Research ExplorerPublicationsAcute Versus Chronic Administration of Calcineurin-Inhibitor...

Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function

Standard

Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function. / Kirchhof, Julia; Wilde, Benjamin; Schmidt, Justine; Mülling, Nils; Petrakova, Liubov; Brinkhoff, Alexandra; Schedlowski, Manfred; Witzke, Oliver.

In: ENDOCR METAB IMMUNE, Vol. 21, No. 6, 2021, p. 1083-1089.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kirchhof, J, Wilde, B, Schmidt, J, Mülling, N, Petrakova, L, Brinkhoff, A, Schedlowski, M & Witzke, O 2021, 'Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function', ENDOCR METAB IMMUNE, vol. 21, no. 6, pp. 1083-1089. https://doi.org/10.2174/1871530320999200831161710

APA

Kirchhof, J., Wilde, B., Schmidt, J., Mülling, N., Petrakova, L., Brinkhoff, A., Schedlowski, M., & Witzke, O. (2021). Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function. ENDOCR METAB IMMUNE, 21(6), 1083-1089. https://doi.org/10.2174/1871530320999200831161710

Vancouver

Kirchhof J, Wilde B, Schmidt J, Mülling N, Petrakova L, Brinkhoff A et al. Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function. ENDOCR METAB IMMUNE. 2021;21(6):1083-1089. https://doi.org/10.2174/1871530320999200831161710

Bibtex

@article{0926109364ba4a928b965a99210266ed,
title = "Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function",
abstract = "BACKGROUND: Calcineurin-inhibitors (CNI) are used in renal transplant patients (RTX) to prevent rejection. CNI mainly suppress T-cell mediated immunity but very little is known about the impact of long-term treatment with CNI on T-cell function.OBJECTIVE: We investigated the immunological effects of long-term CNI intake in RTX patients in comparison to short-term CNI administration in healthy controls (HC).METHODS: Blood was drawn from 30 RTX patients with long-term CNI treatment. In addition, blood was sampled from HC with short-term CNI treatment (four dosages) before the first and 2 hours after the last CsA intake. T-cells were analyzed for cytokine production, proliferation, and CD25 expression.RESULTS: Short-term CNI reduced T-cell derived IL-2 and IFNγ as well as T-cell proliferation in HC. IFNγ was not suppressed in patients with long-term CNI treatment. IL-2 production, CD25 expression, and T-cell proliferation were enhanced in long-term CNI patients.CONCLUSION: Suppression of IFNγ/IL-2 and T-cell proliferation is weaker during long-term CNI treatment in patients compared to short-term treatment in healthy subjects. Enhanced CD25 expression may lower the threshold for T-cell activation during long-term CNI treatment.",
keywords = "Adult, Aged, Calcineurin Inhibitors/administration & dosage, Cell Proliferation/drug effects, Drug Administration Schedule, Female, Flow Cytometry/methods, Graft Rejection/immunology, Humans, Immunosuppressive Agents/administration & dosage, Inflammation Mediators/antagonists & inhibitors, Kidney Transplantation/trends, Male, Middle Aged, T-Lymphocytes/drug effects",
author = "Julia Kirchhof and Benjamin Wilde and Justine Schmidt and Nils M{\"u}lling and Liubov Petrakova and Alexandra Brinkhoff and Manfred Schedlowski and Oliver Witzke",
note = "Copyright{\textcopyright} Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.",
year = "2021",
doi = "10.2174/1871530320999200831161710",
language = "English",
volume = "21",
pages = "1083--1089",
journal = "ENDOCR METAB IMMUNE",
issn = "1871-5303",
publisher = "Bentham Science Publishers B.V.",
number = "6",

}

RIS

TY - JOUR

T1 - Acute Versus Chronic Administration of Calcineurin-Inhibitors Differentially Affect T-Cell Function

AU - Kirchhof, Julia

AU - Wilde, Benjamin

AU - Schmidt, Justine

AU - Mülling, Nils

AU - Petrakova, Liubov

AU - Brinkhoff, Alexandra

AU - Schedlowski, Manfred

AU - Witzke, Oliver

N1 - Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

PY - 2021

Y1 - 2021

N2 - BACKGROUND: Calcineurin-inhibitors (CNI) are used in renal transplant patients (RTX) to prevent rejection. CNI mainly suppress T-cell mediated immunity but very little is known about the impact of long-term treatment with CNI on T-cell function.OBJECTIVE: We investigated the immunological effects of long-term CNI intake in RTX patients in comparison to short-term CNI administration in healthy controls (HC).METHODS: Blood was drawn from 30 RTX patients with long-term CNI treatment. In addition, blood was sampled from HC with short-term CNI treatment (four dosages) before the first and 2 hours after the last CsA intake. T-cells were analyzed for cytokine production, proliferation, and CD25 expression.RESULTS: Short-term CNI reduced T-cell derived IL-2 and IFNγ as well as T-cell proliferation in HC. IFNγ was not suppressed in patients with long-term CNI treatment. IL-2 production, CD25 expression, and T-cell proliferation were enhanced in long-term CNI patients.CONCLUSION: Suppression of IFNγ/IL-2 and T-cell proliferation is weaker during long-term CNI treatment in patients compared to short-term treatment in healthy subjects. Enhanced CD25 expression may lower the threshold for T-cell activation during long-term CNI treatment.

AB - BACKGROUND: Calcineurin-inhibitors (CNI) are used in renal transplant patients (RTX) to prevent rejection. CNI mainly suppress T-cell mediated immunity but very little is known about the impact of long-term treatment with CNI on T-cell function.OBJECTIVE: We investigated the immunological effects of long-term CNI intake in RTX patients in comparison to short-term CNI administration in healthy controls (HC).METHODS: Blood was drawn from 30 RTX patients with long-term CNI treatment. In addition, blood was sampled from HC with short-term CNI treatment (four dosages) before the first and 2 hours after the last CsA intake. T-cells were analyzed for cytokine production, proliferation, and CD25 expression.RESULTS: Short-term CNI reduced T-cell derived IL-2 and IFNγ as well as T-cell proliferation in HC. IFNγ was not suppressed in patients with long-term CNI treatment. IL-2 production, CD25 expression, and T-cell proliferation were enhanced in long-term CNI patients.CONCLUSION: Suppression of IFNγ/IL-2 and T-cell proliferation is weaker during long-term CNI treatment in patients compared to short-term treatment in healthy subjects. Enhanced CD25 expression may lower the threshold for T-cell activation during long-term CNI treatment.

KW - Adult

KW - Aged

KW - Calcineurin Inhibitors/administration & dosage

KW - Cell Proliferation/drug effects

KW - Drug Administration Schedule

KW - Female

KW - Flow Cytometry/methods

KW - Graft Rejection/immunology

KW - Humans

KW - Immunosuppressive Agents/administration & dosage

KW - Inflammation Mediators/antagonists & inhibitors

KW - Kidney Transplantation/trends

KW - Male

KW - Middle Aged

KW - T-Lymphocytes/drug effects

U2 - 10.2174/1871530320999200831161710

DO - 10.2174/1871530320999200831161710

M3 - SCORING: Journal article

C2 - 32867664

VL - 21

SP - 1083

EP - 1089

JO - ENDOCR METAB IMMUNE

JF - ENDOCR METAB IMMUNE

SN - 1871-5303

IS - 6

ER -