ALCAM (CD166) expression and serum levels in pancreatic cancer
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ALCAM (CD166) expression and serum levels in pancreatic cancer. / Tachezy, Michael; Zander, Hilke; Marx, Andreas H; Stahl, Phillip R; Gebauer, Florian; Izbicki, Jakob R; Bockhorn, Maximilian.
In: PLOS ONE, Vol. 7, No. 6, 6, 01.01.2012, p. e39018.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - ALCAM (CD166) expression and serum levels in pancreatic cancer
AU - Tachezy, Michael
AU - Zander, Hilke
AU - Marx, Andreas H
AU - Stahl, Phillip R
AU - Gebauer, Florian
AU - Izbicki, Jakob R
AU - Bockhorn, Maximilian
PY - 2012/1/1
Y1 - 2012/1/1
N2 - BACKGROUND: This study was conducted to evaluate the expression of the activated leukocyte cell adhesion molecule (ALCAM) in pancreatic cancer (PAC) and to determine whether or not the ectodomain shedding of ALCAM (s-ALCAM) could serve as a biomarker in the peripheral blood of PAC patients.MATERIAL AND METHODS: Tissue specimens and blood sera of patients with PAC (n = 264 and n = 116, respectively) and the sera of 115 patients with chronic pancreatitis (CP) were analyzed via ALCAM immunohistochemistry and s-ALCAM ELISA tests. Results were correlated with clinical, histopathological, and patient survival data (Chi-square test, Kaplan-Meier analysis, log-rank test, respectively).RESULTS: ALCAM was expressed in the majority of PAC lesions. Immunohistochemistry and serum ELISA tests revealed no association between ALCAM expression in primary tumors or s-ALCAM and clinical or histopathological data. Neither ALCAM nor s-ALCAM showed a significant impact regarding overall survival (p = 0.261 and p = 0.660, respectively). S-ALCAM serum levels were significantly elevated compared to the sera of CP patients (p<0.001). The sensitivity of s-ALCAM in detecting PAC was 58.6% at a specificity of 73.9% (AUC = 0.69).CONCLUSIONS: ALCAM is expressed in the majority of PAC lesions, but statistical analysis revealed no association with clinical or pathological data. Although significantly elevated in patients with PAC, the sensitivity and specificity of the s-ALCAM serum quantification test was low. Therefore, its potential as a novel diagnostic marker for PAC remains elusive and further investigations are required.
AB - BACKGROUND: This study was conducted to evaluate the expression of the activated leukocyte cell adhesion molecule (ALCAM) in pancreatic cancer (PAC) and to determine whether or not the ectodomain shedding of ALCAM (s-ALCAM) could serve as a biomarker in the peripheral blood of PAC patients.MATERIAL AND METHODS: Tissue specimens and blood sera of patients with PAC (n = 264 and n = 116, respectively) and the sera of 115 patients with chronic pancreatitis (CP) were analyzed via ALCAM immunohistochemistry and s-ALCAM ELISA tests. Results were correlated with clinical, histopathological, and patient survival data (Chi-square test, Kaplan-Meier analysis, log-rank test, respectively).RESULTS: ALCAM was expressed in the majority of PAC lesions. Immunohistochemistry and serum ELISA tests revealed no association between ALCAM expression in primary tumors or s-ALCAM and clinical or histopathological data. Neither ALCAM nor s-ALCAM showed a significant impact regarding overall survival (p = 0.261 and p = 0.660, respectively). S-ALCAM serum levels were significantly elevated compared to the sera of CP patients (p<0.001). The sensitivity of s-ALCAM in detecting PAC was 58.6% at a specificity of 73.9% (AUC = 0.69).CONCLUSIONS: ALCAM is expressed in the majority of PAC lesions, but statistical analysis revealed no association with clinical or pathological data. Although significantly elevated in patients with PAC, the sensitivity and specificity of the s-ALCAM serum quantification test was low. Therefore, its potential as a novel diagnostic marker for PAC remains elusive and further investigations are required.
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Immunohistochemistry
KW - Enzyme-Linked Immunosorbent Assay
KW - Antigens, CD/blood/metabolism
KW - Cell Adhesion Molecules, Neuronal/blood/metabolism
KW - Fetal Proteins/blood/metabolism
KW - Pancreatic Neoplasms/blood/metabolism
KW - Pancreatitis, Chronic/blood/metabolism
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Immunohistochemistry
KW - Enzyme-Linked Immunosorbent Assay
KW - Antigens, CD/blood/metabolism
KW - Cell Adhesion Molecules, Neuronal/blood/metabolism
KW - Fetal Proteins/blood/metabolism
KW - Pancreatic Neoplasms/blood/metabolism
KW - Pancreatitis, Chronic/blood/metabolism
U2 - 10.1371/journal.pone.0039018
DO - 10.1371/journal.pone.0039018
M3 - SCORING: Journal article
C2 - 22745698
VL - 7
SP - e39018
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 6
M1 - 6
ER -
