Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go.
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Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go. / Hinsch, K D; Rosenthal, W; Spicher, K; Binder, Thomas; Gausepohl, H; Frank, R; Schultz, G; Joost, H G.
In: FEBS LETT, Vol. 238, No. 1, 1, 1988, p. 191-196.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go.
AU - Hinsch, K D
AU - Rosenthal, W
AU - Spicher, K
AU - Binder, Thomas
AU - Gausepohl, H
AU - Frank, R
AU - Schultz, G
AU - Joost, H G
PY - 1988
Y1 - 1988
N2 - Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go.
AB - Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go.
M3 - SCORING: Zeitschriftenaufsatz
VL - 238
SP - 191
EP - 196
JO - FEBS LETT
JF - FEBS LETT
SN - 0014-5793
IS - 1
M1 - 1
ER -