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Alterations of the bile microbiome in primary sclerosing cholangitis

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Alterations of the bile microbiome in primary sclerosing cholangitis. / Liwinski, Timur; Zenouzi, Roman; John, Clara; Ehlken, Hanno; Rühlemann, Malte C; Bang, Corinna; Groth, Stefan; Lieb, Wolfgang; Kantowski, Marcus; Andersen, Nils; Schachschal, Guido; Karlsen, Tom H; Hov, Johannes R; Rösch, Thomas; Lohse, Ansgar W; Heeren, Joerg; Franke, Andre; Schramm, Christoph.

In: GUT, Vol. 69, No. 4, 04.2020, p. 665-672.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Liwinski, T, Zenouzi, R, John, C, Ehlken, H, Rühlemann, MC, Bang, C, Groth, S, Lieb, W, Kantowski, M, Andersen, N, Schachschal, G, Karlsen, TH, Hov, JR, Rösch, T, Lohse, AW, Heeren, J, Franke, A & Schramm, C 2020, 'Alterations of the bile microbiome in primary sclerosing cholangitis', GUT, vol. 69, no. 4, pp. 665-672. https://doi.org/10.1136/gutjnl-2019-318416

APA

Liwinski, T., Zenouzi, R., John, C., Ehlken, H., Rühlemann, M. C., Bang, C., Groth, S., Lieb, W., Kantowski, M., Andersen, N., Schachschal, G., Karlsen, T. H., Hov, J. R., Rösch, T., Lohse, A. W., Heeren, J., Franke, A., & Schramm, C. (2020). Alterations of the bile microbiome in primary sclerosing cholangitis. GUT, 69(4), 665-672. https://doi.org/10.1136/gutjnl-2019-318416

Vancouver

Liwinski T, Zenouzi R, John C, Ehlken H, Rühlemann MC, Bang C et al. Alterations of the bile microbiome in primary sclerosing cholangitis. GUT. 2020 Apr;69(4):665-672. https://doi.org/10.1136/gutjnl-2019-318416

Bibtex

@article{597fc62fabd5489f988180b1779afddc,
title = "Alterations of the bile microbiome in primary sclerosing cholangitis",
abstract = "BACKGROUND: Patients with primary sclerosing cholangitis (PSC) display an altered colonic microbiome compared with healthy controls. However, little is known on the bile duct microbiome and its interplay with bile acid metabolism in PSC.METHODS: Patients with PSC (n=43) and controls without sclerosing cholangitis (n=22) requiring endoscopic retrograde cholangiography were included prospectively. Leading indications in controls were sporadic choledocholithiasis and papillary adenoma. A total of 260 biospecimens were collected from the oral cavity, duodenal fluid and mucosa and ductal bile. Microbiomes of the upper alimentary tract and ductal bile were profiled by sequencing the 16S-rRNA-encoding gene (V1-V2). Bile fluid bile acid composition was measured by high-performance liquid chromatography mass spectrometry and validated in an external cohort (n=20).RESULTS: The bile fluid harboured a diverse microbiome that was distinct from the oral cavity, the duodenal fluid and duodenal mucosa communities. The upper alimentary tract microbiome differed between PSC patients and controls. However, the strongest differences between PSC patients and controls were observed in the ductal bile fluid, including reduced biodiversity (Shannon entropy, p=0.0127) and increase of pathogen Enterococcus faecalis (FDR=4.18×10-5) in PSC. Enterococcus abundance in ductal bile was strongly correlated with concentration of the noxious secondary bile acid taurolithocholic acid (r=0.60, p=0.0021).CONCLUSION: PSC is characterised by an altered microbiome of the upper alimentary tract and bile ducts. Biliary dysbiosis is linked with increased concentrations of the proinflammatory and potentially cancerogenic agent taurolithocholic acid.",
keywords = "Adult, Aged, Aged, 80 and over, Bile Ducts/microbiology, Bile/microbiology, Case-Control Studies, Cholangitis, Sclerosing/microbiology, Cohort Studies, Duodenum/microbiology, Dysbiosis/complications, Female, Humans, Male, Microbiota, Middle Aged, Mouth Mucosa/microbiology, Young Adult",
author = "Timur Liwinski and Roman Zenouzi and Clara John and Hanno Ehlken and R{\"u}hlemann, {Malte C} and Corinna Bang and Stefan Groth and Wolfgang Lieb and Marcus Kantowski and Nils Andersen and Guido Schachschal and Karlsen, {Tom H} and Hov, {Johannes R} and Thomas R{\"o}sch and Lohse, {Ansgar W} and Joerg Heeren and Andre Franke and Christoph Schramm",
note = "{\textcopyright} Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.",
year = "2020",
month = apr,
doi = "10.1136/gutjnl-2019-318416",
language = "English",
volume = "69",
pages = "665--672",
journal = "GUT",
issn = "0017-5749",
publisher = "BMJ PUBLISHING GROUP",
number = "4",

