An Early mtUPR: Redistribution of the Nuclear Transcription Factor Rox1 to Mitochondria Protects against Intramitochondrial Proteotoxic Aggregates
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An Early mtUPR: Redistribution of the Nuclear Transcription Factor Rox1 to Mitochondria Protects against Intramitochondrial Proteotoxic Aggregates. / Poveda-Huertes, Daniel; Matic, Stanka; Marada, Adinarayana; Habernig, Lukas; Licheva, Mariya; Myketin, Lisa; Gilsbach, Ralf; Tosal-Castano, Sergi; Papinski, Daniel; Mulica, Patrycja; Kretz, Oliver; Kücükköse, Cansu; Taskin, Asli Aras; Hein, Lutz; Kraft, Claudine; Büttner, Sabrina; Meisinger, Chris; Vögtle, F-Nora.
In: MOL CELL, Vol. 77, No. 1, 02.01.2020, p. 180-188.e9.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - An Early mtUPR: Redistribution of the Nuclear Transcription Factor Rox1 to Mitochondria Protects against Intramitochondrial Proteotoxic Aggregates
AU - Poveda-Huertes, Daniel
AU - Matic, Stanka
AU - Marada, Adinarayana
AU - Habernig, Lukas
AU - Licheva, Mariya
AU - Myketin, Lisa
AU - Gilsbach, Ralf
AU - Tosal-Castano, Sergi
AU - Papinski, Daniel
AU - Mulica, Patrycja
AU - Kretz, Oliver
AU - Kücükköse, Cansu
AU - Taskin, Asli Aras
AU - Hein, Lutz
AU - Kraft, Claudine
AU - Büttner, Sabrina
AU - Meisinger, Chris
AU - Vögtle, F-Nora
N1 - Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2020/1/2
Y1 - 2020/1/2
N2 - The mitochondrial proteome is built mainly by import of nuclear-encoded precursors, which are targeted mostly by cleavable presequences. Presequence processing upon import is essential for proteostasis and survival, but the consequences of dysfunctional protein maturation are unknown. We find that impaired presequence processing causes accumulation of precursors inside mitochondria that form aggregates, which escape degradation and unexpectedly do not cause cell death. Instead, cells survive via activation of a mitochondrial unfolded protein response (mtUPR)-like pathway that is triggered very early after precursor accumulation. In contrast to classical stress pathways, this immediate response maintains mitochondrial protein import, membrane potential, and translation through translocation of the nuclear HMG-box transcription factor Rox1 to mitochondria. Rox1 binds mtDNA and performs a TFAM-like function pivotal for transcription and translation. Induction of early mtUPR provides a reversible stress model to mechanistically dissect the initial steps in mtUPR pathways with the stressTFAM Rox1 as the first line of defense.
AB - The mitochondrial proteome is built mainly by import of nuclear-encoded precursors, which are targeted mostly by cleavable presequences. Presequence processing upon import is essential for proteostasis and survival, but the consequences of dysfunctional protein maturation are unknown. We find that impaired presequence processing causes accumulation of precursors inside mitochondria that form aggregates, which escape degradation and unexpectedly do not cause cell death. Instead, cells survive via activation of a mitochondrial unfolded protein response (mtUPR)-like pathway that is triggered very early after precursor accumulation. In contrast to classical stress pathways, this immediate response maintains mitochondrial protein import, membrane potential, and translation through translocation of the nuclear HMG-box transcription factor Rox1 to mitochondria. Rox1 binds mtDNA and performs a TFAM-like function pivotal for transcription and translation. Induction of early mtUPR provides a reversible stress model to mechanistically dissect the initial steps in mtUPR pathways with the stressTFAM Rox1 as the first line of defense.
KW - Cell Death/physiology
KW - Cell Nucleus/metabolism
KW - DNA, Mitochondrial/metabolism
KW - Membrane Potentials/physiology
KW - Mitochondria/metabolism
KW - Protein Biosynthesis/physiology
KW - Repressor Proteins/metabolism
KW - Saccharomyces cerevisiae Proteins/metabolism
KW - Saccharomyces cerevisiae/metabolism
KW - Transcription Factors/metabolism
KW - Transcription, Genetic/physiology
KW - Unfolded Protein Response/physiology
U2 - 10.1016/j.molcel.2019.09.026
DO - 10.1016/j.molcel.2019.09.026
M3 - SCORING: Journal article
C2 - 31630969
VL - 77
SP - 180-188.e9
JO - MOL CELL
JF - MOL CELL
SN - 1097-2765
IS - 1
ER -