurner, H., & Ergun, S. (2004). Angiogenic activation of valvular endothelial cells in aortic valve stenosis. EXP CELL RES, 298(2), 455-64. https://doi.org/10.1016/j.yexcr.2004.04.034

Vancouver

Chalajour F, Treede H, Ebrahimnejad A, Lauke H, Reichenspurner H, Ergun S. Angiogenic activation of valvular endothelial cells in aortic valve stenosis. EXP CELL RES. 2004 Aug 15;298(2):455-64. https://doi.org/10.1016/j.yexcr.2004.04.034

Bibtex

@article{a5978fb232ee48a59f1c4e66e9aa1721,
title = "Angiogenic activation of valvular endothelial cells in aortic valve stenosis",
abstract = "Here, we demonstrate the angiogenic response of valvular endothelial cells to aortic valve (AV) stenosis using a new ex vivo model of aortic leaflets. Histological analysis revealed neovascularization within the cusps of stenotic but not of non-stenotic aortic valves. Correspondingly, the number of capillary-like outgrowth in 3D collagen gel was significantly higher in stenotic than in non-stenotic valves. Capillary-like sprouting was developed significantly faster in stenotic than in non-stenotic valves. New capillary sprouts from stenotic aortic valves exhibited the endothelial cell markers CD31, CD34 and von-Willebrand factor (vWF) as well as carcinoembryonic antigen cell adhesion molecule-1 (CEACAM1), Tie-2 and angiogenesis inhibitor endostatin. Western blot analyses revealed a significant increase of CEACAM1 and endostatin in stenotic aortic valve tissue. Electron microscopic examinations demonstrate that these capillary-like tubes are formed by endothelial cells containing Weibel-Palade bodies. Remarkably, inter-endothelial junctions are established and basement membrane material is partially deposited on the basal side of the endothelial tubes. Our data demonstrate the capillary-like sprout formation from aortic valves and suggest a role of angiogenesis in the pathogenesis of aortic valve stenosis. These data provide new insights into the mechanisms of valvular disorders and open new perspectives for prevention and early treatment of calcified aortic stenosis.",
keywords = "Aged, Antigens, CD/metabolism, Antigens, CD34/metabolism, Antigens, Differentiation/metabolism, Aortic Valve/growth & development, Aortic Valve Stenosis/metabolism, Basement Membrane/metabolism, Capillaries/metabolism, Cell Adhesion Molecules, Endostatins/metabolism, Endothelium, Vascular/metabolism, Female, Humans, Intercellular Junctions/metabolism, Male, Microscopy, Electron, Models, Biological, Neovascularization, Pathologic/metabolism, Organ Culture Techniques, Platelet Endothelial Cell Adhesion Molecule-1/metabolism, Receptor, TIE-2/metabolism, Weibel-Palade Bodies/metabolism, von Willebrand Factor/metabolism",
author = "Fariba Chalajour and Hendrik Treede and Alireza Ebrahimnejad and Heidrun Lauke and Hermann Reichenspurner and Suleyman Ergun",
year = "2004",
month = aug,
day = "15",
doi = "10.1016/j.yexcr.2004.04.034",
language = "English",
volume = "298",
pages = "455--64",
journal = "EXP CELL RES",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Angiogenic activation of valvular endothelial cells in aortic valve stenosis

AU - Chalajour, Fariba

AU - Treede, Hendrik

AU - Ebrahimnejad, Alireza

AU - Lauke, Heidrun

AU - Reichenspurner, Hermann

AU - Ergun, Suleyman

PY - 2004/8/15

Y1 - 2004/8/15

N2 - Here, we demonstrate the angiogenic response of valvular endothelial cells to aortic valve (AV) stenosis using a new ex vivo model of aortic leaflets. Histological analysis revealed neovascularization within the cusps of stenotic but not of non-stenotic aortic valves. Correspondingly, the number of capillary-like outgrowth in 3D collagen gel was significantly higher in stenotic than in non-stenotic valves. Capillary-like sprouting was developed significantly faster in stenotic than in non-stenotic valves. New capillary sprouts from stenotic aortic valves exhibited the endothelial cell markers CD31, CD34 and von-Willebrand factor (vWF) as well as carcinoembryonic antigen cell adhesion molecule-1 (CEACAM1), Tie-2 and angiogenesis inhibitor endostatin. Western blot analyses revealed a significant increase of CEACAM1 and endostatin in stenotic aortic valve tissue. Electron microscopic examinations demonstrate that these capillary-like tubes are formed by endothelial cells containing Weibel-Palade bodies. Remarkably, inter-endothelial junctions are established and basement membrane material is partially deposited on the basal side of the endothelial tubes. Our data demonstrate the capillary-like sprout formation from aortic valves and suggest a role of angiogenesis in the pathogenesis of aortic valve stenosis. These data provide new insights into the mechanisms of valvular disorders and open new perspectives for prevention and early treatment of calcified aortic stenosis.

AB - Here, we demonstrate the angiogenic response of valvular endothelial cells to aortic valve (AV) stenosis using a new ex vivo model of aortic leaflets. Histological analysis revealed neovascularization within the cusps of stenotic but not of non-stenotic aortic valves. Correspondingly, the number of capillary-like outgrowth in 3D collagen gel was significantly higher in stenotic than in non-stenotic valves. Capillary-like sprouting was developed significantly faster in stenotic than in non-stenotic valves. New capillary sprouts from stenotic aortic valves exhibited the endothelial cell markers CD31, CD34 and von-Willebrand factor (vWF) as well as carcinoembryonic antigen cell adhesion molecule-1 (CEACAM1), Tie-2 and angiogenesis inhibitor endostatin. Western blot analyses revealed a significant increase of CEACAM1 and endostatin in stenotic aortic valve tissue. Electron microscopic examinations demonstrate that these capillary-like tubes are formed by endothelial cells containing Weibel-Palade bodies. Remarkably, inter-endothelial junctions are established and basement membrane material is partially deposited on the basal side of the endothelial tubes. Our data demonstrate the capillary-like sprout formation from aortic valves and suggest a role of angiogenesis in the pathogenesis of aortic valve stenosis. These data provide new insights into the mechanisms of valvular disorders and open new perspectives for prevention and early treatment of calcified aortic stenosis.

KW - Aged

KW - Antigens, CD/metabolism

KW - Antigens, CD34/metabolism

KW - Antigens, Differentiation/metabolism

KW - Aortic Valve/growth & development

KW - Aortic Valve Stenosis/metabolism

KW - Basement Membrane/metabolism

KW - Capillaries/metabolism

KW - Cell Adhesion Molecules

KW - Endostatins/metabolism

KW - Endothelium, Vascular/metabolism

KW - Female

KW - Humans

KW - Intercellular Junctions/metabolism

KW - Male

KW - Microscopy, Electron

KW - Models, Biological

KW - Neovascularization, Pathologic/metabolism

KW - Organ Culture Techniques

KW - Platelet Endothelial Cell Adhesion Molecule-1/metabolism

KW - Receptor, TIE-2/metabolism

KW - Weibel-Palade Bodies/metabolism

KW - von Willebrand Factor/metabolism

U2 - 10.1016/j.yexcr.2004.04.034

DO - 10.1016/j.yexcr.2004.04.034

M3 - SCORING: Journal article

C2 - 15265693

VL - 298

SP - 455

EP - 464

JO - EXP CELL RES

JF - EXP CELL RES

SN - 0014-4827

IS - 2

ER -