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Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats

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Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. / Lange, Claudia; Tögel, Florian; Ittrich, Harald; Clayton, Frederic; Nolte-Ernsting, Claus; Zander, Axel R; Westenfelder, Christof.

In: KIDNEY INT, Vol. 68, No. 4, 01.10.2005, p. 1613-7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lange, C, Tögel, F, Ittrich, H, Clayton, F, Nolte-Ernsting, C, Zander, AR & Westenfelder, C 2005, 'Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats', KIDNEY INT, vol. 68, no. 4, pp. 1613-7. https://doi.org/10.1111/j.1523-1755.2005.00573.x

APA

Lange, C., Tögel, F., Ittrich, H., Clayton, F., Nolte-Ernsting, C., Zander, A. R., & Westenfelder, C. (2005). Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. KIDNEY INT, 68(4), 1613-7. https://doi.org/10.1111/j.1523-1755.2005.00573.x

Vancouver

Lange C, Tögel F, Ittrich H, Clayton F, Nolte-Ernsting C, Zander AR et al. Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. KIDNEY INT. 2005 Oct 1;68(4):1613-7. https://doi.org/10.1111/j.1523-1755.2005.00573.x

Bibtex

@article{5375641a1625414b8697c6933bd0fa8f,
title = "Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats",
abstract = "BACKGROUND: Adult stem cells are promising for the development of novel therapies in regenerative medicine. Acute renal failure (ARF) remains a frequent clinical complication, associated with an unacceptably high mortality rate, in large part due to the ineffectiveness of currently available therapies. The aim of this study was, therefore, to evaluate the therapeutic effectiveness of bone marrow-derived mesenchymal stem cells in a rat model of ischemia/reperfusion (I/R) ARF.METHODS: We used a common I/R model in rats to induce ARF by clamping both renal pedicles for 40 minutes. Mesenchymal stem cells were iron-dextran-labeled for in vivo tracking studies by magnetic resonance imaging (MRI) and kidneys were imaged for mesenchymal stem cells immediately after infusion and at day 3 after ARF. Renal injury was scored on day 3 and cells were additionally tracked by Prussian blue staining.RESULTS: We show in I/R-induced ARF in rats, modeling the most common form of clinical ARF, that infusion of mesenchymal stem cells enhances recovery of renal function. Mesenchymal stem cells were found to be located in the kidney cortex after injection, as demonstrated by MRI. Mesenchymal stem cells-treated animals had both significantly better renal function on days 2 and 3 and better injury scores at day 3 after ARF. Histologically, mesenchymal stem cells were predominantly located in glomerular capillaries, while tubules showed no iron labeling, indicating absent tubular transdifferentiation.CONCLUSION: We conclude that the highly renoprotective capacity of mesenchymal stem cells opens the possibility for a cell-based paradigm shift in the treatment of I/R ARF.",
keywords = "Acute Kidney Injury, Animals, Coloring Agents, Ferrocyanides, Graft Survival, Iron-Dextran Complex, Kidney, Magnetic Resonance Imaging, Male, Mesoderm, Rats, Rats, Sprague-Dawley, Recovery of Function, Reperfusion Injury, Stem Cell Transplantation",
author = "Claudia Lange and Florian T{\"o}gel and Harald Ittrich and Frederic Clayton and Claus Nolte-Ernsting and Zander, {Axel R} and Christof Westenfelder",
year = "2005",
month = oct,
day = "1",
doi = "10.1111/j.1523-1755.2005.00573.x",
language = "English",
volume = "68",
pages = "1613--7",
journal = "KIDNEY INT",
issn = "0085-2538",
publisher = "NATURE PUBLISHING GROUP",
number = "4",

