Addition of GM-CSF to a peptide/KLH vaccine results in increased frequencies of CXCR3-expressing KLH-specific T cells
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Addition of GM-CSF to a peptide/KLH vaccine results in increased frequencies of CXCR3-expressing KLH-specific T cells. / Na, Il-Kang; Keilholz, Ulrich; Letsch, Anne; Bauer, Sandra; Asemissen, Anne Marie; Nagorsen, Dirk; Thiel, Eckhard; Scheibenbogen, Carmen.
In: CANCER IMMUNOL IMMUN, Vol. 56, No. 3, 03.2007, p. 391-6.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Addition of GM-CSF to a peptide/KLH vaccine results in increased frequencies of CXCR3-expressing KLH-specific T cells
AU - Na, Il-Kang
AU - Keilholz, Ulrich
AU - Letsch, Anne
AU - Bauer, Sandra
AU - Asemissen, Anne Marie
AU - Nagorsen, Dirk
AU - Thiel, Eckhard
AU - Scheibenbogen, Carmen
PY - 2007/3
Y1 - 2007/3
N2 - T-cell trafficking is determined by expression patterns of chemokine receptors. The chemokine receptor CXCR3 is expressed on a subpopulation of type 1 T cells and plays an important role for migration of T cells into inflamed and tumor tissues. Here, we studied the chemokine receptor expression on specific T cells generated against the neoantigen keyhole limpet hemocyanin (KLH) in patients who had been immunized in the context of a tumor peptide vaccination trial with or without the adjuvant granulocyte-macrophage colony-stimulating factor (GM-CSF). In patients immunized in the presence of GM-CSF the fraction of CXCR3(+) KLH-specific T cells was significantly higher than in patients immunized in the absence of GM-CSF (median 45 vs. 20%, P = 0.001). In contrast, the chemokine receptor CCR4, associated with migration to the skin was found in both cohorts on less than 10% of KLH-specific T cells. These results show that CXCR3 expression on vaccine-induced T cells can be modulated by modifying the local vaccine milieu.
AB - T-cell trafficking is determined by expression patterns of chemokine receptors. The chemokine receptor CXCR3 is expressed on a subpopulation of type 1 T cells and plays an important role for migration of T cells into inflamed and tumor tissues. Here, we studied the chemokine receptor expression on specific T cells generated against the neoantigen keyhole limpet hemocyanin (KLH) in patients who had been immunized in the context of a tumor peptide vaccination trial with or without the adjuvant granulocyte-macrophage colony-stimulating factor (GM-CSF). In patients immunized in the presence of GM-CSF the fraction of CXCR3(+) KLH-specific T cells was significantly higher than in patients immunized in the absence of GM-CSF (median 45 vs. 20%, P = 0.001). In contrast, the chemokine receptor CCR4, associated with migration to the skin was found in both cohorts on less than 10% of KLH-specific T cells. These results show that CXCR3 expression on vaccine-induced T cells can be modulated by modifying the local vaccine milieu.
KW - Adjuvants, Immunologic/administration & dosage
KW - Cancer Vaccines/immunology
KW - Cell Line, Tumor
KW - Cohort Studies
KW - Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage
KW - Hemocyanins/administration & dosage
KW - Humans
KW - Injections, Intradermal
KW - Injections, Subcutaneous
KW - Melanoma/immunology
KW - Neoplasm Staging
KW - Peptides/administration & dosage
KW - Receptors, CXCR3
KW - Receptors, Chemokine/biosynthesis
KW - T-Lymphocytes/drug effects
U2 - 10.1007/s00262-006-0198-7
DO - 10.1007/s00262-006-0198-7
M3 - SCORING: Journal article
C2 - 16850346
VL - 56
SP - 391
EP - 396
JO - CANCER IMMUNOL IMMUN
JF - CANCER IMMUNOL IMMUN
SN - 0340-7004
IS - 3
ER -
