Abundance of follicular helper T cells in Peyer's patches is modulated by CD155

TY - JOUR

T1 - Abundance of follicular helper T cells in Peyer's patches is modulated by CD155

AU - Seth, Sebastian

AU - Ravens, Inga

AU - Kremmer, Elisabeth

AU - Maier, Michael K

AU - Hadis, Usriansyah

AU - Hardtke, Svenja

AU - Förster, Reinhold

AU - Bernhardt, Günter

PY - 2009/11

Y1 - 2009/11

N2 - The secondary humoral immune response is characterized by plasma B cells secreting isotype-switched and affinity-matured antibodies. The efficient generation of plasma B cells in the GC depends on the presence of follicular helper T (T(FH)) cells, a cell type thought to arise from naive CD4-positive T cells by a hitherto unresolved differentiation pathway. Mice deficient for CD155, an adhesion receptor of the immunoglobulin superfamily, are impaired to mount a secondary humoral immune response upon oral administration of antigen, while the primary IgM response is unaffected. Here, we show that mice lacking CD155 harbor significantly reduced numbers of T(FH) cells in their Peyer's patches. This was paralleled by a decreased frequency of T(FH) cells in the GC. Moreover, the CD155 ligand CD226, which is involved in T-cell activation, is down-regulated during T(FH) cell differentiation, resulting in a complete absence of CD226 on those T(FH) cells residing in the GC. Concurrently, the expression of TIGIT/WUCAM, a newly discovered CD155 ligand, is induced in T(FH) cells. Thus, these cells replace an activating by a putative inhibitory CD155-binding partner during their differentiation.

AB - The secondary humoral immune response is characterized by plasma B cells secreting isotype-switched and affinity-matured antibodies. The efficient generation of plasma B cells in the GC depends on the presence of follicular helper T (T(FH)) cells, a cell type thought to arise from naive CD4-positive T cells by a hitherto unresolved differentiation pathway. Mice deficient for CD155, an adhesion receptor of the immunoglobulin superfamily, are impaired to mount a secondary humoral immune response upon oral administration of antigen, while the primary IgM response is unaffected. Here, we show that mice lacking CD155 harbor significantly reduced numbers of T(FH) cells in their Peyer's patches. This was paralleled by a decreased frequency of T(FH) cells in the GC. Moreover, the CD155 ligand CD226, which is involved in T-cell activation, is down-regulated during T(FH) cell differentiation, resulting in a complete absence of CD226 on those T(FH) cells residing in the GC. Concurrently, the expression of TIGIT/WUCAM, a newly discovered CD155 ligand, is induced in T(FH) cells. Thus, these cells replace an activating by a putative inhibitory CD155-binding partner during their differentiation.

KW - Animals

KW - Antigens, Differentiation, T-Lymphocyte/immunology

KW - Cell Differentiation/immunology

KW - Cell Separation

KW - Female

KW - Flow Cytometry

KW - Germinal Center/cytology

KW - Immunomodulation/immunology

KW - Lymphocyte Activation/immunology

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Transgenic

KW - Peyer's Patches/cytology

KW - Receptors, Immunologic/immunology

KW - Receptors, Virus/immunology

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocytes, Helper-Inducer/cytology

U2 - 10.1002/eji.200939470

DO - 10.1002/eji.200939470

M3 - SCORING: Journal article

C2 - 19688744

VL - 39

SP - 3160

EP - 3170

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 11

ER -