An inactivating inward-rectifying K current present in prolactin cells from the pituitary of lactating rats
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An inactivating inward-rectifying K current present in prolactin cells from the pituitary of lactating rats. / Corrette, B J; Bauer, Christiane K.; Schwarz, J R.
In: J MEMBRANE BIOL, Vol. 150, No. 2, 1996, p. 185-195.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - An inactivating inward-rectifying K current present in prolactin cells from the pituitary of lactating rats
AU - Corrette, B J
AU - Bauer, Christiane K.
AU - Schwarz, J R
PY - 1996
Y1 - 1996
N2 - Primary cultures containing a high percentage of lactotrophs were obtained by dissociating the pituitary of rats following 14-18 days of lactation. Lactotrophs with a distinctive appearance were recorded after 1-35 days in vitro and identified by immunocytochemical staining for prolactin. Whole-cell voltage clamp measurements in isotonic KCl solution from a holding potential of -40 mV revealed the presence of inward-rectifying K currents with a time-dependent, Na(+)-independent inactivation at potentials negative to -60 mV. The time for complete inactivation was strikingly different between lactotrophs, varying between 1 sec and more than 5 sec at -120 mV, and was not related to time in culture. The reversal potential shifted 59 mV (25 degree C) for a tenfold change in external K+ concentration, demonstrating the selectivity of the channel for K+ over Na+. The inward-rectifying K current was blocked by 5 mM Ba2+ and partially blocked by 10 mM TEA. Chloramine-T (1 and 2 mM) produced a total block of the inward-rectifying K current in lactotrophs. Thyrotropin-releasing hormone (500 nM) significantly reduced the inward-rectifying K current in about half of the lactotrophs. This current is similar to the inward-rectifying K current previously characterized in clonal somatomammotrophic pituitary cells (GH3B6). The variability of the rate of inactivation of this current in lactotrophs and its responsiveness to TRH is discussed.
AB - Primary cultures containing a high percentage of lactotrophs were obtained by dissociating the pituitary of rats following 14-18 days of lactation. Lactotrophs with a distinctive appearance were recorded after 1-35 days in vitro and identified by immunocytochemical staining for prolactin. Whole-cell voltage clamp measurements in isotonic KCl solution from a holding potential of -40 mV revealed the presence of inward-rectifying K currents with a time-dependent, Na(+)-independent inactivation at potentials negative to -60 mV. The time for complete inactivation was strikingly different between lactotrophs, varying between 1 sec and more than 5 sec at -120 mV, and was not related to time in culture. The reversal potential shifted 59 mV (25 degree C) for a tenfold change in external K+ concentration, demonstrating the selectivity of the channel for K+ over Na+. The inward-rectifying K current was blocked by 5 mM Ba2+ and partially blocked by 10 mM TEA. Chloramine-T (1 and 2 mM) produced a total block of the inward-rectifying K current in lactotrophs. Thyrotropin-releasing hormone (500 nM) significantly reduced the inward-rectifying K current in about half of the lactotrophs. This current is similar to the inward-rectifying K current previously characterized in clonal somatomammotrophic pituitary cells (GH3B6). The variability of the rate of inactivation of this current in lactotrophs and its responsiveness to TRH is discussed.
U2 - 10.1007/s002329900043
DO - 10.1007/s002329900043
M3 - SCORING: Zeitschriftenaufsatz
VL - 150
SP - 185
EP - 195
JO - J MEMBRANE BIOL
JF - J MEMBRANE BIOL
SN - 0022-2631
IS - 2
ER -