Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model
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Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model. / Fiedorowicz, Michal; Rejdak, Robert; Schuettauf, Frank; Wozniak, Michal; Grieb, Pawel; Thaler, Sebastian.
In: FOLIA NEUROPATHOL, Vol. 52, No. 3, 2014, p. 291-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Age-dependent neuroprotection of retinal ganglion cells by tempol-C8 acyl ester in a rat NMDA toxicity model
AU - Fiedorowicz, Michal
AU - Rejdak, Robert
AU - Schuettauf, Frank
AU - Wozniak, Michal
AU - Grieb, Pawel
AU - Thaler, Sebastian
PY - 2014
Y1 - 2014
N2 - BACKGROUND: The efficacy of tempol and its acyl derivative tempol-C8 as retinoprotective agents was compared in a rat model of NMDA-induced retinal ganglion cell (RGC) damage.MATERIAL AND METHODS: Tempol or tempol-C8 in different doses was administered intraperitoneally to 6 weeks old (pre-adolescent) and 9-10 weeks old (young adult) rats before and after an intravitreous NMDA injection. Retinal ganglion cell were retrogradely labeled with the fluorescent tracer hydroxystilbamidine and RGC counting was performed on retinal flatmounts.RESULTS: Intravitreal NMDA reduced RGC counts by about 90%, independently of age < 0.001). In pre-adolescent animals tempol-C8, but not tempol unmodified, showed a significant, dose-dependent RGC rescue effect, with peak activity at 5.8 µmol/kg (p < 0.001). In young adult animals, however, no neuroprotective effect was found for either tempol or tempol-C8.CONCLUSIONS: In contrast to tempol itself, tempol-C8 acyl ester was neuroprotective in pre-adolescent rats in the NMDA- induced RGC damage model. Therefore, neuroprotection by tempol acyl esters seems to be superior to that of tempol under certain conditions.
AB - BACKGROUND: The efficacy of tempol and its acyl derivative tempol-C8 as retinoprotective agents was compared in a rat model of NMDA-induced retinal ganglion cell (RGC) damage.MATERIAL AND METHODS: Tempol or tempol-C8 in different doses was administered intraperitoneally to 6 weeks old (pre-adolescent) and 9-10 weeks old (young adult) rats before and after an intravitreous NMDA injection. Retinal ganglion cell were retrogradely labeled with the fluorescent tracer hydroxystilbamidine and RGC counting was performed on retinal flatmounts.RESULTS: Intravitreal NMDA reduced RGC counts by about 90%, independently of age < 0.001). In pre-adolescent animals tempol-C8, but not tempol unmodified, showed a significant, dose-dependent RGC rescue effect, with peak activity at 5.8 µmol/kg (p < 0.001). In young adult animals, however, no neuroprotective effect was found for either tempol or tempol-C8.CONCLUSIONS: In contrast to tempol itself, tempol-C8 acyl ester was neuroprotective in pre-adolescent rats in the NMDA- induced RGC damage model. Therefore, neuroprotection by tempol acyl esters seems to be superior to that of tempol under certain conditions.
KW - Aging
KW - Animals
KW - Cyclic N-Oxides/pharmacology
KW - Disease Models, Animal
KW - Excitatory Amino Acid Agonists/toxicity
KW - N-Methylaspartate/toxicity
KW - Neuroprotective Agents/pharmacology
KW - Rats
KW - Retinal Ganglion Cells/drug effects
KW - Spin Labels
U2 - 10.5114/fn.2014.45570
DO - 10.5114/fn.2014.45570
M3 - SCORING: Journal article
C2 - 25310740
VL - 52
SP - 291
EP - 297
JO - FOLIA NEUROPATHOL
JF - FOLIA NEUROPATHOL
SN - 1641-4640
IS - 3
ER -
