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Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development

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Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development. / Heller, S; Finn, T P; Huber, J; Nishi, R; Geissen, M; Püschel, A W; Rohrer, H.

In: DEVELOPMENT, Vol. 121, No. 8, 08.1995, p. 2681-2693.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Heller, S, Finn, TP, Huber, J, Nishi, R, Geissen, M, Püschel, AW & Rohrer, H 1995, 'Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development', DEVELOPMENT, vol. 121, no. 8, pp. 2681-2693.

APA

Heller, S., Finn, T. P., Huber, J., Nishi, R., Geissen, M., Püschel, A. W., & Rohrer, H. (1995). Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development. DEVELOPMENT, 121(8), 2681-2693.

Vancouver

Heller S, Finn TP, Huber J, Nishi R, Geissen M, Püschel AW et al. Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development. DEVELOPMENT. 1995 Aug;121(8):2681-2693.

Bibtex

@article{323a0f47dbca4770bad77fd0edd0a8a5,
title = "Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development",
abstract = "Growth promoting activity (GPA) is a chick growth factor with low homology to mammalian ciliary neurotrophic factor (CNTF) (47% sequence identity with rat CNTF) but displays similar biological effects on neuronal development. We have isolated a chick cDNA coding for GPA receptor (GPAR alpha), a GPI-anchored protein that is 70% identical to hCNTFR alpha. Functional analysis revealed that GPAR alpha mediates several biological effects of both GPA and CNTF. Soluble GPAR alpha supports GPA- and CNTF-dependent survival of human TF-1 cells. In sympathetic neurons, GPAR alpha mediates effects of both GPA and CNTF on the expression of vasoactive intestinal peptide (VIP) as shown by the inhibition of GPA- and CNTF-mediated VIP induction upon GPAR alpha antisense RNA expression. These results demonstrate that GPAR alpha is able to mediate effects of two neurokines that are only distantly related. GPAR alpha mRNA expression is largely restricted to the nervous system and was detected in all neurons that have been shown to respond to GPA or CNTF by increased survival or differentiation, i.e. ciliary, sympathetic, sensory dorsal root, motoneurons, retinal ganglion cells and amacrine cells. Interestingly, GPAR alpha mRNA was additionally found in neuronal populations and at developmental periods not known to be influenced by GPA or CNTF, suggesting novel functions for GPAR alpha and its ligands during neurogenesis and neuron differentiation.",
keywords = "Amino Acid Sequence, Animals, Cell Differentiation/physiology, Chick Embryo, Ciliary Neurotrophic Factor, DNA, Complementary/analysis, Gene Expression, Humans, Molecular Sequence Data, Nerve Growth Factors/genetics, Nerve Tissue Proteins/genetics, Nervous System/cytology, Neurons/cytology, Rats, Receptors, Nerve Growth Factor/genetics, Sequence Homology, Amino Acid",
author = "S Heller and Finn, {T P} and J Huber and R Nishi and M Geissen and P{\"u}schel, {A W} and H Rohrer",
year = "1995",
month = aug,
language = "English",
volume = "121",
pages = "2681--2693",
journal = "DEVELOPMENT",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "8",

}

RIS

TY - JOUR

T1 - Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development

AU - Heller, S

AU - Finn, T P

AU - Huber, J

AU - Nishi, R

AU - Geissen, M

AU - Püschel, A W

AU - Rohrer, H

PY - 1995/8

Y1 - 1995/8

N2 - Growth promoting activity (GPA) is a chick growth factor with low homology to mammalian ciliary neurotrophic factor (CNTF) (47% sequence identity with rat CNTF) but displays similar biological effects on neuronal development. We have isolated a chick cDNA coding for GPA receptor (GPAR alpha), a GPI-anchored protein that is 70% identical to hCNTFR alpha. Functional analysis revealed that GPAR alpha mediates several biological effects of both GPA and CNTF. Soluble GPAR alpha supports GPA- and CNTF-dependent survival of human TF-1 cells. In sympathetic neurons, GPAR alpha mediates effects of both GPA and CNTF on the expression of vasoactive intestinal peptide (VIP) as shown by the inhibition of GPA- and CNTF-mediated VIP induction upon GPAR alpha antisense RNA expression. These results demonstrate that GPAR alpha is able to mediate effects of two neurokines that are only distantly related. GPAR alpha mRNA expression is largely restricted to the nervous system and was detected in all neurons that have been shown to respond to GPA or CNTF by increased survival or differentiation, i.e. ciliary, sympathetic, sensory dorsal root, motoneurons, retinal ganglion cells and amacrine cells. Interestingly, GPAR alpha mRNA was additionally found in neuronal populations and at developmental periods not known to be influenced by GPA or CNTF, suggesting novel functions for GPAR alpha and its ligands during neurogenesis and neuron differentiation.

AB - Growth promoting activity (GPA) is a chick growth factor with low homology to mammalian ciliary neurotrophic factor (CNTF) (47% sequence identity with rat CNTF) but displays similar biological effects on neuronal development. We have isolated a chick cDNA coding for GPA receptor (GPAR alpha), a GPI-anchored protein that is 70% identical to hCNTFR alpha. Functional analysis revealed that GPAR alpha mediates several biological effects of both GPA and CNTF. Soluble GPAR alpha supports GPA- and CNTF-dependent survival of human TF-1 cells. In sympathetic neurons, GPAR alpha mediates effects of both GPA and CNTF on the expression of vasoactive intestinal peptide (VIP) as shown by the inhibition of GPA- and CNTF-mediated VIP induction upon GPAR alpha antisense RNA expression. These results demonstrate that GPAR alpha is able to mediate effects of two neurokines that are only distantly related. GPAR alpha mRNA expression is largely restricted to the nervous system and was detected in all neurons that have been shown to respond to GPA or CNTF by increased survival or differentiation, i.e. ciliary, sympathetic, sensory dorsal root, motoneurons, retinal ganglion cells and amacrine cells. Interestingly, GPAR alpha mRNA was additionally found in neuronal populations and at developmental periods not known to be influenced by GPA or CNTF, suggesting novel functions for GPAR alpha and its ligands during neurogenesis and neuron differentiation.

KW - Amino Acid Sequence

KW - Animals

KW - Cell Differentiation/physiology

KW - Chick Embryo

KW - Ciliary Neurotrophic Factor

KW - DNA, Complementary/analysis

KW - Gene Expression

KW - Humans

KW - Molecular Sequence Data

KW - Nerve Growth Factors/genetics

KW - Nerve Tissue Proteins/genetics

KW - Nervous System/cytology

KW - Neurons/cytology

KW - Rats

KW - Receptors, Nerve Growth Factor/genetics

KW - Sequence Homology, Amino Acid

M3 - SCORING: Journal article

C2 - 7671828

VL - 121

SP - 2681

EP - 2693

JO - DEVELOPMENT

JF - DEVELOPMENT

SN - 0950-1991

IS - 8

ER -