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Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

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Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19. / Meinberger, Denise; Koch, Manuel; Roth, Annika; Hermes, Gabriele; Stemler, Jannik; Cornely, Oliver A; Streichert, Thomas; Klatt, Andreas R.

In: SCI REP-UK, Vol. 11, No. 1, 26.04.2021, p. 8920.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Meinberger, D, Koch, M, Roth, A, Hermes, G, Stemler, J, Cornely, OA, Streichert, T & Klatt, AR 2021, 'Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19', SCI REP-UK, vol. 11, no. 1, pp. 8920. https://doi.org/10.1038/s41598-021-88356-8

APA

Meinberger, D., Koch, M., Roth, A., Hermes, G., Stemler, J., Cornely, O. A., Streichert, T., & Klatt, A. R. (2021). Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19. SCI REP-UK, 11(1), 8920. https://doi.org/10.1038/s41598-021-88356-8

Vancouver

Meinberger D, Koch M, Roth A, Hermes G, Stemler J, Cornely OA et al. Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19. SCI REP-UK. 2021 Apr 26;11(1):8920. https://doi.org/10.1038/s41598-021-88356-8

Bibtex

@article{9a80ec54e618406e84ebe4a868e2d981,
title = "Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19",
abstract = "Immunoassays are a standard diagnostic tool that assesses immunity in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection. However, immunoassays do not provide information about contaminating antigens or cross-reactions and might exhibit inaccurately high sensitivity and low specificity. We aimed to gain insight into the serological immune response of SARS-CoV-2 patients by immunoblot analysis. We analyzed serum immunoglobulins IgM, -A, and -G directed against SARS-CoV-2 proteins by immunoblot analysis from 12 infected patients. We determined IgG isotype antibodies by commercially available ELISA and assessed the clinical parameters of inflammation status and kidney and liver injury. Unexpectedly, we found no correlation between the presence of antibodies and the future course of the disease. However, attention should be paid to the parameters CRP, IL-6, and LDH. We found evidence of antibody cross-reactivity, which questions the reliability of results for serum samples that tested negative for anti-SARS-CoV-2 antibodies when assessed by immunoassays. Nevertheless, for the detection of IgG anti-SARS-CoV-2 antibodies, our data suggest that the use of the spike glycoprotein in immunoassays should be sufficient to identify positive patients. Using a combination of the spike glycoprotein and the open reading frame 8 protein could prove to be the best way of detecting anti-SARS-CoV-2 IgM antibodies.",
keywords = "Adult, Aged, Aged, 80 and over, Antibodies, Viral/immunology, Antibody Specificity, COVID-19/diagnosis, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Reproducibility of Results, SARS-CoV-2/immunology, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus/immunology, Viral Proteins/immunology",
author = "Denise Meinberger and Manuel Koch and Annika Roth and Gabriele Hermes and Jannik Stemler and Cornely, {Oliver A} and Thomas Streichert and Klatt, {Andreas R}",
year = "2021",
month = apr,
day = "26",
doi = "10.1038/s41598-021-88356-8",
language = "English",
volume = "11",
pages = "8920",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

AU - Meinberger, Denise

AU - Koch, Manuel

AU - Roth, Annika

AU - Hermes, Gabriele

AU - Stemler, Jannik

AU - Cornely, Oliver A

AU - Streichert, Thomas

AU - Klatt, Andreas R

PY - 2021/4/26

Y1 - 2021/4/26

N2 - Immunoassays are a standard diagnostic tool that assesses immunity in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection. However, immunoassays do not provide information about contaminating antigens or cross-reactions and might exhibit inaccurately high sensitivity and low specificity. We aimed to gain insight into the serological immune response of SARS-CoV-2 patients by immunoblot analysis. We analyzed serum immunoglobulins IgM, -A, and -G directed against SARS-CoV-2 proteins by immunoblot analysis from 12 infected patients. We determined IgG isotype antibodies by commercially available ELISA and assessed the clinical parameters of inflammation status and kidney and liver injury. Unexpectedly, we found no correlation between the presence of antibodies and the future course of the disease. However, attention should be paid to the parameters CRP, IL-6, and LDH. We found evidence of antibody cross-reactivity, which questions the reliability of results for serum samples that tested negative for anti-SARS-CoV-2 antibodies when assessed by immunoassays. Nevertheless, for the detection of IgG anti-SARS-CoV-2 antibodies, our data suggest that the use of the spike glycoprotein in immunoassays should be sufficient to identify positive patients. Using a combination of the spike glycoprotein and the open reading frame 8 protein could prove to be the best way of detecting anti-SARS-CoV-2 IgM antibodies.

AB - Immunoassays are a standard diagnostic tool that assesses immunity in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection. However, immunoassays do not provide information about contaminating antigens or cross-reactions and might exhibit inaccurately high sensitivity and low specificity. We aimed to gain insight into the serological immune response of SARS-CoV-2 patients by immunoblot analysis. We analyzed serum immunoglobulins IgM, -A, and -G directed against SARS-CoV-2 proteins by immunoblot analysis from 12 infected patients. We determined IgG isotype antibodies by commercially available ELISA and assessed the clinical parameters of inflammation status and kidney and liver injury. Unexpectedly, we found no correlation between the presence of antibodies and the future course of the disease. However, attention should be paid to the parameters CRP, IL-6, and LDH. We found evidence of antibody cross-reactivity, which questions the reliability of results for serum samples that tested negative for anti-SARS-CoV-2 antibodies when assessed by immunoassays. Nevertheless, for the detection of IgG anti-SARS-CoV-2 antibodies, our data suggest that the use of the spike glycoprotein in immunoassays should be sufficient to identify positive patients. Using a combination of the spike glycoprotein and the open reading frame 8 protein could prove to be the best way of detecting anti-SARS-CoV-2 IgM antibodies.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Antibodies, Viral/immunology

KW - Antibody Specificity

KW - COVID-19/diagnosis

KW - Case-Control Studies

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Reproducibility of Results

KW - SARS-CoV-2/immunology

KW - Sensitivity and Specificity

KW - Spike Glycoprotein, Coronavirus/immunology

KW - Viral Proteins/immunology

U2 - 10.1038/s41598-021-88356-8

DO - 10.1038/s41598-021-88356-8

M3 - SCORING: Journal article

C2 - 33903660

VL - 11

SP - 8920

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

IS - 1

ER -