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Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss

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Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss. / Al-Rubaye, Saja; Ghaderian, Sayyed Mohammad Hossein; Salehpour, Saghar; Salmani, Tayyebali; Vojdani, Samaneh; Yaseen, Rusul; Akbarzadeh, Reza.

In: GYNECOL ENDOCRINOL, Vol. 37, No. 7, 07.2021, p. 660-664.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Al-Rubaye, S, Ghaderian, SMH, Salehpour, S, Salmani, T, Vojdani, S, Yaseen, R & Akbarzadeh, R 2021, 'Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss', GYNECOL ENDOCRINOL, vol. 37, no. 7, pp. 660-664. https://doi.org/10.1080/09513590.2021.1897098

APA

Al-Rubaye, S., Ghaderian, S. M. H., Salehpour, S., Salmani, T., Vojdani, S., Yaseen, R., & Akbarzadeh, R. (2021). Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss. GYNECOL ENDOCRINOL, 37(7), 660-664. https://doi.org/10.1080/09513590.2021.1897098

Vancouver

Al-Rubaye S, Ghaderian SMH, Salehpour S, Salmani T, Vojdani S, Yaseen R et al. Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss. GYNECOL ENDOCRINOL. 2021 Jul;37(7):660-664. https://doi.org/10.1080/09513590.2021.1897098

Bibtex

@article{a92448a095684b1ca3b875dd5f893d94,
title = "Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss",
abstract = "AIMS: Recurrent pregnancy loss (RPL), with unknown causes, is one of the most common challenges facing pregnancy. Apoptotic signaling pathways are involved in the normal and abnormal pregnancy process. Despite the evidence pointing toward the aberrant expression of apoptotic and apoptotic-related genes in pregnancy complications, the involvement of these genes in RPL remains to be elucidated. This study aimed to investigate the expression levels of BAX, MEG3, and miR-214-3p (as a microRNA), and their associations in an Iranian population.MATERIALS AND METHODS: Following the extraction of RNA from blood samples of RPL patients and controls, quantitative expression levels of BAX, MEG3, and miR-214-3p genes were analyzed by real-time RT-PCR.RESULTS: The findings showed that the expression levels of BAX and miRNA-214-3p were significantly higher in the blood samples of RPL patients than in control samples. In contrast, the expression of MEG3 was significantly down-regulated in women RPL. Furthermore, altered expressions of MEG3 and miRNA-214-3p are associated with their target gene BAX, where the BAX expression is positively and negatively correlated with the expressions of has-miR-214-3P and MEG3, respectively. ROC curve evaluation demonstrated the highest specificity and diagnostic value for miR-214-3p expression in distinguishing RPL samples from the healthy controls.CONCLUSIONS: These data indicated that the aberrant expression of BAX, MEG3, miRNA-214-3p genes in RPL patients could provide new insights into the biological characteristics and related pathways of differentially expressed genes, which could help as potential diagnostic biomarkers and a better understanding of the molecular mechanisms of RPL.",
keywords = "Abortion, Habitual/genetics, Adult, Female, Gene Expression, Humans, Iran, MicroRNAs/genetics, Pregnancy, RNA, Long Noncoding/genetics, RNA, Messenger/metabolism, bcl-2-Associated X Protein/genetics",
author = "Saja Al-Rubaye and Ghaderian, {Sayyed Mohammad Hossein} and Saghar Salehpour and Tayyebali Salmani and Samaneh Vojdani and Rusul Yaseen and Reza Akbarzadeh",
year = "2021",
month = jul,
doi = "10.1080/09513590.2021.1897098",
language = "English",
volume = "37",
pages = "660--664",
journal = "GYNECOL ENDOCRINOL",
issn = "0951-3590",
publisher = "informa healthcare",
number = "7",

}

RIS

TY - JOUR

T1 - Aberrant expression of BAX, MEG3, and miR-214-3P genes in recurrent pregnancy loss

