Adoptive transfer of donor-derived immunity by liver transplantation
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Adoptive transfer of donor-derived immunity by liver transplantation : a potential avenue to prevent hepatitis B virus reinfection. / Dahmen, U; Dirsch, O; Gu, Y L; Polywka, S; Doebel, L; Shen, K; Broelsch, C E.
In: J VIRAL HEPATITIS, Vol. 10, No. 1, 01.2003, p. 31-6.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Adoptive transfer of donor-derived immunity by liver transplantation
T2 - a potential avenue to prevent hepatitis B virus reinfection
AU - Dahmen, U
AU - Dirsch, O
AU - Gu, Y L
AU - Polywka, S
AU - Doebel, L
AU - Shen, K
AU - Broelsch, C E
PY - 2003/1
Y1 - 2003/1
N2 - Immunity to hepatitis B has been successfully transferred by bone marrow transplantation, but has also occurred after liver transplantation (LTx). This study was designed to analyse the influence of alloreactivity and immunosuppression, on the efficacy of adoptive immune transfer to hepatitis B by liver transplantation. Orthotopic LTx (n = 34) were performed in three rat strain combinations representing different genetic constellations. Donors had been vaccinated twice with recombinant hepatitis B surface antigen while recipients were unimmunized. Half of the allogeneic recipients were immunosuppressed with cyclosporin A. All animals were monitored weekly for the presence of anti-hepatitis B surface antibodies (anti-HBs). Effective anti-HBs titres were detected in 85% (29/34) of liver recipients and lasted from 2 to 10 weeks. Donor titre above >15 000 mIU/mL ensured a 100% seroconversion rate in the recipients. The maximal anti-HBs titre in recipients represented 0.06% approximately 0.76% of the donor titre. Rejection reduced the adoptive immune transfer, which was protected by immunosuppression. These observations suggest that transfer of functionally active donor lymphocytes, deriving from the graft, contributed to the donor-derived immune response in the recipient. Further studies to augment the donor-derived immune response are warranted to ensure a therapeutic effect for the recipient at risk of reinfection.
AB - Immunity to hepatitis B has been successfully transferred by bone marrow transplantation, but has also occurred after liver transplantation (LTx). This study was designed to analyse the influence of alloreactivity and immunosuppression, on the efficacy of adoptive immune transfer to hepatitis B by liver transplantation. Orthotopic LTx (n = 34) were performed in three rat strain combinations representing different genetic constellations. Donors had been vaccinated twice with recombinant hepatitis B surface antigen while recipients were unimmunized. Half of the allogeneic recipients were immunosuppressed with cyclosporin A. All animals were monitored weekly for the presence of anti-hepatitis B surface antibodies (anti-HBs). Effective anti-HBs titres were detected in 85% (29/34) of liver recipients and lasted from 2 to 10 weeks. Donor titre above >15 000 mIU/mL ensured a 100% seroconversion rate in the recipients. The maximal anti-HBs titre in recipients represented 0.06% approximately 0.76% of the donor titre. Rejection reduced the adoptive immune transfer, which was protected by immunosuppression. These observations suggest that transfer of functionally active donor lymphocytes, deriving from the graft, contributed to the donor-derived immune response in the recipient. Further studies to augment the donor-derived immune response are warranted to ensure a therapeutic effect for the recipient at risk of reinfection.
KW - Animals
KW - Bone Marrow Transplantation
KW - Disease Models, Animal
KW - Graft Survival
KW - Hepatitis B
KW - Hepatitis B Antibodies
KW - Hepatitis B Surface Antigens
KW - Hepatitis B Vaccines
KW - Immunosuppression
KW - Immunotherapy, Adoptive
KW - Liver Transplantation
KW - Male
KW - Rats
KW - Rats, Inbred ACI
KW - Rats, Inbred BN
KW - Rats, Inbred Lew
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 12558909
VL - 10
SP - 31
EP - 36
JO - J VIRAL HEPATITIS
JF - J VIRAL HEPATITIS
SN - 1352-0504
IS - 1
ER -