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Amplification of different ColE1 plasmids in an Escherichia coli relA strain.

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Amplification of different ColE1 plasmids in an Escherichia coli relA strain. / Schroeter, A; Riethdorf, Sabine; Hecker, M.

In: J BASIC MICROB, Vol. 28, No. 8, 8, 1988, p. 553-555.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schroeter, A, Riethdorf, S & Hecker, M 1988, 'Amplification of different ColE1 plasmids in an Escherichia coli relA strain.', J BASIC MICROB, vol. 28, no. 8, 8, pp. 553-555. <http://www.ncbi.nlm.nih.gov/pubmed/3070008?dopt=Citation>

APA

Schroeter, A., Riethdorf, S., & Hecker, M. (1988). Amplification of different ColE1 plasmids in an Escherichia coli relA strain. J BASIC MICROB, 28(8), 553-555. [8]. http://www.ncbi.nlm.nih.gov/pubmed/3070008?dopt=Citation

Vancouver

Schroeter A, Riethdorf S, Hecker M. Amplification of different ColE1 plasmids in an Escherichia coli relA strain. J BASIC MICROB. 1988;28(8):553-555. 8.

Bibtex

@article{ef9e91f59c08403dac341edc7451ffba,
title = "Amplification of different ColE1 plasmids in an Escherichia coli relA strain.",
abstract = "Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColE1 related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see Boros et al. 1986). Rop+ and rop- plasmids show the same amplification rate under the used conditions.",
keywords = "*Gene Amplification, Escherichia coli/*genetics, *Plasmids, Amino Acids/metabolism, DNA Replication, DNA, Bacterial/*biosynthesis, *Gene Amplification, Escherichia coli/*genetics, *Plasmids, Amino Acids/metabolism, DNA Replication, DNA, Bacterial/*biosynthesis",
author = "A Schroeter and Sabine Riethdorf and M Hecker",
year = "1988",
language = "English",
volume = "28",
pages = "553--555",
number = "8",

}

RIS

TY - JOUR

T1 - Amplification of different ColE1 plasmids in an Escherichia coli relA strain.

AU - Schroeter, A

AU - Riethdorf, Sabine

AU - Hecker, M

PY - 1988

Y1 - 1988

N2 - Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColE1 related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see Boros et al. 1986). Rop+ and rop- plasmids show the same amplification rate under the used conditions.

AB - Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColE1 related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see Boros et al. 1986). Rop+ and rop- plasmids show the same amplification rate under the used conditions.

KW - Gene Amplification

KW - Escherichia coli/genetics

KW - Plasmids

KW - Amino Acids/metabolism

KW - DNA Replication

KW - DNA, Bacterial/biosynthesis

KW - Gene Amplification

KW - Escherichia coli/genetics

KW - Plasmids

KW - Amino Acids/metabolism

KW - DNA Replication

KW - DNA, Bacterial/biosynthesis

M3 - SCORING: Journal article

VL - 28

SP - 553

EP - 555

IS - 8

M1 - 8

ER -