AU - Al-Rubaye, Saja

AU - Ghaderian, Sayyed Mohammad Hossein

AU - Salehpour, Saghar

AU - Salmani, Tayyebali

AU - Vojdani, Samaneh

AU - Yaseen, Rusul

AU - Akbarzadeh, Reza

PY - 2021/7

Y1 - 2021/7

N2 - AIMS: Recurrent pregnancy loss (RPL), with unknown causes, is one of the most common challenges facing pregnancy. Apoptotic signaling pathways are involved in the normal and abnormal pregnancy process. Despite the evidence pointing toward the aberrant expression of apoptotic and apoptotic-related genes in pregnancy complications, the involvement of these genes in RPL remains to be elucidated. This study aimed to investigate the expression levels of BAX, MEG3, and miR-214-3p (as a microRNA), and their associations in an Iranian population.MATERIALS AND METHODS: Following the extraction of RNA from blood samples of RPL patients and controls, quantitative expression levels of BAX, MEG3, and miR-214-3p genes were analyzed by real-time RT-PCR.RESULTS: The findings showed that the expression levels of BAX and miRNA-214-3p were significantly higher in the blood samples of RPL patients than in control samples. In contrast, the expression of MEG3 was significantly down-regulated in women RPL. Furthermore, altered expressions of MEG3 and miRNA-214-3p are associated with their target gene BAX, where the BAX expression is positively and negatively correlated with the expressions of has-miR-214-3P and MEG3, respectively. ROC curve evaluation demonstrated the highest specificity and diagnostic value for miR-214-3p expression in distinguishing RPL samples from the healthy controls.CONCLUSIONS: These data indicated that the aberrant expression of BAX, MEG3, miRNA-214-3p genes in RPL patients could provide new insights into the biological characteristics and related pathways of differentially expressed genes, which could help as potential diagnostic biomarkers and a better understanding of the molecular mechanisms of RPL.

AB - AIMS: Recurrent pregnancy loss (RPL), with unknown causes, is one of the most common challenges facing pregnancy. Apoptotic signaling pathways are involved in the normal and abnormal pregnancy process. Despite the evidence pointing toward the aberrant expression of apoptotic and apoptotic-related genes in pregnancy complications, the involvement of these genes in RPL remains to be elucidated. This study aimed to investigate the expression levels of BAX, MEG3, and miR-214-3p (as a microRNA), and their associations in an Iranian population.MATERIALS AND METHODS: Following the extraction of RNA from blood samples of RPL patients and controls, quantitative expression levels of BAX, MEG3, and miR-214-3p genes were analyzed by real-time RT-PCR.RESULTS: The findings showed that the expression levels of BAX and miRNA-214-3p were significantly higher in the blood samples of RPL patients than in control samples. In contrast, the expression of MEG3 was significantly down-regulated in women RPL. Furthermore, altered expressions of MEG3 and miRNA-214-3p are associated with their target gene BAX, where the BAX expression is positively and negatively correlated with the expressions of has-miR-214-3P and MEG3, respectively. ROC curve evaluation demonstrated the highest specificity and diagnostic value for miR-214-3p expression in distinguishing RPL samples from the healthy controls.CONCLUSIONS: These data indicated that the aberrant expression of BAX, MEG3, miRNA-214-3p genes in RPL patients could provide new insights into the biological characteristics and related pathways of differentially expressed genes, which could help as potential diagnostic biomarkers and a better understanding of the molecular mechanisms of RPL.

KW - Abortion, Habitual/genetics

KW - Adult

KW - Female

KW - Gene Expression

KW - Humans

KW - Iran

KW - MicroRNAs/genetics

KW - Pregnancy

KW - RNA, Long Noncoding/genetics

KW - RNA, Messenger/metabolism

KW - bcl-2-Associated X Protein/genetics

U2 - 10.1080/09513590.2021.1897098

DO - 10.1080/09513590.2021.1897098

M3 - SCORING: Journal article

C2 - 33719810

VL - 37

SP - 660

EP - 664

JO - GYNECOL ENDOCRINOL

JF - GYNECOL ENDOCRINOL

SN - 0951-3590

IS - 7

ER -