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A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation

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A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation. / Kaufman, Charles K; Mosimann, Christian; Fan, Zi Peng; Yang, Song; Thomas, Andrew J; Ablain, Julien; Tan, Justin L; Fogley, Rachel D; van Rooijen, Ellen; Hagedorn, Elliott J; Ciarlo, Christie; White, Richard M; Matos, Dominick A; Puller, Ann-Christin; Santoriello, Cristina; Liao, Eric C; Young, Richard A; Zon, Leonard I.

In: SCIENCE, Vol. 351, No. 6272, 29.01.2016, p. aad2197.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kaufman, CK, Mosimann, C, Fan, ZP, Yang, S, Thomas, AJ, Ablain, J, Tan, JL, Fogley, RD, van Rooijen, E, Hagedorn, EJ, Ciarlo, C, White, RM, Matos, DA, Puller, A-C, Santoriello, C, Liao, EC, Young, RA & Zon, LI 2016, 'A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation', SCIENCE, vol. 351, no. 6272, pp. aad2197. https://doi.org/10.1126/science.aad2197

APA

Kaufman, C. K., Mosimann, C., Fan, Z. P., Yang, S., Thomas, A. J., Ablain, J., Tan, J. L., Fogley, R. D., van Rooijen, E., Hagedorn, E. J., Ciarlo, C., White, R. M., Matos, D. A., Puller, A-C., Santoriello, C., Liao, E. C., Young, R. A., & Zon, L. I. (2016). A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation. SCIENCE, 351(6272), aad2197. https://doi.org/10.1126/science.aad2197

Vancouver

Kaufman CK, Mosimann C, Fan ZP, Yang S, Thomas AJ, Ablain J et al. A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation. SCIENCE. 2016 Jan 29;351(6272):aad2197. https://doi.org/10.1126/science.aad2197

Bibtex

@article{44b5e89d95ac49d58e60d3313d95ef29,
title = "A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation",
abstract = "The {"}cancerized field{"} concept posits that cancer-prone cells in a given tissue share an oncogenic mutation, but only discreet clones within the field initiate tumors. Most benign nevi carry oncogenic BRAF(V600E) mutations but rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors (NCPs) and specifically reexpressed in melanoma. Live imaging of transgenic zebrafish crestin reporters shows that within a cancerized field (BRAF(V600E)-mutant; p53-deficient), a single melanocyte reactivates the NCP state, revealing a fate change at melanoma initiation in this model. NCP transcription factors, including sox10, regulate crestin expression. Forced sox10 overexpression in melanocytes accelerated melanoma formation, which is consistent with activation of NCP genes and super-enhancers leading to melanoma. Our work highlights NCP state reemergence as a key event in melanoma initiation.",
keywords = "Animals, Animals, Genetically Modified, Carcinogenesis, Embryonic Stem Cells, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Gene Expression Regulation, Neoplastic, Genes, Reporter, Green Fluorescent Proteins, Melanocytes, Melanoma, Melanoma, Experimental, Mutation, Nerve Tissue Proteins, Neural Crest, Proto-Oncogene Proteins B-raf, SOXE Transcription Factors, Skin Neoplasms, Tumor Suppressor Protein p53, Zebrafish, Zebrafish Proteins, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Kaufman, {Charles K} and Christian Mosimann and Fan, {Zi Peng} and Song Yang and Thomas, {Andrew J} and Julien Ablain and Tan, {Justin L} and Fogley, {Rachel D} and {van Rooijen}, Ellen and Hagedorn, {Elliott J} and Christie Ciarlo and White, {Richard M} and Matos, {Dominick A} and Ann-Christin Puller and Cristina Santoriello and Liao, {Eric C} and Young, {Richard A} and Zon, {Leonard I}",
note = "Copyright {\textcopyright} 2016, American Association for the Advancement of Science.",
year = "2016",
month = jan,
day = "29",
doi = "10.1126/science.aad2197",
language = "English",
volume = "351",
pages = "aad2197",
journal = "SCIENCE",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6272",

}

RIS

TY - JOUR

T1 - A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation

AU - Kaufman, Charles K

AU - Mosimann, Christian

AU - Fan, Zi Peng

AU - Yang, Song

AU - Thomas, Andrew J

AU - Ablain, Julien

AU - Tan, Justin L

AU - Fogley, Rachel D

AU - van Rooijen, Ellen

AU - Hagedorn, Elliott J

AU - Ciarlo, Christie

AU - White, Richard M

AU - Matos, Dominick A

AU - Puller, Ann-Christin

AU - Santoriello, Cristina

AU - Liao, Eric C

AU - Young, Richard A

AU - Zon, Leonard I

N1 - Copyright © 2016, American Association for the Advancement of Science.

PY - 2016/1/29

Y1 - 2016/1/29

N2 - The "cancerized field" concept posits that cancer-prone cells in a given tissue share an oncogenic mutation, but only discreet clones within the field initiate tumors. Most benign nevi carry oncogenic BRAF(V600E) mutations but rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors (NCPs) and specifically reexpressed in melanoma. Live imaging of transgenic zebrafish crestin reporters shows that within a cancerized field (BRAF(V600E)-mutant; p53-deficient), a single melanocyte reactivates the NCP state, revealing a fate change at melanoma initiation in this model. NCP transcription factors, including sox10, regulate crestin expression. Forced sox10 overexpression in melanocytes accelerated melanoma formation, which is consistent with activation of NCP genes and super-enhancers leading to melanoma. Our work highlights NCP state reemergence as a key event in melanoma initiation.

AB - The "cancerized field" concept posits that cancer-prone cells in a given tissue share an oncogenic mutation, but only discreet clones within the field initiate tumors. Most benign nevi carry oncogenic BRAF(V600E) mutations but rarely become melanoma. The zebrafish crestin gene is expressed embryonically in neural crest progenitors (NCPs) and specifically reexpressed in melanoma. Live imaging of transgenic zebrafish crestin reporters shows that within a cancerized field (BRAF(V600E)-mutant; p53-deficient), a single melanocyte reactivates the NCP state, revealing a fate change at melanoma initiation in this model. NCP transcription factors, including sox10, regulate crestin expression. Forced sox10 overexpression in melanocytes accelerated melanoma formation, which is consistent with activation of NCP genes and super-enhancers leading to melanoma. Our work highlights NCP state reemergence as a key event in melanoma initiation.

KW - Animals

KW - Animals, Genetically Modified

KW - Carcinogenesis

KW - Embryonic Stem Cells

KW - Enhancer Elements, Genetic

KW - Gene Expression Regulation, Developmental

KW - Gene Expression Regulation, Neoplastic

KW - Genes, Reporter

KW - Green Fluorescent Proteins

KW - Melanocytes

KW - Melanoma

KW - Melanoma, Experimental

KW - Mutation

KW - Nerve Tissue Proteins

KW - Neural Crest

KW - Proto-Oncogene Proteins B-raf

KW - SOXE Transcription Factors

KW - Skin Neoplasms

KW - Tumor Suppressor Protein p53

KW - Zebrafish

KW - Zebrafish Proteins

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1126/science.aad2197

DO - 10.1126/science.aad2197

M3 - SCORING: Journal article

C2 - 26823433

VL - 351

SP - aad2197

JO - SCIENCE

JF - SCIENCE

SN - 0036-8075

IS - 6272

ER -