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An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?

Standard

An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis? / Kaberdina, Anna Chao; Szaflarski, Witold; Nierhaus, Knud H; Moll, Ingrid.

In: MOL CELL, Vol. 33, No. 2, 2, 2009, p. 227-236.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kaberdina, AC, Szaflarski, W, Nierhaus, KH & Moll, I 2009, 'An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?', MOL CELL, vol. 33, no. 2, 2, pp. 227-236. <http://www.ncbi.nlm.nih.gov/pubmed/19187763?dopt=Citation>

APA

Kaberdina, A. C., Szaflarski, W., Nierhaus, K. H., & Moll, I. (2009). An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis? MOL CELL, 33(2), 227-236. [2]. http://www.ncbi.nlm.nih.gov/pubmed/19187763?dopt=Citation

Vancouver

Kaberdina AC, Szaflarski W, Nierhaus KH, Moll I. An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis? MOL CELL. 2009;33(2):227-236. 2.

Bibtex

@article{a37a03cb8cb44def822c69a7718a00f5,
title = "An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?",
abstract = "Translation of leaderless mRNAs, lacking ribosomal recruitment signals other than the 5'-terminal AUG-initiating codon, occurs in all three domains of life. Contemporary leaderless mRNAs may therefore be viewed as molecular fossils resembling ancestral mRNAs. Here, we analyzed the phenomenon of sustained translation of a leaderless mRNA in the presence of the antibiotic kasugamycin. Unexpected from the known in vitro effects of the drug, kasugamycin induced the formation of stable approximately 61S ribosomes in vivo, which were proficient in selectively translating leaderless mRNA. 61S particles are devoid of more than six proteins of the small subunit, including the functionally important proteins S1 and S12. The lack of these proteins could be reconciled with structural changes in the 16S rRNA. These studies provide in vivo evidence for the functionality of ribosomes devoid of multiple proteins and shed light on the evolutionary history of ribosomes.",
author = "Kaberdina, {Anna Chao} and Witold Szaflarski and Nierhaus, {Knud H} and Ingrid Moll",
year = "2009",
language = "Deutsch",
volume = "33",
pages = "227--236",
journal = "MOL CELL",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?

AU - Kaberdina, Anna Chao

AU - Szaflarski, Witold

AU - Nierhaus, Knud H

AU - Moll, Ingrid

PY - 2009

Y1 - 2009

N2 - Translation of leaderless mRNAs, lacking ribosomal recruitment signals other than the 5'-terminal AUG-initiating codon, occurs in all three domains of life. Contemporary leaderless mRNAs may therefore be viewed as molecular fossils resembling ancestral mRNAs. Here, we analyzed the phenomenon of sustained translation of a leaderless mRNA in the presence of the antibiotic kasugamycin. Unexpected from the known in vitro effects of the drug, kasugamycin induced the formation of stable approximately 61S ribosomes in vivo, which were proficient in selectively translating leaderless mRNA. 61S particles are devoid of more than six proteins of the small subunit, including the functionally important proteins S1 and S12. The lack of these proteins could be reconciled with structural changes in the 16S rRNA. These studies provide in vivo evidence for the functionality of ribosomes devoid of multiple proteins and shed light on the evolutionary history of ribosomes.

AB - Translation of leaderless mRNAs, lacking ribosomal recruitment signals other than the 5'-terminal AUG-initiating codon, occurs in all three domains of life. Contemporary leaderless mRNAs may therefore be viewed as molecular fossils resembling ancestral mRNAs. Here, we analyzed the phenomenon of sustained translation of a leaderless mRNA in the presence of the antibiotic kasugamycin. Unexpected from the known in vitro effects of the drug, kasugamycin induced the formation of stable approximately 61S ribosomes in vivo, which were proficient in selectively translating leaderless mRNA. 61S particles are devoid of more than six proteins of the small subunit, including the functionally important proteins S1 and S12. The lack of these proteins could be reconciled with structural changes in the 16S rRNA. These studies provide in vivo evidence for the functionality of ribosomes devoid of multiple proteins and shed light on the evolutionary history of ribosomes.

M3 - SCORING: Zeitschriftenaufsatz

VL - 33

SP - 227

EP - 236

JO - MOL CELL

JF - MOL CELL

SN - 1097-2765

IS - 2

M1 - 2

ER -