}

RIS

TY - JOUR

T1 - Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats

AU - Lange, Claudia

AU - Tögel, Florian

AU - Ittrich, Harald

AU - Clayton, Frederic

AU - Nolte-Ernsting, Claus

AU - Zander, Axel R

AU - Westenfelder, Christof

PY - 2005/10/1

Y1 - 2005/10/1

N2 - BACKGROUND: Adult stem cells are promising for the development of novel therapies in regenerative medicine. Acute renal failure (ARF) remains a frequent clinical complication, associated with an unacceptably high mortality rate, in large part due to the ineffectiveness of currently available therapies. The aim of this study was, therefore, to evaluate the therapeutic effectiveness of bone marrow-derived mesenchymal stem cells in a rat model of ischemia/reperfusion (I/R) ARF.METHODS: We used a common I/R model in rats to induce ARF by clamping both renal pedicles for 40 minutes. Mesenchymal stem cells were iron-dextran-labeled for in vivo tracking studies by magnetic resonance imaging (MRI) and kidneys were imaged for mesenchymal stem cells immediately after infusion and at day 3 after ARF. Renal injury was scored on day 3 and cells were additionally tracked by Prussian blue staining.RESULTS: We show in I/R-induced ARF in rats, modeling the most common form of clinical ARF, that infusion of mesenchymal stem cells enhances recovery of renal function. Mesenchymal stem cells were found to be located in the kidney cortex after injection, as demonstrated by MRI. Mesenchymal stem cells-treated animals had both significantly better renal function on days 2 and 3 and better injury scores at day 3 after ARF. Histologically, mesenchymal stem cells were predominantly located in glomerular capillaries, while tubules showed no iron labeling, indicating absent tubular transdifferentiation.CONCLUSION: We conclude that the highly renoprotective capacity of mesenchymal stem cells opens the possibility for a cell-based paradigm shift in the treatment of I/R ARF.

AB - BACKGROUND: Adult stem cells are promising for the development of novel therapies in regenerative medicine. Acute renal failure (ARF) remains a frequent clinical complication, associated with an unacceptably high mortality rate, in large part due to the ineffectiveness of currently available therapies. The aim of this study was, therefore, to evaluate the therapeutic effectiveness of bone marrow-derived mesenchymal stem cells in a rat model of ischemia/reperfusion (I/R) ARF.METHODS: We used a common I/R model in rats to induce ARF by clamping both renal pedicles for 40 minutes. Mesenchymal stem cells were iron-dextran-labeled for in vivo tracking studies by magnetic resonance imaging (MRI) and kidneys were imaged for mesenchymal stem cells immediately after infusion and at day 3 after ARF. Renal injury was scored on day 3 and cells were additionally tracked by Prussian blue staining.RESULTS: We show in I/R-induced ARF in rats, modeling the most common form of clinical ARF, that infusion of mesenchymal stem cells enhances recovery of renal function. Mesenchymal stem cells were found to be located in the kidney cortex after injection, as demonstrated by MRI. Mesenchymal stem cells-treated animals had both significantly better renal function on days 2 and 3 and better injury scores at day 3 after ARF. Histologically, mesenchymal stem cells were predominantly located in glomerular capillaries, while tubules showed no iron labeling, indicating absent tubular transdifferentiation.CONCLUSION: We conclude that the highly renoprotective capacity of mesenchymal stem cells opens the possibility for a cell-based paradigm shift in the treatment of I/R ARF.

KW - Acute Kidney Injury

KW - Animals

KW - Coloring Agents

KW - Ferrocyanides

KW - Graft Survival

KW - Iron-Dextran Complex

KW - Kidney

KW - Magnetic Resonance Imaging

KW - Male

KW - Mesoderm

KW - Rats

KW - Rats, Sprague-Dawley

KW - Recovery of Function

KW - Reperfusion Injury

KW - Stem Cell Transplantation

U2 - 10.1111/j.1523-1755.2005.00573.x

DO - 10.1111/j.1523-1755.2005.00573.x

M3 - SCORING: Journal article

C2 - 16164638

VL - 68

SP - 1613

EP - 1617

JO - KIDNEY INT

JF - KIDNEY INT

SN - 0085-2538

IS - 4

ER -