}

RIS

TY - JOUR

T1 - Alterations of the bile microbiome in primary sclerosing cholangitis

AU - Liwinski, Timur

AU - Zenouzi, Roman

AU - John, Clara

AU - Ehlken, Hanno

AU - Rühlemann, Malte C

AU - Bang, Corinna

AU - Groth, Stefan

AU - Lieb, Wolfgang

AU - Kantowski, Marcus

AU - Andersen, Nils

AU - Schachschal, Guido

AU - Karlsen, Tom H

AU - Hov, Johannes R

AU - Rösch, Thomas

AU - Lohse, Ansgar W

AU - Heeren, Joerg

AU - Franke, Andre

AU - Schramm, Christoph

N1 - © Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2020/4

Y1 - 2020/4

N2 - BACKGROUND: Patients with primary sclerosing cholangitis (PSC) display an altered colonic microbiome compared with healthy controls. However, little is known on the bile duct microbiome and its interplay with bile acid metabolism in PSC.METHODS: Patients with PSC (n=43) and controls without sclerosing cholangitis (n=22) requiring endoscopic retrograde cholangiography were included prospectively. Leading indications in controls were sporadic choledocholithiasis and papillary adenoma. A total of 260 biospecimens were collected from the oral cavity, duodenal fluid and mucosa and ductal bile. Microbiomes of the upper alimentary tract and ductal bile were profiled by sequencing the 16S-rRNA-encoding gene (V1-V2). Bile fluid bile acid composition was measured by high-performance liquid chromatography mass spectrometry and validated in an external cohort (n=20).RESULTS: The bile fluid harboured a diverse microbiome that was distinct from the oral cavity, the duodenal fluid and duodenal mucosa communities. The upper alimentary tract microbiome differed between PSC patients and controls. However, the strongest differences between PSC patients and controls were observed in the ductal bile fluid, including reduced biodiversity (Shannon entropy, p=0.0127) and increase of pathogen Enterococcus faecalis (FDR=4.18×10-5) in PSC. Enterococcus abundance in ductal bile was strongly correlated with concentration of the noxious secondary bile acid taurolithocholic acid (r=0.60, p=0.0021).CONCLUSION: PSC is characterised by an altered microbiome of the upper alimentary tract and bile ducts. Biliary dysbiosis is linked with increased concentrations of the proinflammatory and potentially cancerogenic agent taurolithocholic acid.

AB - BACKGROUND: Patients with primary sclerosing cholangitis (PSC) display an altered colonic microbiome compared with healthy controls. However, little is known on the bile duct microbiome and its interplay with bile acid metabolism in PSC.METHODS: Patients with PSC (n=43) and controls without sclerosing cholangitis (n=22) requiring endoscopic retrograde cholangiography were included prospectively. Leading indications in controls were sporadic choledocholithiasis and papillary adenoma. A total of 260 biospecimens were collected from the oral cavity, duodenal fluid and mucosa and ductal bile. Microbiomes of the upper alimentary tract and ductal bile were profiled by sequencing the 16S-rRNA-encoding gene (V1-V2). Bile fluid bile acid composition was measured by high-performance liquid chromatography mass spectrometry and validated in an external cohort (n=20).RESULTS: The bile fluid harboured a diverse microbiome that was distinct from the oral cavity, the duodenal fluid and duodenal mucosa communities. The upper alimentary tract microbiome differed between PSC patients and controls. However, the strongest differences between PSC patients and controls were observed in the ductal bile fluid, including reduced biodiversity (Shannon entropy, p=0.0127) and increase of pathogen Enterococcus faecalis (FDR=4.18×10-5) in PSC. Enterococcus abundance in ductal bile was strongly correlated with concentration of the noxious secondary bile acid taurolithocholic acid (r=0.60, p=0.0021).CONCLUSION: PSC is characterised by an altered microbiome of the upper alimentary tract and bile ducts. Biliary dysbiosis is linked with increased concentrations of the proinflammatory and potentially cancerogenic agent taurolithocholic acid.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Bile Ducts/microbiology

KW - Bile/microbiology

KW - Case-Control Studies

KW - Cholangitis, Sclerosing/microbiology

KW - Cohort Studies

KW - Duodenum/microbiology

KW - Dysbiosis/complications

KW - Female

KW - Humans

KW - Male

KW - Microbiota

KW - Middle Aged

KW - Mouth Mucosa/microbiology

KW - Young Adult

U2 - 10.1136/gutjnl-2019-318416

DO - 10.1136/gutjnl-2019-318416

M3 - SCORING: Journal article

C2 - 31243055

VL - 69

SP - 665

EP - 672

JO - GUT

JF - GUT

SN - 0017-5749

IS - 4

ER -