Alpha- and beta-tubulin isotypes are differentially expressed during brain development
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Alpha- and beta-tubulin isotypes are differentially expressed during brain development. / Hausrat, Torben J; Radwitz, Jennifer; Lombino, Franco L; Breiden, Petra; Kneussel, Matthias.
In: DEV NEUROBIOL, Vol. 81, No. 3, 04.2021, p. 333-350.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Alpha- and beta-tubulin isotypes are differentially expressed during brain development
AU - Hausrat, Torben J
AU - Radwitz, Jennifer
AU - Lombino, Franco L
AU - Breiden, Petra
AU - Kneussel, Matthias
N1 - © 2020 The Authors. Developmental Neurobiology published by Wiley Periodicals LLC.
PY - 2021/4
Y1 - 2021/4
N2 - Alpha- and beta-tubulin dimers polymerize into protofilaments that associate laterally to constitute a hollow tube, the microtubule. A dynamic network of interlinking filaments forms the microtubule cytoskeleton, which maintains the structure of cells and is key to various cellular processes including cell division, cell migration, and intracellular transport. Individual microtubules have an identity that depends on the differential integration of specific alpha- and beta-tubulin isotypes and is further specified by a variety of posttranslational modifications (PTMs). It is barely understood to which extent neighboring microtubules differ in their tubulin composition or whether specific tubulin isotypes cluster along the polymer. Furthermore, our knowledge about the spatio-temporal expression patterns of tubulin isotypes is limited, not at least due to the lack of antibodies or antibody cross-reactivities. Here, we asked which alpha- and beta-tubulin mRNAs and proteins are expressed in developing hippocampal neuron cultures and ex vivo brain tissue lysates. Using heterologous expression of GFP-tubulin fusion proteins, we systematically tested antibody-specificities against various tubulin isotypes. Our data provide quantitative information about tubulin expression levels in the mouse brain and classify tubulin isotypes during pre- and postnatal development.
AB - Alpha- and beta-tubulin dimers polymerize into protofilaments that associate laterally to constitute a hollow tube, the microtubule. A dynamic network of interlinking filaments forms the microtubule cytoskeleton, which maintains the structure of cells and is key to various cellular processes including cell division, cell migration, and intracellular transport. Individual microtubules have an identity that depends on the differential integration of specific alpha- and beta-tubulin isotypes and is further specified by a variety of posttranslational modifications (PTMs). It is barely understood to which extent neighboring microtubules differ in their tubulin composition or whether specific tubulin isotypes cluster along the polymer. Furthermore, our knowledge about the spatio-temporal expression patterns of tubulin isotypes is limited, not at least due to the lack of antibodies or antibody cross-reactivities. Here, we asked which alpha- and beta-tubulin mRNAs and proteins are expressed in developing hippocampal neuron cultures and ex vivo brain tissue lysates. Using heterologous expression of GFP-tubulin fusion proteins, we systematically tested antibody-specificities against various tubulin isotypes. Our data provide quantitative information about tubulin expression levels in the mouse brain and classify tubulin isotypes during pre- and postnatal development.
U2 - 10.1002/dneu.22745
DO - 10.1002/dneu.22745
M3 - SCORING: Journal article
C2 - 32293117
VL - 81
SP - 333
EP - 350
JO - DEV NEUROBIOL
JF - DEV NEUROBIOL
SN - 1932-8451
IS - 3
ER -
