Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin
Standard
Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin. / Ben Nasr, A; Herwald, H; Sjöbring, U; Renné, T; Müller-Esterl, W; Björck, L.
In: BIOCHEM J, Vol. 326 ( Pt 3), 15.09.1997, p. 657-60.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin
AU - Ben Nasr, A
AU - Herwald, H
AU - Sjöbring, U
AU - Renné, T
AU - Müller-Esterl, W
AU - Björck, L
PY - 1997/9/15
Y1 - 1997/9/15
N2 - H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.
AB - H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.
KW - Bradykinin
KW - Humans
KW - Kininogens
KW - Protein Binding
KW - Streptococcus pyogenes
M3 - SCORING: Journal article
C2 - 9307013
VL - 326 ( Pt 3)
SP - 657
EP - 660
JO - BIOCHEM J
JF - BIOCHEM J
SN - 0264-6021
ER -