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Altered lipid metabolism in the aging kidney identified by three layered omic analysis

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Altered lipid metabolism in the aging kidney identified by three layered omic analysis. / Braun, Fabian; Rinschen, Markus M; Bartels, Valerie; Frommolt, Peter; Habermann, Bianca; Hoeijmakers, Jan H J; Schumacher, Björn; Dollé, Martijn E T; Müller, Roman-Ulrich; Benzing, Thomas; Schermer, Bernhard; Kurschat, Christine E.

In: AGING-US, Vol. 8, No. 3, 03.2016, p. 441-57.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Braun, F, Rinschen, MM, Bartels, V, Frommolt, P, Habermann, B, Hoeijmakers, JHJ, Schumacher, B, Dollé, MET, Müller, R-U, Benzing, T, Schermer, B & Kurschat, CE 2016, 'Altered lipid metabolism in the aging kidney identified by three layered omic analysis', AGING-US, vol. 8, no. 3, pp. 441-57. https://doi.org/10.18632/aging.100900

APA

Braun, F., Rinschen, M. M., Bartels, V., Frommolt, P., Habermann, B., Hoeijmakers, J. H. J., Schumacher, B., Dollé, M. E. T., Müller, R-U., Benzing, T., Schermer, B., & Kurschat, C. E. (2016). Altered lipid metabolism in the aging kidney identified by three layered omic analysis. AGING-US, 8(3), 441-57. https://doi.org/10.18632/aging.100900

Vancouver

Braun F, Rinschen MM, Bartels V, Frommolt P, Habermann B, Hoeijmakers JHJ et al. Altered lipid metabolism in the aging kidney identified by three layered omic analysis. AGING-US. 2016 Mar;8(3):441-57. https://doi.org/10.18632/aging.100900

Bibtex

@article{45f1ffa9e0c04ed3aa7e698fc7d5968e,
title = "Altered lipid metabolism in the aging kidney identified by three layered omic analysis",
abstract = "Aging-associated diseases and their comorbidities affect the life of a constantly growing proportion of the population in developed countries. At the center of these comorbidities are changes of kidney structure and function as age-related chronic kidney disease predisposes to the development of cardiovascular diseases such as stroke, myocardial infarction or heart failure. To detect molecular mechanisms involved in kidney aging, we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by transcriptomic, proteomic and targeted lipidomic methodologies. Interestingly, transcriptome and proteome analyses revealed differential expression of genes primarily involved in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides with age. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16. In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease.",
keywords = "Acid Ceramidase, Aging, Animals, Ceramides, Kidney, Lipid Metabolism, Mass Spectrometry, Mice, Inbred C57BL, Phenotype, Phospholipids, Proteomics, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Fabian Braun and Rinschen, {Markus M} and Valerie Bartels and Peter Frommolt and Bianca Habermann and Hoeijmakers, {Jan H J} and Bj{\"o}rn Schumacher and Doll{\'e}, {Martijn E T} and Roman-Ulrich M{\"u}ller and Thomas Benzing and Bernhard Schermer and Kurschat, {Christine E}",
year = "2016",
month = mar,
doi = "10.18632/aging.100900",
language = "English",
volume = "8",
pages = "441--57",
journal = "AGING-US",
issn = "1945-4589",
publisher = "US Administration on Aging",
number = "3",

}

RIS

TY - JOUR

T1 - Altered lipid metabolism in the aging kidney identified by three layered omic analysis

AU - Braun, Fabian

AU - Rinschen, Markus M

AU - Bartels, Valerie

AU - Frommolt, Peter

AU - Habermann, Bianca

AU - Hoeijmakers, Jan H J

AU - Schumacher, Björn

AU - Dollé, Martijn E T

AU - Müller, Roman-Ulrich

AU - Benzing, Thomas

AU - Schermer, Bernhard

AU - Kurschat, Christine E

PY - 2016/3

Y1 - 2016/3

N2 - Aging-associated diseases and their comorbidities affect the life of a constantly growing proportion of the population in developed countries. At the center of these comorbidities are changes of kidney structure and function as age-related chronic kidney disease predisposes to the development of cardiovascular diseases such as stroke, myocardial infarction or heart failure. To detect molecular mechanisms involved in kidney aging, we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by transcriptomic, proteomic and targeted lipidomic methodologies. Interestingly, transcriptome and proteome analyses revealed differential expression of genes primarily involved in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides with age. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16. In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease.

AB - Aging-associated diseases and their comorbidities affect the life of a constantly growing proportion of the population in developed countries. At the center of these comorbidities are changes of kidney structure and function as age-related chronic kidney disease predisposes to the development of cardiovascular diseases such as stroke, myocardial infarction or heart failure. To detect molecular mechanisms involved in kidney aging, we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by transcriptomic, proteomic and targeted lipidomic methodologies. Interestingly, transcriptome and proteome analyses revealed differential expression of genes primarily involved in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides with age. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16. In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease.

KW - Acid Ceramidase

KW - Aging

KW - Animals

KW - Ceramides

KW - Kidney

KW - Lipid Metabolism

KW - Mass Spectrometry

KW - Mice, Inbred C57BL

KW - Phenotype

KW - Phospholipids

KW - Proteomics

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.18632/aging.100900

DO - 10.18632/aging.100900

M3 - SCORING: Journal article

C2 - 26886165

VL - 8

SP - 441

EP - 457

JO - AGING-US

JF - AGING-US

SN - 1945-4589

IS - 3

ER -