Abscisic acid stimulates the release of insulin and of GLP-1 in the rat perfused pancreas and intestine
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Abscisic acid stimulates the release of insulin and of GLP-1 in the rat perfused pancreas and intestine. / Booz, Valeria; Christiansen, Charlotte Bayer; Kuhre, Rune Ehrenreich; Saltiel, Monika Yosifova; Sociali, Giovanna; Schaltenberg, Nicola; Fischer, Alexander W; Heeren, Jörg; Zocchi, Elena; Holst, Jens J; Bruzzone, Santina.
In: DIABETES-METAB RES, Vol. 35, No. 2, 02.2019, p. e3102.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Abscisic acid stimulates the release of insulin and of GLP-1 in the rat perfused pancreas and intestine
AU - Booz, Valeria
AU - Christiansen, Charlotte Bayer
AU - Kuhre, Rune Ehrenreich
AU - Saltiel, Monika Yosifova
AU - Sociali, Giovanna
AU - Schaltenberg, Nicola
AU - Fischer, Alexander W
AU - Heeren, Jörg
AU - Zocchi, Elena
AU - Holst, Jens J
AU - Bruzzone, Santina
N1 - © 2018 John Wiley & Sons, Ltd.
PY - 2019/2
Y1 - 2019/2
N2 - AIMS: Previous results indicate that nanomolar concentrations of abscisic acid (ABA) stimulate insulin release from β-pancreatic cells in vitro and that oral ABA at 50 mg/kg increases plasma GLP-1 in the fasted rat. The aim of this study was to test the effect of ABA on the perfused rat pancreas and intestine, to verify the insulin- and incretin-releasing actions of ABA in controlled physiological models.MATERIALS AND METHODS: Rat pancreas and small intestine were perfused with solutions containing ABA at high-micromolar concentrations, or control secretagogues. Insulin and GLP-1 concentrations in the venous effluent were analysed by radioimmunoassay, and ABA levels were determined by ELISA.RESULTS: High micromolar concentrations of ABA induced GLP-1 secretion from the proximal half of the small intestine and insulin secretion from pancreas. GLP-1 stimulated ABA secretion from pancreas in a biphasic manner. Notably, a positive correlation was found between the ABA area under the curve (AUC) and the insulin AUC upon GLP-1 administration.CONCLUSION: Our results indicate the existence of a cross talk between GLP-1 and ABA, whereby ABA stimulates GLP-1 secretion, and vice versa. Release of ABA could be considered as a new promising molecule in the strategy of type 2 diabetes treatment and as a new endogenous hormone in the regulation of glycaemia.
AB - AIMS: Previous results indicate that nanomolar concentrations of abscisic acid (ABA) stimulate insulin release from β-pancreatic cells in vitro and that oral ABA at 50 mg/kg increases plasma GLP-1 in the fasted rat. The aim of this study was to test the effect of ABA on the perfused rat pancreas and intestine, to verify the insulin- and incretin-releasing actions of ABA in controlled physiological models.MATERIALS AND METHODS: Rat pancreas and small intestine were perfused with solutions containing ABA at high-micromolar concentrations, or control secretagogues. Insulin and GLP-1 concentrations in the venous effluent were analysed by radioimmunoassay, and ABA levels were determined by ELISA.RESULTS: High micromolar concentrations of ABA induced GLP-1 secretion from the proximal half of the small intestine and insulin secretion from pancreas. GLP-1 stimulated ABA secretion from pancreas in a biphasic manner. Notably, a positive correlation was found between the ABA area under the curve (AUC) and the insulin AUC upon GLP-1 administration.CONCLUSION: Our results indicate the existence of a cross talk between GLP-1 and ABA, whereby ABA stimulates GLP-1 secretion, and vice versa. Release of ABA could be considered as a new promising molecule in the strategy of type 2 diabetes treatment and as a new endogenous hormone in the regulation of glycaemia.
KW - Journal Article
U2 - 10.1002/dmrr.3102
DO - 10.1002/dmrr.3102
M3 - SCORING: Journal article
C2 - 30468287
VL - 35
SP - e3102
JO - DIABETES-METAB RES
JF - DIABETES-METAB RES
SN - 1520-7552
IS - 2
ER -
