Analysis of Activity and Expression of the NLRP3, AIM2, and NLRC4 Inflammasome in Whole Blood
Related Research units
Abstract
Pro-inflammatory caspase-1 is a key player in innate immunity. Following activation in heterogenic protein complexes called the inflammasome , caspase-1 processes IL-1β and IL-18 to their mature forms and triggers pyroptosis. Here, we describe a small-volume whole blood assay facilitating the measurement of caspase-1 activity and inflammasome-related gene expression following specific stimulation of either the NLRP3, NLRC4, or AIM2 inflammasome .
Bibliographical data
| Original language | English |
|---|---|
| Title of host publication | The Inflammasome : Methods and Protocols |
| Editors | Ali A. Abdul-Sater |
| REQUIRED books only: Number of pages | 12 |
| Place of Publication | New York, NY |
| Publisher | Humana Press |
| Publication date | 2022 |
| Edition | 1 |
| Pages | 105-116 |
| ISBN (Print) | 978-1-0716-2143-1 |
| ISBN (Electronic) | 978-1-0716-2144-8 |
| DOIs | |
| Publication status | Published - 2022 |
Comment Deanary
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
| PubMed | 35212959 |
|---|
